IgM antibody

Sheep anti Bovine IgM

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgM

Product Code Applications Pack Size List Price Your Price Qty
AAI19
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E ID 1 mg loader
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Sheep anti bovine IgM recognizes bovine IgM and shows no cross-reactivity with other bovine immunoglobulin classes in immunoelectrophoresis. Sheep anti bovine IgM may cross-react with IgM from other species.

Target Species
Bovine
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to bovine IgM were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Purified bovine IgM.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/1000
Immunodiffusion
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

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References for IgM antibody

  1. Nebl, T. et al. (2002) Proteomic analysis of a detergent-resistant membrane skeleton from neutrophil plasma membranes.
    J Biol Chem. 277 (45): 43399-409.
  2. Assad, A. et al. (2012) Immunophenotyping and characterization of BNP colostra revealed pathogenic alloantibodies of IgG1 subclass with specifity to platelets, granulocytes and monocytes of all maturation stages.
    Vet Immunol Immunopathol. 147: 25-34.
  3. Hamsten, C. et al. (2009) Recombinant surface proteomics as a tool to analyze humoral immune responses in bovines infected by Mycoplasma mycoides subsp. mycoides small colony type.
    Mol Cell Proteomics. 8: 2544-54.
  4. Mansilla FC et al. (2015) Safety and immunogenicity of a soluble native Neospora caninum tachyzoite-extract vaccine formulated with a soy lecithin/β-glucan adjuvant in pregnant cattle.
    Vet Immunol Immunopathol. 165 (1-2): 75-80.
  5. Hossain, M.M. et al. (2016) Multiplex Detection of IgG and IgM to Rift Valley Fever Virus Nucleoprotein, Nonstructural Proteins, and Glycoprotein in Ovine and Bovine.
    Vector Borne Zoonotic Dis. Jul 5. [Epub ahead of print]
  6. Van Meulder, F. et al. (2015) Analysis of the protective immune response following intramuscular vaccination of calves against the intestinal parasite Cooperia oncophora.
    Int J Parasitol. 45 (9-10): 637-46.
  7. Jankowska, A. et al. (2016) Humoral and cellular immune response to Histophilus somni recombinant heat shock protein 60 kDa in farm animals
    Veterinární Medicína. 60 (No. 11): 603-12.
  8. Çokçalışkan, C. et al. (2019) Effect of simultaneous administration of foot-and-mouth disease (FMD) and anthrax vaccines on antibody response to FMD in sheep.
    Clin Exp Vaccine Res. 8 (2): 103-9.
  9. View The Latest Product References
  10. Springer, A. et al. (2022) Immunization Trials with Recombinant Major Sperm Protein of the Bovine Lungworm Dictyocaulus viviparus..
    Pathogens 2022, 11, 55.

ELISA

Western Blotting

RRID
AB_323077
View more products with IGM specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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