IgM antibody
Horseradish peroxidase conjugated Sheep anti Bovine IgM antibody (AAI19P) used as a detection antibody in an ELISA to evaluate IgM responses to D.viviparus. infection.
Image caption:
Anti-rMSP antibody development during vaccination trials. Mean optical density (OD) values are displayed over the course of the study for immunoglobulin (Ig) classes IgG, IgA and IgM for the different study groups. Error bars indicate standard deviations. V = vaccination, C = challenge infection.
From: Springer, A.; von Holtum, C.; von Samson-Himmelstjerna, G.; Strube, C.
Immunization Trials with Recombinant Major Sperm Protein of the Bovine Lungworm Dictyocaulus viviparus.
Pathogens 2022, 11, 55.
doi: 10.3390/pathogens11010055
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Sheep anti Bovine IgM
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgM
Antibody Quality and Performance Guarantee
| Sheep anti bovine IgM recognizes bovine IgM and shows no cross-reactivity with other bovine immunoglobulin classes in immunoelectrophoresis. Sheep anti bovine IgM may cross-react with IgM from other species. |
- Target Species
- Bovine
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to bovine IgM were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Purified bovine IgM.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/100 | 1/1000 |
| Immunodiffusion | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C E F WB | 1.5 ml |
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| List Price | Your Price | ||||||
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| Description | Rabbit anti Sheep IgG (H/L):Biotin | ||||||
References for IgM antibody
-
Nebl, T. et al. (2002) Proteomic analysis of a detergent-resistant membrane skeleton from neutrophil plasma membranes.
J Biol Chem. 277 (45): 43399-409. -
Assad, A. et al. (2012) Immunophenotyping and characterization of BNP colostra revealed pathogenic alloantibodies of IgG1 subclass with specifity to platelets, granulocytes and monocytes of all maturation stages.
Vet Immunol Immunopathol. 147: 25-34. -
Hamsten, C. et al. (2009) Recombinant surface proteomics as a tool to analyze humoral immune responses in bovines infected by Mycoplasma mycoides subsp. mycoides small colony type.
Mol Cell Proteomics. 8: 2544-54. -
Mansilla FC et al. (2015) Safety and immunogenicity of a soluble native Neospora caninum tachyzoite-extract vaccine formulated with a soy lecithin/β-glucan adjuvant in pregnant cattle.
Vet Immunol Immunopathol. 165 (1-2): 75-80. -
Hossain, M.M. et al. (2016) Multiplex Detection of IgG and IgM to Rift Valley Fever Virus Nucleoprotein, Nonstructural Proteins, and Glycoprotein in Ovine and Bovine.
Vector Borne Zoonotic Dis. 16 (8): 550-7. -
Van Meulder, F. et al. (2015) Analysis of the protective immune response following intramuscular vaccination of calves against the intestinal parasite Cooperia oncophora.
Int J Parasitol. 45 (9-10): 637-46. -
Jankowska, A. et al. (2016) Humoral and cellular immune response to Histophilus somni recombinant heat shock protein 60 kDa in farm animals
Veterinární Medicína. 60 (No. 11): 603-12. -
Di Giacomo, S et al. (2022) Assessment on Different Vaccine Formulation Parameters in the Protection against Heterologous Challenge with FMDV in Cattle
Viruses. 14 (8): 1781.
View The Latest Product References
-
Springer, A. et al. (2022) Immunization Trials with Recombinant Major Sperm Protein of the Bovine Lungworm Dictyocaulus viviparus..
Pathogens 2022, 11, 55.
- RRID
- AB_323077
View more products with IgM specificity
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