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IgG antibody

Rabbit anti Sheep IgG (H/L):Biotin

Product Type
Polyclonal Antibody
Polyclonal IgG

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Biotinylated Rabbit anti Sheep IgG polyclonal antibody recognizes intact ovine IgG, raised against the whole IgG molecule it may recognize epitpoes encompassing both heavy and light chains. The antibody has not been cross adsorbed and may react with immunoglobulins of other subtypes and from other species.

Biotinylated Rabbit anti Sheep IgG polyclonal antibody is a useful and robust reagent for the detection of bound sheep antibodies with labeled streptavidin as a tertiary reagent.

Biotinylated Rabbit anti Sheep IgG polyclonal antibody has been used successfully for evaluation of IgG responses in vaccinated and challenged sheep (Ramos et al. 2018).

Target Species
Product Form
Purified IgG conjugated to Biotin - lyophilized
Reconstitute in 1.5 ml sterile distilled water.
Purified IgG prepared by immunoaffinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.05%Sodium Azide (NaN3)
1.5%Bovine Serum Albumin
Whole sheep IgG.
For research purposes only
12 months from date of despatch

Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/20,000 1/400,000
Flow Cytometry 1/200 1/1,000
Immunohistology - Frozen 1/500 1/5,000
Western Blotting 1/20,000 1/400,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

References for IgG antibody

  1. Ramos, A. et al. (2018) Melatonin enhances responsiveness to Dichelobacter nodosus. vaccine in sheep and increases peripheral blood CD4 T lymphocytes and IgG-expressing B lymphocytes.
    Vet Immunol Immunopathol. 206: 1-8.



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