IgG2 antibody

Sheep anti Bovine IgG2

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG2

Product Code Applications Pack Size List Price Your Price Qty
AAI22
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SDS Safety Datasheet SDS
E ID 1 mg loader
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Sheep anti Bovine IgG2 polyclonal antibody recognizes bovine IgG2.

No cross - reactivity with other bovine immunoglobulin classes is seen in immunoelectrophoresis. This product may cross-react with IgG2 from other species.

Target Species
Bovine
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to bovine IgG2 were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Purified bovine IgG2.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/10000
Immunodiffusion
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

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References for IgG2 antibody

  1. Makepeace, B.L. et al. (2009) Immunisation with a multivalent, subunit vaccine reduced patent infection in a natural bovine model of Onchocerciasis during intense field exposure.
    PLoS Negl. Trop. Dis. 3: e544.
  2. Colwell, D.D. & Goater, C.P. (2010) Dicrocoelium dendriticum in cattle from Cypress Hills, Canada: humoral response and preliminary evaluation of an ELISA.
    Vet Parasitol. 174 (1-2): 162-5.
  3. Assad, A. et al. (2012) Immunophenotyping and characterization of BNP colostra revealed pathogenic alloantibodies of IgG1 subclass with specifity to platelets, granulocytes and monocytes of all maturation stages.
    Vet Immunol Immunopathol. 147: 25-34.
  4. Agnes,J.T. et al. (2011) Identification of Anaplasma marginale Outer Membrane Protein Antigens Conserved between A. marginale Sensu Stricto Strains and the Live A. marginale subsp. centrale Vaccine
    Infect Immun. 79: 1311-8.
  5. Lavoria, M.Á. et al. (2012) Avidity and subtyping of specific antibodies applied to the indirect assessment of heterologous protection against Foot-and-Mouth Disease Virus in cattle.
    Vaccine. 30: 6845-50.
  6. Vordermeier, H.M. et al. (2003) Improved immunogenicity of DNA vaccination with mycobacterial HSP65 against bovine tuberculosis by protein boosting.
    Vet Microbiol. 93: 349-59.
  7. Mansilla, F.C. et al. (2013) Dose-dependent immunogenicity of a soluble Neospora caninum tachyzoite-extract vaccine formulated with a soy lecithin/β-glucan adjuvant in cattle.
    Vet Parasitol. 197 (1-2): 13-21.
  8. Panadero, R. et al. (2013) Effect of reinfestations on systemic immune responses in cattle naturally infested by Hypoderma sp. (Diptera: Oestridae).
    Vet Parasitol. 193: 238-44.
  9. View The Latest Product References
  10. Ploegaert, T.C. et al. (2010) Genetic variation of natural antibodies in milk of Dutch Holstein-Friesian cows.
    J Dairy Sci. 93: 5467-73.
  11. Van Meulder, F. et al. (2013) Granule exocytosis of granulysin and granzyme B as a potential key mechanism in vaccine-induced immunity in cattle against the nematode Ostertagia ostertagi.
    Infect Immun. 81: 1798-809.
  12. Maree, F.F. et al. (2015) Intra-serotype SAT2 chimeric foot-and-mouth disease vaccine protects cattle against FMDV challenge.
    Vaccine. 33 (25): 2909-16.
  13. Rybarczyk, J. et al. (2017) Effects of lactoferrin treatment on Escherichia coli O157:H7 rectal colonization in cattle.
    Vet Microbiol. 202: 38-46.
  14. González-Hernández A et al. (2016) Host protective ASP-based vaccine against the parasitic nematode Ostertagia ostertagi triggers NK cell activation and mixed IgG1-IgG2 response.
    Sci Rep. 6: 29496.
  15. Pecora, A. et al. (2015) Development of an APC-targeted multivalent E2-based vaccine against Bovine Viral Diarrhea Virus types 1 and 2.
    Vaccine. 33 (39): 5163-71.
  16. Scott, K.A. et al. (2017) Evaluation of immune responses of stabilised SAT2 antigens of foot-and-mouth disease in cattle.
    Vaccine. 35 (40): 5426-33.
  17. Sheng, Z.A. et al. (2019) Th2-related cytokines are associated with Fasciola gigantica infection and evasion in the natural host, swamp buffalo.
    Vet Parasitol. 268: 73-80.
  18. Bucafusco, D .et al. (2019) Immune cells transferred by colostrum do not influence the immune responses to foot-and-mouth disease primary vaccination.
    J Dairy Sci. 102 (9): 8376-84.
  19. Jiménez-Pelayo, L. et al. (2019) Early Neospora caninum infection dynamics in cattle after inoculation at mid-gestation with high (Nc-Spain7)- or low (Nc-Spain1H)-virulence isolates.
    Vet Res. 50 (1): 72.
  20. Rainard, P. et al. (2017) Cellular and humoral immune response to recombinant Escherichia coli. OmpA in cows.
    PLoS One. 12 (10): e0187369.
  21. Springer, A.et al. (2022) Immunization Trials with Recombinant Major Sperm Protein of the Bovine Lungworm Dictyocaulus viviparus..
    Pathogens 2022, 11, 55.

Western Blotting

RRID
AB_323068
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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