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IgG2 antibody

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Anti Bovine IgG2 Antibody thumbnail image 1 Anti Bovine IgG2 Antibody thumbnail image 2 Anti Bovine IgG2 Antibody thumbnail image 3
Anti Bovine IgG2 Antibody gallery image 1
Published customer image:
HRP conjugated Sheep anti Bovine IgG2 antibody (AAI22P) used as a detection reagent in a sandwich ELISA for the evaluation of Onchocerca ochengi specific IgG2 responses in cattle following vaccination.
Image caption:
Total area-under-curve (OD405 nm) for IgG1 and IgG2 responses to eight Onchocerca ochengi recombinant antigens.
‘Vaccinated, patent’ (n = 7) or ‘vaccinated, non-patent’ (n = 5) refers to presence or absence of dermal microfilariae at 22 months post-exposure, respectively; all adjuvant-control animals had patent infections (n = 13). Area-under-curve was calculated from data obtained at 0, 4 and 21 months post-exposure. Box-and-whisker plots display the median line, 25th–75th IQR (box), highest and lowest values within 1.5× IQR (whiskers), outliers (○; >1.5–3.0×IQR) and extreme values (▹; >3.0×IQR).

From: Makepeace BL, Jensen SA, Laney SJ, Nfon CK, Njongmeta LM, Tanya VN, et al. (2009)
Immunisation with a Multivalent, Subunit Vaccine Reduces Patent Infection in a Natural Bovine Model of Onchocerciasis during Intense Field Exposure.
PLoS Negl Trop Dis 3(11): e544.
https://doi.org/10.1371/journal.pntd.0000544.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Bovine IgG2 Antibody gallery image 2
Published customer image:
HRP conjugated Sheep anti Bovine IgG2 antibody (AAI22P) used as a detection reagent in a sandwich ELISA for the evaluation of antigen specific IgG2 antibody responses in cattle following vaccination against nematode antigens.
Image caption:
Detection of nASP-specific IgG1 and IgG2 antibodies in blood and mucosa after vaccination of cattle with nASP+QuilA and pASP+QuilA.
In cattle study 1, (A) serum and (B) abomasal samples were collected from all animals and used for the detection of nASP specific IgG1 and IgG2 type antibodies through ELISA. The graphs show the individual and mean (± SEM) OD's for each individual animal within each group ± SEM. Statistically significant differences are indicated with *p < 0.05.

From:González-Hernández A, Van Coppernolle S, Borloo J, Van Meulder F, Paerewijck O, Peelaers I, Leclercq G, Claerebout E, Geldhof P.
Host protective ASP-based vaccine against the parasitic nematode Ostertagia ostertagi triggers NK cell activation and mixed IgG1-IgG2 response.
Sci Rep. 2016 Jul 11;6:29496.
doi:10.1038/srep29496.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Bovine IgG2 Antibody gallery image 3
Published customer image:
Horseradish peroxidase conjugated Sheep anti Bovine IgG2 antibody (AAI22P) used for the evaluation of IgG2 levels in bovine serum samples by ELISA.
Image caption:
N. caninum-specific humoural immune responses. Serum levels of IgG2 antibodies against N. caninum in G-Control, G-NcSpain7 and G-NcSpain1H. Immunoglobulin levels are expressed as a relative index percent (RIPC) according to RIPC = (OD405 sample − OD405 negative control)/(OD405 positive control − OD405 negative control) × 100. Each point represents the mean + SD at different sampling times. The red line indicates the ELISA cut-off point from which samples are considered positive. **** and ** indicate P <0.0001 and P <0.001 significant differences.

From: Jiménez-Pelayo, L.
Early Neospora caninum infection dynamics in cattle after inoculation at mid-gestation with high (Nc-Spain7)- or low (Nc-Spain1H)-virulence isolates.
Vet Res. 2019 Sep 24;50(1):72.
doi: 10.1186/s13567-019-0691-6.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Sheep anti Bovine IgG2:Alk. Phos.

Sheep anti Bovine IgG2:FITC

Sheep anti Bovine IgG2:HRP

Sheep anti Bovine IgG2

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AAI22AB
Datasheet
E
MSDS
0.5 mg loader

AAI22F
Datasheet
C F
MSDS
1 mg loader

AAI22P
Datasheet
E
MSDS
1 mg loader

AAI22
Datasheet
E ID
MSDS
1 mg loader

Sheep anti Bovine IgG2 polyclonal antibody recognizes bovine IgG2.

No cross - reactivity with other bovine immunoglobulin classes is seen in immunoelectrophoresis. This product may cross-react with IgG2 from other species.

Product Details

Target Species
Bovine
Product Form
Purified IgG conjugated to Alkaline Phosphatase - liquid
Product Form
Purified IgG fraction conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to bovine IgG2 were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
50mM HEPES, 0.1M NaCl, 1mM MgCl2, 0.1mM ZnCl2
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.05% Proclin™ 300
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Purified bovine IgG2.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC.
DO NOT FREEZE.

This product should be stored undiluted.
Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch.
Guarantee
12 months from date of despatch.
Guarantee
12 months from date of despatch.
Guarantee
18 months from date of despatch.

More Information

Acknowledgements
Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
Regulatory
For research purposes only

Applications of IgG2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000 1/10000
Flow Cytometry 1/20 1/100
Immunohistology - Frozen 1/20 1/100
ELISA 1/10,000 1/100,000
ELISA 1/100 1/10000
Immunodiffusion
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 C E F WB 1.5 ml loader
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA F IF 0.1 mg loader
Donkey anti Sheep/Goat IgG:FITC STAR88F C 1 mg loader
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
TMB Signal+ BUF054A E 100 ml
TMB Core BUF056A E 100 ml
TMB Core+ BUF062A E 100 ml

Application Based External Images

Western Blotting

Product Specific References

References for IgG2 antibody

  1. Makepeace, B.L. et al. (2009) Immunisation with a multivalent, subunit vaccine reduced patent infection in a natural bovine model of Onchocerciasis during intense field exposure.
    PLoS Negl. Trop. Dis. 3: e544.
  2. Colwell, D.D. & Goater, C.P. (2010) Dicrocoelium dendriticum in cattle from Cypress Hills, Canada: humoral response and preliminary evaluation of an ELISA.
    Vet Parasitol. 174 (1-2): 162-5.
  3. Assad, A. et al. (2012) Immunophenotyping and characterization of BNP colostra revealed pathogenic alloantibodies of IgG1 subclass with specifity to platelets, granulocytes and monocytes of all maturation stages.
    Vet Immunol Immunopathol. 147: 25-34.
  4. Agnes,J.T. et al. (2011) Identification of Anaplasma marginale Outer Membrane Protein Antigens Conserved between A. marginale Sensu Stricto Strains and the Live A. marginale subsp. centrale Vaccine
    Infect Immun. 79: 1311-8.
  5. Lavoria, M.Á. et al. (2012) Avidity and subtyping of specific antibodies applied to the indirect assessment of heterologous protection against Foot-and-Mouth Disease Virus in cattle.
    Vaccine. 30: 6845-50.
  6. Vordermeier, H.M. et al. (2003) Improved immunogenicity of DNA vaccination with mycobacterial HSP65 against bovine tuberculosis by protein boosting.
    Vet Microbiol. 93: 349-59.
  7. Mansilla, F.C. et al. (2013) Dose-dependent immunogenicity of a soluble Neospora caninum tachyzoite-extract vaccine formulated with a soy lecithin/β-glucan adjuvant in cattle.
    Vet Parasitol. 197 (1-2): 13-21.
  8. Panadero, R. et al. (2013) Effect of reinfestations on systemic immune responses in cattle naturally infested by Hypoderma sp. (Diptera: Oestridae).
    Vet Parasitol. 193: 238-44.
  9. Ploegaert, T.C. et al. (2010) Genetic variation of natural antibodies in milk of Dutch Holstein-Friesian cows.
    J Dairy Sci. 93: 5467-73.
  10. Van Meulder, F. et al. (2013) Granule exocytosis of granulysin and granzyme B as a potential key mechanism in vaccine-induced immunity in cattle against the nematode Ostertagia ostertagi.
    Infect Immun. 81: 1798-809.
  11. Maree, F.F. et al. (2015) Intra-serotype SAT2 chimeric foot-and-mouth disease vaccine protects cattle against FMDV challenge.
    Vaccine. 33 (25): 2909-16.
  12. Rybarczyk, J. et al. (2017) Effects of lactoferrin treatment on Escherichia coli O157:H7 rectal colonization in cattle.
    Vet Microbiol. 202: 38-46.
  13. González-Hernández A et al. (2016) Host protective ASP-based vaccine against the parasitic nematode Ostertagia ostertagi triggers NK cell activation and mixed IgG1-IgG2 response.
    Sci Rep. 6: 29496.
  14. Pecora, A. et al. (2015) Development of an APC-targeted multivalent E2-based vaccine against Bovine Viral Diarrhea Virus types 1 and 2.
    Vaccine. 33 (39): 5163-71.
  15. Scott, K.A. et al. (2017) Evaluation of immune responses of stabilised SAT2 antigens of foot-and-mouth disease in cattle.
    Vaccine. 35 (40): 5426-33.
  16. Sheng, Z.A. et al. (2019) Th2-related cytokines are associated with Fasciola gigantica infection and evasion in the natural host, swamp buffalo.
    Vet Parasitol. 268: 73-80.
  17. Bucafusco, D .et al. (2019) Immune cells transferred by colostrum do not influence the immune responses to foot-and-mouth disease primary vaccination.
    J Dairy Sci. 102 (9): 8376-84.
  18. Jiménez-Pelayo, L. et al. (2019) Early Neospora caninum infection dynamics in cattle after inoculation at mid-gestation with high (Nc-Spain7)- or low (Nc-Spain1H)-virulence isolates.
    Vet Res. 50 (1): 72.
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