IgG2 antibody
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Sheep anti Bovine IgG2 polyclonal antibody recognizes bovine IgG2.
No cross - reactivity with other bovine immunoglobulin classes is seen in immunoelectrophoresis. This product may cross-react with IgG2 from other species.
No cross - reactivity with other bovine immunoglobulin classes is seen in immunoelectrophoresis. This product may cross-react with IgG2 from other species.
Product Details
- Target Species
- Bovine
- Product Form
- Purified IgG conjugated to Alkaline Phosphatase - liquid
- Product Form
- Purified IgG fraction conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to bovine IgG2 were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- 50mM HEPES, 0.1M NaCl, 1mM MgCl2, 0.1mM ZnCl2
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
- 0.1% Proclin™ 300
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Purified bovine IgG2.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted.
Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of despatch.
- Shelf Life
- 12 months from date of despatch.
- Shelf Life
- 12 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
More Information
- Acknowledgements
- Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
- Regulatory
- For research purposes only
Applications of IgG2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/1000 | 1/10000 | |
| Flow Cytometry | 1/20 | 1/100 | |
| Immunohistology - Frozen | 1/20 | 1/100 | |
| ELISA | 1/10,000 | 1/100,000 | |
| ELISA | 1/100 | 1/10000 | |
| Immunodiffusion |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | 1.5 ml | C E F WB | ||
| Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | 0.1 mg | F IF | ||
| Donkey anti Sheep/Goat IgG:DyLight®549 | STAR88D549GA | 0.1 mg | F IF | ||
| Donkey anti Sheep/Goat IgG:DyLight®649 | STAR88D649GA | 0.1 mg | F IF | ||
| Donkey anti Sheep/Goat IgG:FITC | STAR88F | 1 mg | C | ||
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | 100 ml | C E P WB | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | 500 ml | C E P WB | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | 1000 ml | C E P WB | ||
| TMB Signal+ | BUF054A | 100 ml | E | ||
| TMB Core | BUF056A | 100 ml | E | ||
| TMB Core+ | BUF062A | 100 ml | E |
Product Specific References
References for IgG2 antibody
-
Makepeace, B.L. et al. (2009) Immunisation with a multivalent, subunit vaccine reduced patent infection in a natural bovine model of Onchocerciasis during intense field exposure.
PLoS Negl. Trop. Dis. 3: e544. -
Colwell, D.D. & Goater, C.P. (2010) Dicrocoelium dendriticum in cattle from Cypress Hills, Canada: humoral response and preliminary evaluation of an ELISA.
Vet Parasitol. 174 (1-2): 162-5. -
Assad, A. et al. (2012) Immunophenotyping and characterization of BNP colostra revealed pathogenic alloantibodies of IgG1 subclass with specifity to platelets, granulocytes and monocytes of all maturation stages.
Vet Immunol Immunopathol. 147: 25-34. -
Agnes,J.T. et al. (2011) Identification of Anaplasma marginale Outer Membrane Protein Antigens Conserved between A. marginale Sensu Stricto Strains and the Live A. marginale subsp. centrale Vaccine
Infect Immun. 79: 1311-8. -
Lavoria, M.Á. et al. (2012) Avidity and subtyping of specific antibodies applied to the indirect assessment of heterologous protection against Foot-and-Mouth Disease Virus in cattle.
Vaccine. 30: 6845-50. -
Vordermeier, H.M. et al. (2003) Improved immunogenicity of DNA vaccination with mycobacterial HSP65 against bovine tuberculosis by protein boosting.
Vet Microbiol. 93: 349-59. -
Mansilla, F.C. et al. (2013) Dose-dependent immunogenicity of a soluble Neospora caninum tachyzoite-extract vaccine formulated with a soy lecithin/β-glucan adjuvant in cattle.
Vet Parasitol. 197 (1-2): 13-21. -
Panadero, R. et al. (2013) Effect of reinfestations on systemic immune responses in cattle naturally infested by Hypoderma sp. (Diptera: Oestridae).
Vet Parasitol. 193: 238-44. -
Ploegaert, T.C. et al. (2010) Genetic variation of natural antibodies in milk of Dutch Holstein-Friesian cows.
J Dairy Sci. 93: 5467-73. -
Van Meulder, F. et al. (2013) Granule exocytosis of granulysin and granzyme B as a potential key mechanism in vaccine-induced immunity in cattle against the nematode Ostertagia ostertagi.
Infect Immun. 81: 1798-809. -
Maree, F.F. et al. (2015) Intra-serotype SAT2 chimeric foot-and-mouth disease vaccine protects cattle against FMDV challenge.
Vaccine. 33 (25): 2909-16. -
Rybarczyk, J. et al. (2015) Effects of lactoferrin treatment on Escherichia coli O157:H7 rectal colonization in cattle.
Vet Microbiol. pii: S0378-1135(15)30119-X. [Epub ahead of print] -
González-Hernández A et al. (2016) Host protective ASP-based vaccine against the parasitic nematode Ostertagia ostertagi triggers NK cell activation and mixed IgG1-IgG2 response.
Sci Rep. 6: 29496. -
Pecora, A. et al. (2015) Development of an APC-targeted multivalent E2-based vaccine against Bovine Viral Diarrhea Virus types 1 and 2.
Vaccine. 33 (39): 5163-71. -
Scott, K.A. et al. (2017) Evaluation of immune responses of stabilised SAT2 antigens of foot-and-mouth disease in cattle.
Vaccine. Apr 18 [Epub ahead of print].
