IgG1 antibody

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Sheep anti Bovine IgG1:Alk. Phos.

Sheep anti Bovine IgG1:Biotin

Sheep anti Bovine IgG1:FITC

Sheep anti Bovine IgG1:HRP

Sheep anti Bovine IgG1

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AAI21AB E 0.5 mg
AAI21B E WB 1 mg
AAI21F C F 1 mg
AAI21P E 1 mg
AAI21 E ID 1 mg
Sheep anti Bovine IgG1 polyclonal antibody recognizes bovine IgG1.

No cross-reactivity with other bovine immunoglobulin classes is seen in immunoelectrophoresis.This product may cross-react with IgG1 from other species.

Product Details

Target Species
Bovine
Product Form
Purified IgG conjugated to Alkaline Phosphatase - liquid
Product Form
Purified IgG fraction conjugated to Biotin - liquid
Product Form
Purified IgG fraction conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to bovine IgG1 were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
50mM HEPES, 0.1M NaCl, 1mM MgCl2, 0.1mM ZnCl2
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.05% Proclin™ 300
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Purified bovine IgG1.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC.
DO NOT FREEZE.

This product should be stored undiluted.
Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of despatch.
Shelf Life
12 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

Acknowledgements
Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
Regulatory
For research purposes only

Applications of IgG1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000 1/10000
ELISA 1:10000 1:100000
Western Blotting 1:10000 1:100000
Flow Cytometry 1/20 1/100
Immunohistology - Frozen 1/20 1/100
ELISA 1/10,000 1/100,000
ELISA
Immunodiffusion
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 1.5 ml C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®549 STAR88D549GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®649 STAR88D649GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:FITC STAR88F 1 mg C
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Application Based External Images

ELISA

Product Specific References

References for IgG1 antibody

  1. Makepeace, B.L. et al. (2009) Immunisation with a multivalent, subunit vaccine reduced patent infection in a natural bovine model of Onchocerciasis during intense field exposure.
    PLoS Negl. Trop. Dis. 3: e544.
  2. Colwell, D.D. et al. (2010) Dicrocoelium dendriticum in cattle from Cypress Hills, Canada: Humoral response and preliminary evaluation of an ELISA.
    Vet Parasitol. 174: 162-5.
  3. Assad, A. et al. (2012) Immunophenotyping and characterization of BNP colostra revealed pathogenic alloantibodies of IgG1 subclass with specifity to platelets, granulocytes and monocytes of all maturation stages.
    Vet Immunol Immunopathol. 147: 25-34.
  4. Ploegaert, T.C. et al. (2010) Genetic variation of natural antibodies in milk of Dutch Holstein-Friesian cows.
    J Dairy Sci. 93: 5467-73.
  5. Lavoria, M.Á. et al. (2012) Avidity and subtyping of specific antibodies applied to the indirect assessment of heterologous protection against Foot-and-Mouth Disease Virus in cattle.
    Vaccine. 30: 6845-50.
  6. Mansilla, F.C. et al. (2013) Dose-dependent immunogenicity of a soluble Neospora caninum tachyzoite-extract vaccine formulated with a soy lecithin/β-glucan adjuvant in cattle.
    Vet Parasitol. pii: S0304-4017(13)00252-5.
  7. Panadero, R. et al. (2013) Effect of reinfestations on systemic immune responses in cattle naturally infested by Hypoderma sp. (Diptera: Oestridae).
    Vet Parasitol. 193: 238-44.
  8. Van Meulder, F. et al. (2013) Granule exocytosis of granulysin and granzyme B as a potential key mechanism in vaccine-induced immunity in cattle against the nematode Ostertagia ostertagi.
    Infect Immun. 81: 1798-809.
  9. Vordermeier, H.M. et al. (2003) Improved immunogenicity of DNA vaccination with mycobacterial HSP65 against bovine tuberculosis by protein boosting.
    Vet Microbiol. 93: 349-59.
  10. Hansen, E.R. et al. (1989) Cutaneous T-cell lymphoma lesional epidermal cells activate autologous CD4+ T lymphocytes: involvement of both CD1+OKM5+ and CD1+OKM5- antigen-presenting cells.
    J Invest Dermatol. 94: 485-91.
  11. Fiedor, C. et al. (2009) Evaluation of a milk ELISA for the serodiagnosis of Dictyocaulus viviparus in dairy cows.
    Vet Parasitol. 166: 255-61.
  12. Almería, S. et al. (2009) Specific anti-Neospora caninum IgG1 and IgG2 antibody responses during gestation in naturally infected cattle and their relationship with gamma interferon production.
    Vet Immunol Immunopathol. 130: 35-42.
  13. TrottaMyri, a.n. et al. (2015) Simultaneous immunization of cattle with foot-and-mouth disease (FMD) and live anthrax vaccines do not interfere with FMD booster responses
    Trials in Vaccinology. 4: 38-42.
  14. Prado, M.E. et al. (2011) Vaccination of dairy cows with recombinant Streptococcus uberis adhesion molecule induces antibodies that reduce adherence to and internalization of S. uberis into bovine mammary epithelial cells.
    Vet Immunol Immunopathol. 141: 201-8.
  15. von Holtum, C. et al. (2008) Development and evaluation of a recombinant antigen-based ELISA for serodiagnosis of cattle lungworm.
    Vet Parasitol. 151: 218-26.
  16. Van Neerven, R.J. et al. (2010) Milk derived antigen-specific antibodies, methods of preparation and uses thereof.
    US Patent application no: US20100129377 A1
  17. Grit, G.H. et al. (2014) Evaluation of cellular and humoral systemic immune response against Giardia duodenalis infection in cattle.
    Vet Parasitol. 202: 145-55.
  18. van Diemen, P.M. et al. (2007) Subunit vaccines based on intimin and Efa-1 polypeptides induce humoral immunity in cattle but do not protect against intestinal colonisation by enterohaemorrhagic Escherichia coli O157:H7 or O26:H-.
    Vet Immunol Immunopathol. 116: 47-58.
  19. Riffault, S. et al. (2010) A new subunit vaccine based on nucleoprotein nanoparticles confers partial clinical and virological protection in calves against bovine respiratory syncytial virus.
    Vaccine. 28: 3722-34.
  20. Vázquez, L. et al. (2012) Antigen-specific antibody isotypes, lymphocyte subsets and cytokine profiles in cattle naturally infested by Hypoderma sp. (Diptera: Oestridae).
    Vet Parasitol. 184: 230-7.
  21. Patarroyo, J.H. et al. (2009) Immune response of bovines stimulated by synthetic vaccine SBm7462 against Rhipicephalus (Boophilus) microplus.
    Vet Parasitol. 166: 333-9.
  22. Maree, F.F. et al. (2015) Intra-serotype SAT2 chimeric foot-and-mouth disease vaccine protects cattle against FMDV challenge.
    Vaccine. 33 (25): 2909-16.
  23. Rybarczyk, J. et al. (2015) Effects of lactoferrin treatment on Escherichia coli O157:H7 rectal colonization in cattle.
    Vet Microbiol. pii: S0378-1135(15)30119-X. [Epub ahead of print]
  24. Bautista-Garfias, C.R. et al. (2015) Co-immunization of cattle with a vaccine against babesiosis and Lactobacillus casei increases specific IgG1 levels to Babesia bovis and B. bigemina.
    Parasitol Int. 64 (5): 319-23.
  25. González-Hernández A et al. (2016) Host protective ASP-based vaccine against the parasitic nematode Ostertagia ostertagi triggers NK cell activation and mixed IgG1-IgG2 response.
    Sci Rep. 6: 29496.
  26. Scott, K.A. et al. (2017) Evaluation of immune responses of stabilised SAT2 antigens of foot-and-mouth disease in cattle.
    Vaccine. Apr 18 [Epub ahead of print].