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IgM antibody

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Anti Chicken IgM Antibody thumbnail image 1 Anti Chicken IgM Antibody thumbnail image 2 Anti Chicken IgM Antibody thumbnail image 3
Anti Chicken IgM Antibody gallery image 1
Published customer image:
Horseradish peroxidase conjugated Goat anti Chicken IgM antibody (AAI27P) used for the evaluation of flagellin specific IgM response in exposed chickens by ELISA.
Image caption:
Antigen-specific serum antibody responses.
NHC flagellin-specific antibody levels in the groups immunized with (A) 40 μg of NHC protein and (B) 20 μg of NHC protein, both compared to levels in the solvent injected control group. Antigen-specific IgY, IgM or IgA antibody levels were determined in 20-fold diluted sera collected from 12 day-old individual animals. Results are displayed as a box-and-whisker plot (S/R, sample to reference ratio). An asterisk indicates statistically significant differences (α<0.05) between compared groups.

From: Radomska KA, Vaezirad MM, Verstappen KM, Wösten MMSM, Wagenaar JA, van Putten JPM (2016)
Chicken Immune Response after In Ovo Immunization with Chimeric TLR5 Activating Flagellin of Campylobacter jejuni.
PLoS ONE 11(10): e0164837.This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Chicken IgM Antibody gallery image 2
Published customer image:
Horseradish peroxidase conjugated Goat anti Chicken IgM antibody (AAI27P) used for the evaluation of flagellin specific IgM response in exposed chickens by ELISA.
Image caption:
Kinetics of NHC-specific antibody levels following in ovo vaccination.
ELISA results demonstrating the NHC flagellin-specific reactivity of serially diluted sera collected from (A, C, E) 12 day-old and (B, D, F) 18 day-old chickens injected with 40 μg of NHC flagellin or solvent (control). Serum IgY (A, B), IgM (C, D) and IgA (E, F) antibody responses are indicated. Graphs represent the analysis of individual animals, displayed as a box-and-whisker plot. The amount of antigen-specific antibodies was expressed in a relation to the reference sera (S/R, sample to reference ratio). An asterisk indicates statistically significant differences (α<0.05) between groups.

From: Radomska KA, Vaezirad MM, Verstappen KM, Wösten MMSM, Wagenaar JA, van Putten JPM (2016)
Chicken Immune Response after In Ovo Immunization with Chimeric TLR5 Activating Flagellin of Campylobacter jejuni.
PLoS ONE 11(10): e0164837.This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Chicken IgM Antibody gallery image 3
Published customer image:
FITC conjugated Goat anti Chicken IgM antibody (AAI27F) used to evaluate of the number of IgM expressing B cells on Pigeon mononuclear cells by flow cytometry.
Image caption:
Percentage of IgM+ B cells at 4, 7 and 14 dpi in healthy pigeons administered herbal extracts (left) and in pigeons administered herbal extracts and inoculated with PPMV-1 (right)

From: Dziewulska D, Stenzel T, Śmiałek M, Tykałowski B, Koncicki A.
The impact of Aloe vera and licorice extracts on selected mechanisms of humoral and cell-mediated immunity in pigeons experimentally infected with PPMV-1.
BMC Vet Res. 2018 May 2;14(1):148.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Goat anti Chicken IgM:FITC

Goat anti Chicken IgM:HRP

Goat anti Chicken IgM

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AAI27F
Datasheet
C F
MSDS
1 mg loader

AAI27P
Datasheet
C E IC WB
MSDS
1 mg loader

AAI27
Datasheet
C E IC ID WB
MSDS
1 mg loader

Goat anti Chicken IgM antibody recognizes chicken immunoglobulin M and shows no cross reactivity with other chicken immunoglobulin classes in immunoelectrophoresis.

Goat anti chicken IgM antibody may cross react with IgM from other species.

Product Details

Target Species
Chicken
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to chicken IgM were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
0.2%Bovine Serum Albumin
Preservative Stabilisers
0.05% Proclin™ 300
0.2% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified chicken IgM.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch.
Guarantee
12 months from date of despatch.
Guarantee
12 months from date of despatch.

More Information

UniProt
P01875
GO Terms
GO:0003823 antigen binding
GO:0030890 positive regulation of B cell proliferation
Acknowledgements
Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
Regulatory
For research purposes only

Applications of IgM antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen 1/200 1/2,000
ELISA 1/10,000 1/100,000
Immunocytochemistry 1/200 1/2000
Immunohistology - Frozen 1/200 1/2000
Western Blotting 1/2000 1/20,000
ELISA 1/100 1/1000
Immunocytochemistry 1/200 1/2000
Immunodiffusion
Immunohistology - Frozen 1/200 1/2000
Western Blotting 1/1000 1/30,000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
TMB Signal+ BUF054A E 100 ml
TMB Core BUF056A E 100 ml
TMB Core+ BUF062A E 100 ml

Application Based External Images

ELISA

Product Specific References

References for IgM antibody

  1. Withanage, G.S. et al. (2001) Cytokine and chemokine responses associated with clearance of a primary Salmonella enterica serovar Typhimurium infection in the chicken and in protective immunity to rechallenge.
    Infect Immun.73: 5173-82.
  2. Beal, R.K. et al. (2005) A strong antigen-specific T-cell response is associated with age and genetically dependent resistance to avian enteric salmonellosis.
    Infect Immun. 73 (11): 7509-16.
  3. Rautenschlein, S. et al. (2011) Local and systemic immune responses following infection of broiler-type chickens with avian Metapneumovirus subtypes A and B.
    Vet Immunol Immunopathol. 140(1-2):10-22.
  4. Toscano, M.J. et al. (2010) Sub-clinical infection with Salmonella in chickens differentially affects behaviour and welfare in three inbred strains.
    Br Poult Sci. 51: 703-13.
  5. Radomska, K.A. et al. (2016) Chicken Immune Response after In Ovo Immunization with Chimeric TLR5 Activating Flagellin of Campylobacter jejuni.
    PLoS One. 11 (10): e0164837.
  6. Dziewulska, D. et al. (2018) The impact of Aloe vera and licorice extracts on selected mechanisms of humoral and cell-mediated immunity in pigeons experimentally infected with PPMV-1.
    BMC Vet Res. 14 (1): 148.
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