IgM antibody
Horseradish peroxidase conjugated Goat anti Chicken IgM antibody (AAI27P) used for the evaluation of flagellin specific IgM response in exposed chickens by ELISA.
Image caption:
Antigen-specific serum antibody responses.
NHC flagellin-specific antibody levels in the groups immunized with (A) 40 μg of NHC protein and (B) 20 μg of NHC protein, both compared to levels in the solvent injected control group. Antigen-specific IgY, IgM or IgA antibody levels were determined in 20-fold diluted sera collected from 12 day-old individual animals. Results are displayed as a box-and-whisker plot (S/R, sample to reference ratio). An asterisk indicates statistically significant differences (α<0.05) between compared groups.
From: Radomska KA, Vaezirad MM, Verstappen KM, Wösten MMSM, Wagenaar JA, van Putten JPM (2016)
Chicken Immune Response after In Ovo Immunization with Chimeric TLR5 Activating Flagellin of Campylobacter jejuni.
PLoS ONE 11(10): e0164837.This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Horseradish peroxidase conjugated Goat anti Chicken IgM antibody (AAI27P) used for the evaluation of flagellin specific IgM response in exposed chickens by ELISA.
Image caption:
Kinetics of NHC-specific antibody levels following in ovo vaccination.
ELISA results demonstrating the NHC flagellin-specific reactivity of serially diluted sera collected from (A, C, E) 12 day-old and (B, D, F) 18 day-old chickens injected with 40 μg of NHC flagellin or solvent (control). Serum IgY (A, B), IgM (C, D) and IgA (E, F) antibody responses are indicated. Graphs represent the analysis of individual animals, displayed as a box-and-whisker plot. The amount of antigen-specific antibodies was expressed in a relation to the reference sera (S/R, sample to reference ratio). An asterisk indicates statistically significant differences (α<0.05) between groups.
From: Radomska KA, Vaezirad MM, Verstappen KM, Wösten MMSM, Wagenaar JA, van Putten JPM (2016)
Chicken Immune Response after In Ovo Immunization with Chimeric TLR5 Activating Flagellin of Campylobacter jejuni.
PLoS ONE 11(10): e0164837.This is from an open access article distributed under the terms of the Creative Commons Attribution License.
FITC conjugated Goat anti Chicken IgM antibody (AAI27F) used to evaluate of the number of IgM expressing B cells on Pigeon mononuclear cells by flow cytometry.
Image caption:
Percentage of IgM+ B cells at 4, 7 and 14 dpi in healthy pigeons administered herbal extracts (left) and in pigeons administered herbal extracts and inoculated with PPMV-1 (right)
From: Dziewulska D, Stenzel T, Śmiałek M, Tykałowski B, Koncicki A.
The impact of Aloe vera and licorice extracts on selected mechanisms of humoral and cell-mediated immunity in pigeons experimentally infected with PPMV-1.
BMC Vet Res. 2018 May 2;14(1):148.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Goat anti Chicken IgM antibody recognizes chicken immunoglobulin M and shows no cross reactivity with other chicken immunoglobulin classes in immunoelectrophoresis.
Goat anti chicken IgM antibody may cross react with IgM from other species.
Goat anti chicken IgM antibody may cross react with IgM from other species.
Product Details
- Target Species
- Chicken
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to chicken IgM were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 0.2% Bovine Serum Albumin - Preservative Stabilisers
- 0.05% Proclin™ 300
0.2% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Purified chicken IgM.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of despatch.
- Shelf Life
- 12 months from date of despatch.
- Shelf Life
- 12 months from date of despatch.
More Information
- UniProt
- P01875 Related reagents
- GO Terms
- GO:0003823 antigen binding
- GO:0030890 positive regulation of B cell proliferation
- Acknowledgements
- Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
- Regulatory
- For research purposes only
Applications of IgM antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | |||
| Immunohistology - Frozen | 1/200 | 1/2,000 | |
| ELISA | 1/10,000 | 1/100,000 | |
| Immunocytochemistry | 1/200 | 1/2000 | |
| Immunohistology - Frozen | 1/200 | 1/2000 | |
| Western Blotting | 1/2000 | 1/20,000 | |
| ELISA | 1/100 | 1/1000 | |
| Immunocytochemistry | 1/200 | 1/2000 | |
| Immunodiffusion | |||
| Immunohistology - Frozen | 1/200 | 1/2000 | |
| Western Blotting | 1/1000 | 1/30,000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | 1 mg | F | ||
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | 1 mg | C E WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | 100 ml | C E P WB | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | 500 ml | C E P WB | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | 1000 ml | C E P WB | ||
| TMB Signal+ | BUF054A | 100 ml | E | ||
| TMB Core | BUF056A | 100 ml | E | ||
| TMB Core+ | BUF062A | 100 ml | E |
Product Specific References
References for IgM antibody
-
Withanage, G.S. et al. (2001) Cytokine and chemokine responses associated with clearance of a primary Salmonella enterica serovar Typhimurium infection in the chicken and in protective immunity to rechallenge.
Infect Immun.73: 5173-82. -
Beal,R.K. et al (2005) A Strong Antigen-Specific T-Cell Response Is Associated with Age and Genetically Dependent Resistance to Avian Enteric Salmonellosis.
Infect. Immun. 2005 73: 7509-7516 -
Rautenschlein, S. et al. (2011) Local and systemic immune responses following infection of broiler-type chickens with avian Metapneumovirus subtypes A and B.
Vet Immunol Immunopathol. 140(1-2):10-22. -
Toscano, M.J. et al. (2010) Sub-clinical infection with Salmonella in chickens differentially affects behaviour and welfare in three inbred strains.
Br Poult Sci. 51: 703-13. -
Radomska, K.A. et al. (2016) Chicken Immune Response after In Ovo Immunization with Chimeric TLR5 Activating Flagellin of Campylobacter jejuni.
PLoS One. 11 (10): e0164837. -
Dziewulska, D. et al. (2018) The impact of Aloe vera and licorice extracts on selected mechanisms of humoral and cell-mediated immunity in pigeons experimentally infected with PPMV-1.
BMC Vet Res. 14 (1): 148.
