IgM antibody

Goat anti Chicken IgM

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgM

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AAI27
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SDS Safety Datasheet SDS
C E IC ID WB 1 mg loader
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Goat anti Chicken IgM antibody recognizes chicken immunoglobulin M and shows no cross reactivity with other chicken immunoglobulin classes in immunoelectrophoresis.

Goat anti chicken IgM antibody may cross react with IgM from other species.

Target Species
Chicken
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to chicken IgM were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified chicken IgM.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/1000
Immunocytochemistry 1/200 1/2000
Immunodiffusion
Immunohistology - Frozen 1/200 1/2000
Western Blotting 1/1000 1/30,000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

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References for IgM antibody

  1. Withanage, G.S. et al. (2001) Cytokine and chemokine responses associated with clearance of a primary Salmonella enterica serovar Typhimurium infection in the chicken and in protective immunity to rechallenge.
    Infect Immun.73: 5173-82.
  2. Beal, R.K. et al. (2005) A strong antigen-specific T-cell response is associated with age and genetically dependent resistance to avian enteric salmonellosis.
    Infect Immun. 73 (11): 7509-16.
  3. Rautenschlein, S. et al. (2011) Local and systemic immune responses following infection of broiler-type chickens with avian Metapneumovirus subtypes A and B.
    Vet Immunol Immunopathol. 140(1-2):10-22.
  4. Toscano, M.J. et al. (2010) Sub-clinical infection with Salmonella in chickens differentially affects behaviour and welfare in three inbred strains.
    Br Poult Sci. 51: 703-13.
  5. Radomska, K.A. et al. (2016) Chicken Immune Response after In Ovo Immunization with Chimeric TLR5 Activating Flagellin of Campylobacter jejuni.
    PLoS One. 11 (10): e0164837.
  6. Dziewulska, D. et al. (2018) The impact of Aloe vera and licorice extracts on selected mechanisms of humoral and cell-mediated immunity in pigeons experimentally infected with PPMV-1.
    BMC Vet Res. 14 (1): 148.
  7. Konieczka, P. et al. (2022) Increased arginine, lysine, and methionine levels can improve the performance, gut integrity and immune status of turkeys but the effect is interactive and depends on challenge conditions.
    Vet Res. 53 (1): 59.

ELISA

RRID
AB_2076334
UniProt
P01875
GO Terms
GO:0003823 antigen binding
GO:0030890 positive regulation of B cell proliferation
View more products with IGM specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Chicken Products