IgM antibody

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Goat anti Chicken IgM:FITC

Goat anti Chicken IgM:HRP

Goat anti Chicken IgM

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AAI27F C F 1 mg
AAI27P C E IC WB 1 mg
AAI27 C E IC ID WB 1 mg
Goat anti Chicken IgM antibody recognizes chicken immunoglobulin M and shows no cross reactivity with other chicken immunoglobulin classes in immunoelectrophoresis.

Goat anti chicken IgM antibody may cross react with IgM from other species.

Product Details

Target Species
Chicken
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to chicken IgM were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
0.2%Bovine Serum Albumin
Preservative Stabilisers
0.05% Proclin™ 300
0.2% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified chicken IgM.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.
Shelf Life
12 months from date of despatch.
Shelf Life
12 months from date of despatch.

More Information

UniProt
P01875 Related reagents
GO Terms
GO:0003823 antigen binding
GO:0030890 positive regulation of B cell proliferation
Acknowledgements
Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
Regulatory
For research purposes only

Applications of IgM antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen 1/200 1/2,000
ELISA 1/10,000 1/100,000
Immunocytochemistry 1/200 1/2000
Immunohistology - Frozen 1/200 1/2000
Western Blotting 1/2000 1/20,000
ELISA 1/100 1/1000
Immunocytochemistry 1/200 1/2000
Immunodiffusion
Immunohistology - Frozen 1/200 1/2000
Western Blotting 1/1000 1/30,000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F 1 mg F
Rabbit anti Goat IgG (Fc):HRP STAR122P 1 mg C E WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Application Based External Images

ELISA

Product Specific References

References for IgM antibody

  1. Withanage, G.S. et al. (2001) Cytokine and chemokine responses associated with clearance of a primary Salmonella enterica serovar Typhimurium infection in the chicken and in protective immunity to rechallenge.
    Infect Immun.73: 5173-82.
  2. Beal, R.K. et al. (2005) A strong antigen-specific T-cell response is associated with age and genetically dependent resistance to avian enteric salmonellosis.
    Infect Immun. 73 (11): 7509-16.
  3. Rautenschlein, S. et al. (2011) Local and systemic immune responses following infection of broiler-type chickens with avian Metapneumovirus subtypes A and B.
    Vet Immunol Immunopathol. 140(1-2):10-22.
  4. Toscano, M.J. et al. (2010) Sub-clinical infection with Salmonella in chickens differentially affects behaviour and welfare in three inbred strains.
    Br Poult Sci. 51: 703-13.
  5. Radomska, K.A. et al. (2016) Chicken Immune Response after In Ovo Immunization with Chimeric TLR5 Activating Flagellin of Campylobacter jejuni.
    PLoS One. 11 (10): e0164837.
  6. Dziewulska, D. et al. (2018) The impact of Aloe vera and licorice extracts on selected mechanisms of humoral and cell-mediated immunity in pigeons experimentally infected with PPMV-1.
    BMC Vet Res. 14 (1): 148.