IgG antibody

Goat anti Chicken IgG (Fc)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG
Region
(Fc)

Product Code Applications Pack Size List Price Your Price Qty
AAI29
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SDS Safety Datasheet SDS
E ID 1 mg loader
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Goat anti Chicken IgG (Fc) antibody recognizes the heavy chains of chicken IgG, specifically, epitopes within the Fc region and shows no cross-reactivity with other chicken immunoglobulin classes as assessed by immunoelectrophoresis. This Goat anti Chicken IgG polyclonal antibody does not react with the light chains of chicken IgG.

Goat anti chicken IgG (Fc) has been used successfully for the evaluation of circulating levels of IgG in chickens using ELISA in a number of experimental and field situations.

Target Species
Chicken
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to chicken IgG were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified chicken IgG (Fc) fragment.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/10000
Immunodiffusion
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

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References for IgG antibody

  1. Norup, L.R. et al. (2009) Influence of chicken serum mannose-binding lectin levels on the immune response towards Escherichia coli.
    Poult Sci. 88:543-53.
  2. Duckworth, J.A. et al. (2008) Development of a contraceptive vaccine for the marsupial brushtail possum (Trichosurus vulpecula): lack of effects in mice and chickens immunised with recombinant possum ZP3 protein and a possum ZP3 antifertility epitope
    Wildlife Research 35, 563–72.
  3. Pleidrup, J. et al. (2014) Ascaridia galli infection influences the development of both humoral and cell-mediated immunity after Newcastle Disease vaccination in chickens.
    Vaccine. 32 (3): 383-92.
  4. Peralta, B. et al. (2009) Evidence of widespread infection of avian hepatitis E virus (avian HEV) in chickens from Spain.
    Vet Microbiol. 137: 31-6
  5. Cho Y et al. (2015) Proteomic analysis of outer membrane proteins in Salmonella enterica Enteritidis.
    J Microbiol Biotechnol. 25 (2): 288-95.
  6. Ferdushy, T. et al. (2014) Acquisition of resistance after continuous infection with Ascaridia galli. in chickens.
    Parasitology. : 1-8.
  7. Radomska KA et al. (2016) Chicken Immune Response after In Ovo Immunization with Chimeric TLR5 Activating Flagellin of Campylobacter jejuni.
    PLoS One. 11 (10): e0164837.
  8. Vaezirad, M.M. et al. (2018) Chicken immune response following in ovo delivery of bacterial flagellin.
    Vaccine. Mar 09 [Epub ahead of print].
  9. View The Latest Product References
  10. Al-karagoly, H. et al. (2019) Turkey humoral and cell-mediated immune responses to a Newcastle viscerotropic vaccine and its association with major histocompatibility complex.
    Bulg J Vet Med. 22 (1): 26-40.
  11. Ranchod, H. et al. (2018) The antigenicity and cholesteroid nature of mycolic acids determined by recombinant chicken antibodies.
    PLoS One. 13 (8): e0200298.
  12. Naghizadeh, M. et al. (2019) Rapid whole blood assay using flow cytometry for measuring phagocytic activity of chicken leukocytes.
    Vet Immunol Immunopathol. 207: 53-61.
  13. Tang, B. et al. (2020) GtxA is a virulence factor that promotes a Th2-like response during Gallibacterium anatis. infection in laying hens.
    Vet Res. 51 (1): 40.

Synonyms
IgY
RRID
AB_323047
View more products with IGG specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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