IFN Gamma antibody

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Rabbit anti Chicken Interferon Gamma:Preservative Free

Product Type
Polyclonal Antibody
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
0.5 mg loader

Rabbit anti Chicken interferon gamma antibody recognizes chicken interferon gamma (chIFN-gamma). There are at least three distinct chicken interferon proteins, designated as chIFN-alpha, chIFN-beta and chIFN-gamma.

chIFN-gamma was first identified, when supernatant from chicken spleen cells stimulated with concanavalin A (ConA ), displayed macrophage-activating factor (MAF) activity - the principle function of mammalian type II IFN-gamma (Vonbulow et al. 1984). Cloning of IFN-gamma cDNA from activated chicken T cells indicates that recombinant chicken IFN-gamma activates macrophages, strongly upregulating the expression of MHC class II and iNOS genes (Weining et al. 1996).

Rabbit anti Chicken interferon gamma antibody can be used to neutralize chIFN-gamma activity and to discriminate the antiviral activity of chIFN-gamma from that of chIFN-alpha and chIFN-beta (Weining et al. 1996).

Product Details

Target Species
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to chicken Interferon Gamma were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography on protein A.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present.
Recombinant chicken interferon gamma
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
12 months from date of despatch

More Information

Entrez Gene
GO Terms
GO:0009615 response to virus
GO:0005125 cytokine activity
GO:0005133 interferon-gamma receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
For research purposes only

Applications of IFN Gamma antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays 10ug/ml 50ug/ml
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP945Z detects a band of approximately 18 kDa.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

References for IFN Gamma antibody

  1. Wei, Y. et al. (2016) Generation and protective efficacy of a cold-adapted attenuated avian H9N2 influenza vaccine.
    Sci Rep. 6: 30382.
  2. Andersen, S.H. et al. (2017) Quantification and phenotypic characterisation of peripheral IFN-γ producing leucocytes in chickens vaccinated against Newcastle disease.
    Vet Immunol Immunopathol. 193-194: 18-28.
  3. Wang, M. et al. (2018) Cross- immunity of a H9N2 live attenuated influenza vaccine against H5N2 highly pathogenic avian influenza virus in chickens.
    Vet Microbiol. 220: 57-66.

Further Reading

  1. Staeheli, P. et al. (2001) Cytokines of birds: conserved functions--a largely different look.
    J Interferon Cytokine Res. 21 (12): 993-1010.
  2. Weining, K.C. et al. (1996) Biological properties of recombinant chicken interferon-gamma.
    Eur J Immunol. 26 (10): 2440-7.
  3. Schultz, U. et al. (1995) Recombinant chicken interferon: a potent antiviral agent that lacks intrinsic macrophage activating factor activity.
    Eur J Immunol. 25 (3): 847-51.

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