Rabbit anti Chicken interferon gamma antibody
recognizes chicken interferon gamma (chIFN-gamma). There are at least three distinct chicken interferon proteins, designated as chIFN-alpha, chIFN-beta and chIFN-gamma.
chIFN-gamma was first identified, when supernatant from chicken spleen cells stimulated with concanavalin A (ConA ), displayed macrophage-activating factor (MAF) activity - the principle function of mammalian type II IFN-gamma (Vonbulow et al. 1984
). Cloning of IFN-gamma cDNA from activated chicken T cells indicates that recombinant chicken IFN-gamma activates macrophages, strongly upregulating the expression of MHC class II and iNOS genes (Weining et al. 1996
Rabbit anti Chicken interferon gamma antibody can be used to neutralize chIFN-gamma activity and to discriminate the antiviral activity of chIFN-gamma from that of chIFN-alpha and chIFN-beta (Weining et al. 1996
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to chicken Interferon Gamma were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography on protein A.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present.
- Recombinant chicken interferon gamma
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
response to virus
interferon-gamma receptor binding
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IFN Gamma antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP945Z detects a band of approximately 18 kDa.
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Secondary Antibodies Available
Useful Reagents Available
Product Specific References
References for IFN Gamma antibody
Wei, Y. et al. (2016) Generation and protective efficacy of a cold-adapted attenuated avian H9N2 influenza vaccine.
Sci Rep. 6: 30382.
Andersen, S.H. et al. (2017) Quantification and phenotypic characterisation of peripheral IFN-γ producing leucocytes in chickens vaccinated against Newcastle disease.
Vet Immunol Immunopathol. 193-194: 18-28.
Staeheli, P. et al. (2001) Cytokines of birds: conserved functions--a largely different look.
J Interferon Cytokine Res. 21 (12): 993-1010.
Weining, K.C. et al. (1996) Biological properties of recombinant chicken interferon-gamma.
Eur J Immunol. 26 (10): 2440-7.
Schultz, U. et al. (1995) Recombinant chicken interferon: a potent antiviral agent that lacks intrinsic macrophage activating factor activity.
Eur J Immunol. 25 (3): 847-51.
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