IFN Gamma antibody

Rabbit anti Chicken Interferon Gamma:Preservative Free

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IFN Gamma

Product Code Applications Pack Size List Price Your Price Qty
AHP945Z
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN WB 0.5 mg loader
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loader
Search for Batch Specific Datasheets

Rabbit anti Chicken interferon gamma antibody recognizes chicken interferon gamma (chIFN-gamma). There are at least three distinct chicken interferon proteins, designated as chIFN-alpha, chIFN-beta and chIFN-gamma.

chIFN-gamma was first identified, when supernatant from chicken spleen cells stimulated with concanavalin A (ConA ), displayed macrophage-activating factor (MAF) activity - the principle function of mammalian type II IFN-gamma (Vonbulow et al. 1984). Cloning of IFN-gamma cDNA from activated chicken T cells indicates that recombinant chicken IFN-gamma activates macrophages, strongly upregulating the expression of MHC class II and iNOS genes (Weining et al. 1996).

Rabbit anti Chicken interferon gamma antibody can be used to neutralize chIFN-gamma activity and to discriminate the antiviral activity of chIFN-gamma from that of chIFN-alpha and chIFN-beta (Weining et al. 1996).

Target Species
Chicken
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to chicken Interferon Gamma were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography on protein A.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present.
Immunogen
Recombinant chicken interferon gamma
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Functional Assays 10ug/ml 50ug/ml
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP945Z detects a band of approximately 18 kDa.

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References for IFN Gamma antibody

  1. Wei, Y. et al. (2016) Generation and protective efficacy of a cold-adapted attenuated avian H9N2 influenza vaccine.
    Sci Rep. 6: 30382.
  2. Andersen, S.H. et al. (2017) Quantification and phenotypic characterisation of peripheral IFN-γ producing leucocytes in chickens vaccinated against Newcastle disease.
    Vet Immunol Immunopathol. 193-194: 18-28.
  3. Wang, M. et al. (2018) Cross- immunity of a H9N2 live attenuated influenza vaccine against H5N2 highly pathogenic avian influenza virus in chickens.
    Vet Microbiol. 220: 57-66.

Further Reading

  1. Staeheli, P. et al. (2001) Cytokines of birds: conserved functions--a largely different look.
    J Interferon Cytokine Res. 21 (12): 993-1010.
  2. Weining, K.C. et al. (1996) Biological properties of recombinant chicken interferon-gamma.
    Eur J Immunol. 26 (10): 2440-7.
  3. Schultz, U. et al. (1995) Recombinant chicken interferon: a potent antiviral agent that lacks intrinsic macrophage activating factor activity.
    Eur J Immunol. 25 (3): 847-51.

Synonyms
Interferon Gamma
RRID
AB_566996
UniProt
P49708
Entrez Gene
IFNG
GO Terms
GO:0009615 response to virus
GO:0005125 cytokine activity
GO:0005133 interferon-gamma receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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