IFN Gamma antibody
Rabbit anti Chicken interferon-gamma antibody (AHP945Z) used for the evaluation of interferon-γ producing T-cells by flow cytometry.
Image caption:
(A) The frequency of IFN-γ+CD4+ or IFN-γ+CD8+ T cells in lungs. Chickens in groups of five were vaccinated with mock vaccine or SD/01/10-ca on day 0 and challenged intranasally with 106 EID50 of A/chicken/Hebei/YT/2010 on day 14 after vaccination. Lungs were harvested on day 7 after challenge. IFN-γ+CD4+ or IFN-γ+CD8+ T cells in lungs stimulated with SD/01/10-wt were measured. Percentages of IFN-γ+CD4+ or IFN-γ+CD8+ T cells within CD4+ or CD8+ T cells were analyzed. *P <0.05, by one-way analysis of variance of the difference between SD/01/10-ca and mock vaccine group on day 7 after challenge. (B) HI antibody responses to homologous and heterologous viruses after vaccination with SD/01/10-ca. Serum samples were collected on day 14 post immunization, and the reactivity of collected sera (n = 3) to SD/01/10-wt or five heterologous H9N2 influenza viruses was assessed by an HI assay. Abbreviations: BJ, Beijing; Ck, chicken; GD, Guangdong; HeB, Hebei; SD, Shandong.
From: Wei Y, Qi L, Gao H, Sun H, Pu J, Sun Y, Liu J.
Generation and protective efficacy of a cold-adapted attenuated avian H9N2 influenza vaccine.
Sci Rep. 2016 Jul 26;6:30382.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rabbit anti Chicken interferon gamma antibody (AHP945) used for the evaluation of intracellular gamma interferon in chicken lymphocytes by flow cytometry.
Image caption:
Intracellular staining of IFN-γ in transfected CHO cells. A) CHO cells were either transfected with plasmids containing the chicken IFN-γ gene or empty plasmids (mock controls). Cells (day 22 after transfection) were cultured with or without BFA for 18 hours and subsequently stained intracellularly using monoclonal anti-chicken IFN-γ antibody (Mab80) conjugated with APC. Representative samples are shown with frequency of IFN-γ+ cells indicated above gate. B) Comparison of transfected CHO cells (day 30 after transfection) cultured with 10 μg/ml BFA for 18 hours and subsequently stained intracellularly with either commercial ELISA capture antibody (5C.123.08) & A647 conjugated secondary anti-mouse IgG1 or monoclonal anti-chicken IFN-γ antibody directly conjugated with APC (Mab80) or commercial ELISA detection antibody (5C.123.02) directly conjugated with APC or polyclonal rabbit anti-chicken IFN-γ antibody & FITC-conjugated secondary goat anti-rabbit IgG. Representative samples are show with frequency of IFN-γ+ cells indicated above gate.
From: Andersen SH, Vervelde L, Sutton K, Norup LR, Wattrang E, Juul-Madsen HR, Dalgaard TS.
Quantification and phenotypic characterisation of peripheral IFN-γ producing leucocytes in chickens vaccinated against Newcastle disease.
Vet Immunol Immunopathol. 2017 Dec;193-194:18-28.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rabbit anti Chicken interferon gamma antibody recognizes chicken interferon gamma (chIFN-gamma). There are at least three distinct chicken interferon proteins, designated as chIFN-alpha, chIFN-beta and chIFN-gamma.
chIFN-gamma was first identified, when supernatant from chicken spleen cells stimulated with concanavalin A (ConA ), displayed macrophage-activating factor (MAF) activity - the principle function of mammalian type II IFN-gamma (Vonbulow et al. 1984). Cloning of IFN-gamma cDNA from activated chicken T cells indicates that recombinant chicken IFN-gamma activates macrophages, strongly upregulating the expression of MHC class II and iNOS genes (Weining et al. 1996).
Rabbit anti Chicken interferon gamma antibody can be used to neutralize chIFN-gamma activity and to discriminate the antiviral activity of chIFN-gamma from that of chIFN-alpha and chIFN-beta (Weining et al. 1996).
chIFN-gamma was first identified, when supernatant from chicken spleen cells stimulated with concanavalin A (ConA ), displayed macrophage-activating factor (MAF) activity - the principle function of mammalian type II IFN-gamma (Vonbulow et al. 1984). Cloning of IFN-gamma cDNA from activated chicken T cells indicates that recombinant chicken IFN-gamma activates macrophages, strongly upregulating the expression of MHC class II and iNOS genes (Weining et al. 1996).
Rabbit anti Chicken interferon gamma antibody can be used to neutralize chIFN-gamma activity and to discriminate the antiviral activity of chIFN-gamma from that of chIFN-alpha and chIFN-beta (Weining et al. 1996).
Product Details
- Target Species
- Chicken
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to chicken Interferon Gamma were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography on protein G.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present.
- Immunogen
- Recombinant chicken interferon gamma
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P49708
- Entrez Gene
- IFNG
- GO Terms
- GO:0009615 response to virus
- GO:0005125 cytokine activity
- GO:0005133 interferon-gamma receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- Regulatory
- For research purposes only
Applications of IFN Gamma antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Functional Assays | 10ug/ml | 50ug/ml | |
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP945Z detects a band of approximately 18 kDa.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for IFN Gamma antibody
-
Wei, Y. et al. (2016) Generation and protective efficacy of a cold-adapted attenuated avian H9N2 influenza vaccine.
Sci Rep. 6: 30382. -
Andersen, S.H. et al. (2017) Quantification and phenotypic characterisation of peripheral IFN-γ producing leucocytes in chickens vaccinated against Newcastle disease.
Vet Immunol Immunopathol. 193-194: 18-28.
Further Reading
-
Staeheli, P. et al. (2001) Cytokines of birds: conserved functions--a largely different look.
J Interferon Cytokine Res. 21 (12): 993-1010. -
Weining, K.C. et al. (1996) Biological properties of recombinant chicken interferon-gamma.
Eur J Immunol. 26 (10): 2440-7. -
Schultz, U. et al. (1995) Recombinant chicken interferon: a potent antiviral agent that lacks intrinsic macrophage activating factor activity.
Eur J Immunol. 25 (3): 847-51.
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