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IFN Alpha antibody

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Rabbit anti Chicken Interferon Alpha:Preservative Free

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP943Z
Datasheet
E FN IP WB
SDS
0.5 mg loader

Rabbit anti Chicken interferon alpha antibody recognizes chicken Interferon alpha (chIFNα). Similarities exist between chicken IFN and mammalian IFNI, revealing the conservation of cysteine residues and the existence of around ten closely related intron-less IFN alpha genes (Sick et al. 1996), chIFN-alpha genes are strongly expressed following viral infection of monocyte-derived macrophage and embryo fibroblasts and acts as a potent antiviral agent (Schwartz et al. 2004).

Rabbit anti Chicken interferon alpha antibody (AHP943Z) cross reacts with IFN beta in western blots but does not show any cross reactivity with IFN gamma and in bioassays, Rabbit anti Chicken interferon alpha antibody neutralizes chIFN-alpha, but not chIFN-beta, and can therefore be used to discriminate IFN alpha and IFN beta bioactivities (Schwartz et al. 2004).

Product Details

Target Species
Chicken
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to chicken Interferon alpha were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography on protein G.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant chicken IFNα (PAP004)
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P42165
Entrez Gene
IFNA3
GO Terms
GO:0006952 defense response
GO:0009615 response to virus
GO:0005125 cytokine activity
GO:0005126 cytokine receptor binding
GO:0005615 extracellular space
Regulatory
For research purposes only

Applications of IFN Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Functional Assays
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP943Z detects a band of approximately 19kDa.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

References for IFN Alpha antibody

  1. Schwarz, H. et al. (2004) Synthesis of IFN-beta by virus-infected chicken embryo cells demonstrated with specific antisera and a new bioassay.
    J Interferon Cytokine Res. 24 (3): 179-84.

Further Reading

  1. Staeheli, P. et al. (2001) Cytokines of birds: conserved functions--a largely different look.
    J Interferon Cytokine Res. 21 (12): 993-1010.
  2. Sick, C. et al. (1996) A family of genes coding for two serologically distinct chicken interferons.
    J Biol Chem. 271 (13): 7635-9.
  3. Schultz, U. et al. (1995) Recombinant chicken interferon: a potent antiviral agent that lacks intrinsic macrophage activating factor activity.
    Eur J Immunol. 25 (3): 847-51.

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