IFN Alpha antibody

Rabbit anti Chicken interferon alpha antibody (AHP943Z) cross reacts with IFN beta in western blots but does not show any cross reactivity with IFN gamma and in bioassays, Rabbit anti Chicken interferon alpha antibody neutralizes chIFN-alpha, but not chIFN-beta, and can therefore be used to discriminate IFN alpha and IFN beta bioactivities (Schwartz et al. 2004).
Product Details
- Target Species
- Chicken
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to chicken Interferon alpha were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography on protein G.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant chicken IFNα (PAP004)
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P42165
- Entrez Gene
- IFNA3
- GO Terms
- GO:0006952 defense response
- GO:0009615 response to virus
- GO:0005125 cytokine activity
- GO:0005126 cytokine receptor binding
- GO:0005615 extracellular space
- Regulatory
- For research purposes only
Applications of IFN Alpha antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | |||
Functional Assays | |||
Immunoprecipitation | |||
Western Blotting |
- Western Blotting
- AHP943Z detects a band of approximately 19kDa.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for IFN Alpha antibody
-
Schwarz, H. et al. (2004) Synthesis of IFN-beta by virus-infected chicken embryo cells demonstrated with specific antisera and a new bioassay.
J Interferon Cytokine Res. 24 (3): 179-84.
Further Reading
-
Staeheli, P. et al. (2001) Cytokines of birds: conserved functions--a largely different look.
J Interferon Cytokine Res. 21 (12): 993-1010. -
Sick, C. et al. (1996) A family of genes coding for two serologically distinct chicken interferons.
J Biol Chem. 271 (13): 7635-9. -
Schultz, U. et al. (1995) Recombinant chicken interferon: a potent antiviral agent that lacks intrinsic macrophage activating factor activity.
Eur J Immunol. 25 (3): 847-51.
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