Rabbit anti Chicken interferon alpha antibody
recognizes chicken Interferon alpha (chIFNα). Similarities exist between chicken IFN and mammalian IFNI, revealing the conservation of cysteine residues and the existence of around ten closely related intron-less IFN alpha genes (Sick et al. 1996
), chIFN-alpha genes are strongly expressed following viral infection of monocyte-derived macrophage and embryo fibroblasts and acts as a potent antiviral agent (Schwartz et al. 2004
Rabbit anti Chicken interferon alpha antibody (AHP943Z
) cross reacts with IFN beta in western blots but does not show any cross reactivity with IFN gamma and in bioassays, Rabbit anti Chicken interferon alpha antibody neutralizes chIFN-alpha, but not chIFN-beta, and can therefore be used to discriminate IFN alpha and IFN beta bioactivities (Schwartz et al. 2004
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to chicken Interferon alpha were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography on protein G.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Recombinant chicken IFNα (PAP004)
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- Entrez Gene
- GO Terms
response to virus
cytokine receptor binding
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IFN Alpha antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP943Z detects a band of approximately 19kDa.
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Secondary Antibodies Available
Useful Reagents Available