IFN Alpha antibody
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Rabbit anti Chicken Interferon Alpha:Preservative Free
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IFN Alpha
Antibody Quality and Performance Guarantee
| Rabbit anti Chicken interferon alpha antibody recognizes chicken Interferon alpha (chIFNα). Similarities exist between chicken IFN and mammalian IFNI, revealing the conservation of cysteine residues and the existence of around ten closely related intron-less IFN alpha genes (Sick et al. 1996), chIFN-alpha genes are strongly expressed following viral infection of monocyte-derived macrophage and embryo fibroblasts and acts as a potent antiviral agent (Schwartz et al. 2004). Rabbit anti Chicken interferon alpha antibody (AHP943Z) cross reacts with IFN beta in western blots but does not show any cross reactivity with IFN gamma and in bioassays, Rabbit anti Chicken interferon alpha antibody neutralizes chIFN-alpha, but not chIFN-beta, and can therefore be used to discriminate IFN alpha and IFN beta bioactivities (Schwartz et al. 2004). |
- Target Species
- Chicken
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to chicken Interferon alpha were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography on protein A.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant chicken IFNα (PAP004)
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
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| Functional Assays | |
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| Immunoprecipitation | |
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| Western Blotting | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP943Z detects a band of approximately 19kDa.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| List Price | Your Price | ||||||
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Sheep anti Rabbit IgG:FITC | ||||||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
References for IFN Alpha antibody
-
Schwarz, H. et al. (2004) Synthesis of IFN-beta by virus-infected chicken embryo cells demonstrated with specific antisera and a new bioassay.
J Interferon Cytokine Res. 24 (3): 179-84.
Further Reading
-
Staeheli, P. et al. (2001) Cytokines of birds: conserved functions--a largely different look.
J Interferon Cytokine Res. 21 (12): 993-1010. -
Sick, C. et al. (1996) A family of genes coding for two serologically distinct chicken interferons.
J Biol Chem. 271 (13): 7635-9. -
Schultz, U. et al. (1995) Recombinant chicken interferon: a potent antiviral agent that lacks intrinsic macrophage activating factor activity.
Eur J Immunol. 25 (3): 847-51.
- Synonyms
- Interferon Alpha
- RRID
- AB_2122227
- UniProt
- P42165
- Entrez Gene
- IFNA3
- GO Terms
- GO:0006952 defense response
- GO:0009615 response to virus
- GO:0005125 cytokine activity
- GO:0005126 cytokine receptor binding
- GO:0005615 extracellular space
AHP943Z
156597
161110
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