Salmonella Group Antigen antibody

Rabbit anti Salmonella Group Antigen

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Salmonella Group Antigen

Product Code Applications Pack Size List Price Your Price Qty
8209-4006
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IF 1 ml loader
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Rabbit anti Salmonella group antigen antibody recognizes a Salmonella group antigen. Salmonella is a genus of the family Enterobacteriaceae populated by a variety of Gram negative rod-shaped bacteria, many of which are pathogenic and cause a range of diseases in humans. Salmonellae possess 3 major surface antigens: the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria. This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A.

Rabbit anti Salmonella group antigen antibody is polyvalent for Salmonella O and H antigens.

Rabbit anti Salmonella group antigen antibody is unabsorbed and may cross react with related Enterobacteriaceae.

Target Species
Bacterial
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Mixture of Salmonella enteriditis, S. typhimurium and S. heidelburg.
Approx. Protein Concentrations
IgG concentration 4.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

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References for Salmonella Group Antigen antibody

  1. Duffy, G. et al. (2000) A membrane-immunofluorescent-viability staining technique for the detection of Salmonella spp. from fresh and processed meat samples.
    J Appl Microbiol. 89 (4): 587-94.
  2. Hunter, D.M. et al. (2010) Rapid detection and identification of bacterial pathogens by using an ATP bioluminescence immunoassay.
    J Food Prot. 73: 739-46.
  3. Ewald, M. et al. (2015) A multi-analyte biosensor for the simultaneous label-free detection of pathogens and biomarkers in point-of-need animal testing.
    Anal Bioanal Chem. 407 (14): 4005-13.
  4. Ewald M et al. (2013) A robust sensor platform for label-free detection of anti-Salmonella antibodies using undiluted animal sera.
    Anal Bioanal Chem. 405 (20): 6461-9.
  5. Cloak, O.M. et al. (1999) Isolation and detection of Listeria spp, Salmonella spp and Yersinia spp using a simultaneous enrichment step followed by a surface adhesion immunofluorescent technique.
    J Microbiol Methods. 39 (1): 33-43.
  6. de Souza, S.O. et al. (2014) Osteomyelitis caused by Salmonella enterica serovar derby in boa constrictor.
    J Zoo Wildl Med. 45 (3): 642-4.
  7. Tian B et al. (2015) Blu-ray optomagnetic measurement based competitive immunoassay for Salmonella detection.
    Biosens Bioelectron. 77: 32-39.
  8. Tsougeni, K. et al. (2016) Plasma nanotextured polymeric lab-on-a-chip for highly efficient bacteria capture and lysis.
    Lab Chip. 16 (1): 120-31.
  9. View The Latest Product References
  10. Le, U.N. et al. (2011) Engineering and visualization of bacteria for targeting infarcted myocardium.
    Mol Ther. 19 (5): 951-9.
  11. Volpe, G. et al. (2016) Development and evaluation of an ELIME assay to reveal the presence of Salmonella in irrigation water: Comparison with Real-Time PCR and the Standard Culture Method.
    Talanta. 149: 202-10.
  12. Cruz-Adalia, A. et al. (2016) T Cells Capture Bacteria by Transinfection from Dendritic Cells.
    J Vis Exp. (107): e52976.
  13. Tian, B. et al. (2016) Multi-scale magnetic nanoparticle based optomagnetic bioassay for sensitive DNA and bacteria detection
    Analytical Methods. 8 (25): 5009-16.
  14. Kastania, A. et al. (2017) Binding kinetics of bacteria cells on immobilized antibodies in microfluidic channels: Modeling and experiments
    Sensors and Actuators B: Chemical. 253: 247-57.
  15. Farka, Z. et al. (2018) Prussian Blue Nanoparticles as a Catalytic Label in a Sandwich Nanozyme-Linked Immunosorbent Assay.
    Anal Chem. 90 (3): 2348-54.
  16. Schenk, F. et al. (2018) Development of a paper-based lateral flow immunoassay for simultaneous detection of lipopolysaccharides of Salmonella serovars.
    Anal Bioanal Chem. 410 (3): 863-8.
  17. Kim, D.K. et al. (2014) Inverse agonist of estrogen-related receptor γ controls Salmonella typhimurium infection by modulating host iron homeostasis.
    Nat Med. 20 (4): 419-24.
  18. Tsougeni, K. et al. (2019) A modular integrated lab-on-a-chip platform for fast and highly efficient sample preparation for foodborne pathogen screening
    Sensors and Actuators B: Chemical. 288: 171-9.
  19. Angelopoulou, M. et al. (2021) Rapid Detection of Salmonella typhimurium in Drinking Water by a White Light Reflectance Spectroscopy Immunosensor.
    Sensors (Basel). 21 (8): 2683.
  20. Tsougeni, K. et al. (2019) A modular integrated lab-on-a-chip platform for fast and highly efficient sample preparation for foodborne pathogen screening
    Sensors and Actuators B: Chemical. 288: 171-9.

Immunofluorescence

RRID
AB_619545
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