Salmonella Group Antigen antibody

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Rabbit anti Salmonella Group Antigen:Biotin

Rabbit anti Salmonella Group Antigen:HRP

Rabbit anti Salmonella Group Antigen

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
8209-4011 IF 1 ml
8209-4021 E 1 ml
8209-4006 IF 1 ml
Rabbit anti Salmonella group antigen antibody recognizes a Salmonella group antigen. Salmonella is a genus of the family Enterobacteriaceae populated by a variety of Gram negative rod-shaped bacteria, many of which are pathogenic and cause a range of diseases in humans. Salmonellae possess 3 major surface antigens: the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria. This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A.

Rabbit anti Salmonella group antigen antibody is polyvalent for Salmonella O and H antigens.

Rabbit anti Salmonella group antigen antibody is unabsorbed and may cross react with related Enterobacteriaceae.

Product Details

Target Species
Bacterial
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Preservative Stabilisers
0.002% Thiomersal
1% Bovine Serum Albumin
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Mixture of Salmonella enteriditis, S. typhimurium and S. heidelburg.
Approx. Protein Concentrations
IgG concentration 4 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 4.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Salmonella Group Antigen antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1/1000
ELISA 1/200 1/1000
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Application Based External Images

Immunofluorescence

Product Specific References

References for Salmonella Group Antigen antibody

  1. Duffy, G. et al. (2000) A membrane-immunofluorescent-viability staining technique for the detection of Salmonella spp. from fresh and processed meat samples.
    J Appl Microbiol. 89 (4): 587-94.
  2. Hunter, D.M. et al. (2010) Rapid detection and identification of bacterial pathogens by using an ATP bioluminescence immunoassay.
    J Food Prot. 73: 739-46.
  3. Ewald, M. et al. (2015) A multi-analyte biosensor for the simultaneous label-free detection of pathogens and biomarkers in point-of-need animal testing.
    Anal Bioanal Chem. 407 (14): 4005-13.
  4. Ewald M et al. (2013) A robust sensor platform for label-free detection of anti-Salmonella antibodies using undiluted animal sera.
    Anal Bioanal Chem. 405 (20): 6461-9.
  5. Cloak, O.M. et al. (1999) Isolation and detection of Listeria spp, Salmonella spp and Yersinia spp using a simultaneous enrichment step followed by a surface adhesion immunofluorescent technique.
    J Microbiol Methods. 39 (1): 33-43.
  6. de Souza, S.O. et al. (2014) Osteomyelitis caused by Salmonella enterica serovar derby in boa constrictor.
    J Zoo Wildl Med. 45 (3): 642-4.
  7. Tian B et al. (2015) Blu-ray optomagnetic measurement based competitive immunoassay for Salmonella detection.
    Biosens Bioelectron. 77: 32-39.
  8. Tsougeni, K. et al. (2016) Plasma nanotextured polymeric lab-on-a-chip for highly efficient bacteria capture and lysis.
    Lab Chip. 16 (1): 120-31.
  9. Le, U.N. et al. (2011) Engineering and visualization of bacteria for targeting infarcted myocardium.
    Mol Ther. 19 (5): 951-9.
  10. Volpe, G. et al. (2016) Development and evaluation of an ELIME assay to reveal the presence of Salmonella in irrigation water: Comparison with Real-Time PCR and the Standard Culture Method.
    Talanta. 149: 202-10.
  11. Cruz-Adalia, A. et al. (2016) T Cells Capture Bacteria by Transinfection from Dendritic Cells.
    J Vis Exp. (107): e52976.
  12. Tian, B. et al. (2016) Multi-scale magnetic nanoparticle based optomagnetic bioassay for sensitive DNA and bacteria detection.
    Anal Methods. Jun 3 [Epub ahead of print]
  13. Kastania, A. et al. (2017) Binding kinetics of bacteria cells on immobilized antibodies in microfluidic channels: Modeling and experiments
    Sensors and Actuators B: Chemical. 253: 247-57.
  14. Farka, Z. et al. (2018) Prussian Blue Nanoparticles as a Catalytic Label in a Sandwich Nanozyme-Linked Immunosorbent Assay.
    Anal Chem. Jan 18 [Epub ahead of print].
  15. Schenk, F. et al. (2018) Development of a paper-based lateral flow immunoassay for simultaneous detection of lipopolysaccharides of Salmonella serovars.
    Anal Bioanal Chem. 410 (3): 863-8.