Listeria monocytogenes antibody

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Rabbit anti Listeria monocytogenes Kc 1778+ H 2b

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
5688-1990
Datasheet
E P*
SDS
1 ml loader

Rabbit anti Listeria monocytogenes antibody reacts with Listeria monocytogenes whole cells. Rabbit anti Listeria monocytogenes antibody has not been absorbed and may react with related micro-organisms.

Rabbit anti Listeria monocytogenes antibody has been used successfully for the demonstration of internalized L. monocytogenes in formalin fixed peripheral blood mononuclear cells by immunofluorescence (Salem et al. 2015).

Product Details

Target Species
Bacterial
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Listeria monocytogenes. whole cells.
Approx. Protein Concentrations
4.0 mg/ml

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of Listeria monocytogenes antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Paraffin 1
  1. 1This product requires protein digestion pre-treatment of paraffin sections e.g. pronase.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
100x Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml

Application Based External Images

Flow Cytometry

Immunofluorescence

Product Specific References

References for Listeria monocytogenes antibody

  1. Marusov, G. et al. (2012) A microarray biosensor for multiplexed detection of microbes using grating-coupled surface plasmon resonance imaging.
    Environ Sci Technol. 46 (1): 348-59.
  2. Salem M et al. (2015) Species-specific engagement of human nucleotide oligomerization domain 2 (NOD)2 and Toll-like receptor (TLR) signalling upon intracellular bacterial infection: role of Crohn's associated NOD2 gene variants.
    Clin Exp Immunol. 179 (3): 426-34.
  3. Témoin, S. et al. (2008) Multiple point mutations in virulence genes explain the low virulence of Listeria monocytogenes field strains.
    Microbiology. 154 (Pt 3): 939-48.
  4. Cruz-Adalia, A. et al. (2017) Conventional CD4+ T cells present bacterial antigens to induce cytotoxic and memory CD8+ T cell responses.
    Nat Commun. 8 (1): 1591.
  5. Okita, Y. et al. (2016) Interleukin-22-Induced Antimicrobial Phospholipase A2 Group IIA Mediates Protective Innate Immunity of Nonhematopoietic Cells against Listeria monocytogenes.
    Infect Immun. 84 (2): 573-9.

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