Listeria monocytogenes antibody
Rabbit anti Listeria monocytogenes antibody (5688-1990) used to stain Listeria monocytogenes in CD4+ T cells by immunofluorescence.
Image caption:
Orthogonal views of Listeria tpCD4+ T cells purified at 3 h of conjugate formation. Actin is shown in magenta, and Listeria in green. XY together with YZ and XZ views (from lines marked in XY) are shown. Bar = 10μm.
From: Cruz-Adalia A, Ramirez-Santiago G, Osuna-Pérez J, Torres-Torresano M, Zorita V, Martínez-Riaño A, Boccasavia V, Borroto A, Martínez Del Hoyo G, Gozález-Granado JM, Alarcón B, Sánchez-Madrid F, Veiga E.
Conventional CD4(+) T cells present bacterial antigens to induce cytotoxic and memory CD8(+) T cell responses.
Nat Commun. 2017 Nov 17;8(1):1591.
Rabbit anti Listeria monocytogenes:Biotin
Rabbit anti Listeria monocytogenes ATCC 43251:FITC
Rabbit anti Listeria monocytogenes ATCC 43251:HRP
Rabbit anti Listeria monocytogenes ATCC 43251
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| 5688-1992 | E | 1 ml | ||||
| 5688-1995 | IF | 1 ml | ||||
| 5688-2000 | C E IF WB | 1 ml | ||||
| 5688-1990 | E P* | 1 ml |
Rabbit anti Listeria monocytogenes ATCC 43251 antibody reacts with Listeria monocytogenes whole cells. Rabbit anti Listeria monocytogenes ATCC 43251 antibody has not been absorbed and may react with related micro-organisms.
Rabbit anti Listeria monocytogenes ATCC 43251 antibody has been used successfully for the demonstration of internalized L. monocytogenes in formalin fixed peripheral blood mononuclear cells by immunofluorescence (Salem et al. 2015).
Rabbit anti Listeria monocytogenes ATCC 43251 antibody has been used successfully for the demonstration of internalized L. monocytogenes in formalin fixed peripheral blood mononuclear cells by immunofluorescence (Salem et al. 2015).
Product Details
- Target Species
- Bacterial
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified Ig conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified Ig conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
10 mg/ml Bovine Serum Albumin - Preservative Stabilisers
- 0.002% Thiomersal
10 mg/ml Bovine Serum Albumin - Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- Listeria monocytogenes. whole cells. ATCC strain 43251.
- Approx. Protein Concentrations
- IgG concentration 4.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 4.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Approx. Protein Concentrations
- 4.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of Listeria monocytogenes antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Immunofluorescence | 1/10 | 1/50 | |
| ELISA | 1/200 | 1/1000 | |
| Immunofluorescence | |||
| Immunohistology - Frozen | 1/20 | 1/200 | |
| Western Blotting | 1/20 | 1/200 | |
| ELISA | |||
| Immunohistology - Paraffin 1 |
- 1This product requires protein digestion pre-treatment of paraffin sections e.g. pronase.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
- Immunohistology
- Acetone is recommended.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | 100 ml | C E P WB | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | 500 ml | C E P WB | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | 1000 ml | C E P WB | ||
| TMB Signal+ | BUF054A | 100 ml | E | ||
| TMB Core | BUF056A | 100 ml | E | ||
| TMB Core+ | BUF062A | 100 ml | E | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025A | 500 ml | P | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025C | 50 ml | P |
Product Specific References
References for Listeria monocytogenes antibody
-
Marusov, G. et al. (2012) A microarray biosensor for multiplexed detection of microbes using grating-coupled surface plasmon resonance imaging.
Environ Sci Technol. 46 (1): 348-59. -
Salem M et al. (2015) Species-specific engagement of human nucleotide oligomerization domain 2 (NOD)2 and Toll-like receptor (TLR) signalling upon intracellular bacterial infection: role of Crohn's associated NOD2 gene variants.
Clin Exp Immunol. 179 (3): 426-34. -
Témoin, S. et al. (2008) Multiple point mutations in virulence genes explain the low virulence of Listeria monocytogenes field strains.
Microbiology. 154 (Pt 3): 939-48. -
Cruz-Adalia, A. et al. (2017) Conventional CD4+ T cells present bacterial antigens to induce cytotoxic and memory CD8+ T cell responses.
Nat Commun. 8 (1): 1591. -
Okita, Y. et al. (2016) Interleukin-22-Induced Antimicrobial Phospholipase A2 Group IIA Mediates Protective Innate Immunity of Nonhematopoietic Cells against Listeria monocytogenes.
Infect Immun. 84 (2): 573-9.
