Listeria monocytogenes antibody
Rabbit anti Listeria monocytogenes antibody (5688-1990) used to stain Listeria monocytogenes in CD4+ T cells by immunofluorescence.
Image caption:
Orthogonal views of Listeria tpCD4+ T cells purified at 3 h of conjugate formation. Actin is shown in magenta, and Listeria in green. XY together with YZ and XZ views (from lines marked in XY) are shown. Bar = 10μm.
From: Cruz-Adalia A, Ramirez-Santiago G, Osuna-Pérez J, Torres-Torresano M, Zorita V, Martínez-Riaño A, Boccasavia V, Borroto A, Martínez Del Hoyo G, Gozález-Granado JM, Alarcón B, Sánchez-Madrid F, Veiga E.
Conventional CD4(+) T cells present bacterial antigens to induce cytotoxic and memory CD8(+) T cell responses.
Nat Commun. 2017 Nov 17;8(1):1591.
Rabbit anti Listeria monocytogenes Kc 1778+ H 2b
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Listeria monocytogenes
Antibody Quality and Performance Guarantee
| Rabbit anti Listeria monocytogenes antibody reacts with Listeria monocytogenes whole cells. Rabbit anti Listeria monocytogenes antibody has not been absorbed and may react with related micro-organisms. Rabbit anti Listeria monocytogenes antibody has been used successfully for the demonstration of internalized L. monocytogenes in formalin fixed peripheral blood mononuclear cells by immunofluorescence (Salem et al. 2015). |
- Target Species
- Bacterial
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- Listeria monocytogenes. whole cells.
- Approx. Protein Concentrations
- IgG concentration 5.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Immunohistology - Paraffin 1 | |
- 1This product requires protein digestion pre-treatment of paraffin sections e.g. pronase.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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References for Listeria monocytogenes antibody
-
Témoin, S. et al. (2008) Multiple point mutations in virulence genes explain the low virulence of Listeria monocytogenes field strains.
Microbiology. 154 (Pt 3): 939-48. -
Marusov, G. et al. (2012) A microarray biosensor for multiplexed detection of microbes using grating-coupled surface plasmon resonance imaging.
Environ Sci Technol. 46 (1): 348-59. -
Salem M et al. (2015) Species-specific engagement of human nucleotide oligomerization domain 2 (NOD)2 and Toll-like receptor (TLR) signalling upon intracellular bacterial infection: role of Crohn's associated NOD2 gene variants.
Clin Exp Immunol. 179 (3): 426-34. -
Okita, Y. et al. (2016) Interleukin-22-Induced Antimicrobial Phospholipase A2 Group IIA Mediates Protective Innate Immunity of Nonhematopoietic Cells against Listeria monocytogenes.
Infect Immun. 84 (2): 573-9. -
Cruz-Adalia, A. et al. (2017) Conventional CD4+ T cells present bacterial antigens to induce cytotoxic and memory CD8+ T cell responses.
Nat Commun. 8 (1): 1591. -
García-Ferreras, R. et al. (2023) Bacteria-instructed B cells cross-prime naïve CD8(+) T cells triggering effective cytotoxic responses.
EMBO Rep. 24 (7): e56131.
- RRID
- AB_620691
5688-1990
151444
155299
156141
159516
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