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Listeria monocytogenes antibody

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Anti Listeria monocytogenes ATCC 43251 Antibody gallery image 1
Published customer image:
Rabbit anti Listeria monocytogenes antibody (5688-1990) used to stain Listeria monocytogenes in CD4+ T cells by immunofluorescence.
Image caption:
Orthogonal views of Listeria tpCD4+ T cells purified at 3 h of conjugate formation. Actin is shown in magenta, and Listeria in green. XY together with YZ and XZ views (from lines marked in XY) are shown. Bar = 10μm.

From: Cruz-Adalia A, Ramirez-Santiago G, Osuna-Pérez J, Torres-Torresano M, Zorita V, Martínez-Riaño A, Boccasavia V, Borroto A, Martínez Del Hoyo G, Gozález-Granado JM, Alarcón B, Sánchez-Madrid F, Veiga E.
Conventional CD4(+) T cells present bacterial antigens to induce cytotoxic and memory CD8(+) T cell responses.
Nat Commun. 2017 Nov 17;8(1):1591.

Rabbit anti Listeria monocytogenes:Biotin

Rabbit anti Listeria monocytogenes ATCC 43251:FITC

Rabbit anti Listeria monocytogenes ATCC 43251:HRP

Rabbit anti Listeria monocytogenes ATCC 43251

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
5688-1992
Datasheet
E
MSDS
1 ml loader

5688-1995
Datasheet
IF
MSDS
1 ml loader

5688-2000
Datasheet
C E IF WB
MSDS
1 ml loader

5688-1990
Datasheet
E P*
MSDS
1 ml loader

Rabbit anti Listeria monocytogenes ATCC 43251 antibody reacts with Listeria monocytogenes whole cells. Rabbit anti Listeria monocytogenes ATCC 43251 antibody has not been absorbed and may react with related micro-organisms.

Rabbit anti Listeria monocytogenes ATCC 43251 antibody has been used successfully for the demonstration of internalized L. monocytogenes in formalin fixed peripheral blood mononuclear cells by immunofluorescence (Salem et al. 2015).

Product Details

Target Species
Bacterial
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified Ig conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified Ig conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
10 mg/ml Bovine Serum Albumin
Preservative Stabilisers
0.002% Thiomersal
10 mg/ml Bovine Serum Albumin
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Listeria monocytogenes. whole cells. ATCC strain 43251.
Approx. Protein Concentrations
IgG concentration 4.0 mg/ml
Approx. Protein Concentrations
IgG concentration 4.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Approx. Protein Concentrations
4.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of Listeria monocytogenes antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence 1/10 1/50
ELISA 1/200 1/1000
Immunofluorescence
Immunohistology - Frozen 1/20 1/200
Western Blotting 1/20 1/200
ELISA
Immunohistology - Paraffin 1
  1. 1This product requires protein digestion pre-treatment of paraffin sections e.g. pronase.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Immunohistology
Acetone is recommended.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
TMB Signal+ BUF054A E 100 ml
TMB Core BUF056A E 100 ml
TMB Core+ BUF062A E 100 ml
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml

Application Based External Images

Flow Cytometry

Immunofluorescence

Product Specific References

References for Listeria monocytogenes antibody

  1. Marusov, G. et al. (2012) A microarray biosensor for multiplexed detection of microbes using grating-coupled surface plasmon resonance imaging.
    Environ Sci Technol. 46 (1): 348-59.
  2. Salem M et al. (2015) Species-specific engagement of human nucleotide oligomerization domain 2 (NOD)2 and Toll-like receptor (TLR) signalling upon intracellular bacterial infection: role of Crohn's associated NOD2 gene variants.
    Clin Exp Immunol. 179 (3): 426-34.
  3. Témoin, S. et al. (2008) Multiple point mutations in virulence genes explain the low virulence of Listeria monocytogenes field strains.
    Microbiology. 154 (Pt 3): 939-48.
  4. Cruz-Adalia, A. et al. (2017) Conventional CD4+ T cells present bacterial antigens to induce cytotoxic and memory CD8+ T cell responses.
    Nat Commun. 8 (1): 1591.
  5. Okita, Y. et al. (2016) Interleukin-22-Induced Antimicrobial Phospholipase A2 Group IIA Mediates Protective Innate Immunity of Nonhematopoietic Cells against Listeria monocytogenes.
    Infect Immun. 84 (2): 573-9.
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