Borrelia burgdorferi antibody
Rabbit anti Borrelia burgdorferi antibody used for the detection of B. burgdorferi in canine myocardium by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Microscopic examination of heart specimens. A- degeneration of myocardium with disturbances in cardiomyocyte structure, presence of fibrous tissue and mild inflammatory infiltrates (arrows) (H&E; 200x); B-severe cardiomyocyte degeneration accompanied by severe granulocytic and slight lymph o-plasmocytic infiltration in heart tissue adjutant to embolic material (H&E; 200x); C-severe granulocytic and moderate lympho-plasmocytic infiltration of infarct site (H&E; 200x); D-vegetative and spore forms of Borrelia burgdorferi in heart specimens (IHC stain, 600x); E-Borrelia burgdorferi spore forms in heart specimens (IHC stain, 600x).
From: Janus, I. et al. Myocarditis in dogs: etiology, clinical and histopathological features. 11 cases (2007-2013). Irish Vet J. 2010 67:28.
Rabbit anti Borrelia burgdorferi recognizes one of the causative agents of Lyme disease. B. burgdorferi is a spirochaete bacterium and one of the few pathogenic bacteria capable of surviving in the absence of iron.
This antibody cross reacts with Treponema pallidum, Borrelia hermsii and Borrelia parkeri.
This antibody cross reacts with Treponema pallidum, Borrelia hermsii and Borrelia parkeri.
Product Details
- Target Species
- Bacterial
- Product Form
- Purified Ig conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- Whole cell preparation from B. burgdorferi.
- Approx. Protein Concentrations
- IgG concentration 4 mg/ml
- Approx. Protein Concentrations
- 4 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of Borrelia burgdorferi antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence 1 | 1/10 | 1/50 | |
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |||
| Western Blotting |
- 1Acetone fixation is recommended for best results.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | 500 ml | P | ||
| Antigen Retrieval Buffer, pH8.0 | BUF025C | 50 ml | P | ||
| TidyBlot™ Western Blot Detection Reagent:HRP | STAR209P | 0.5 ml | WB* |
Product Specific References
References for Borrelia burgdorferi antibody
-
De Socio, G.V. et al. (2011) Malignant syphilis with ocular involvement in an HIV-infected patient.
Int J STD AIDS. 22: 298-300. -
Drago, F. et al. (2015) Primary syphilis of the oropharynx: an unusual location of a chancre.
Int J STD AIDS. 26 (9): 679-81. -
Drago, F. et al. (2012) Luetic lymphadenopathy despite negative serology
Int J STD AIDS. 23: 601-2. -
Haedersdal, M. & Weismann, K. (2000) Syphilitic chancre despite use of condoms: "condom chancre".
Acta Derm Venereol. 80 (3): 235-6. -
Janus, I. et al. (2014) Myocarditis in dogs: etiology, clinical and histopathological features (11 cases: 2007-2013).
Ir Vet J. 67 (1): 28. -
Briciu, V.T. et al. (2016) Immunohistochemistry and real-time PCR as diagnostic tools for detection of Borrelia burgdorferi sensu lato in ticks collected from humans.
Exp Appl Acarol. 69 (1): 49-60.
