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Borrelia burgdorferi antibody

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Anti Borrelia burgdorferi Antibody gallery image 1
Published customer image:
Rabbit anti Borrelia burgdorferi antibody (1439-9406) used for the detection of B. burgdorferi in canine myocardium by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Microscopic examination of heart specimens. A- degeneration of myocardium with disturbances in cardiomyocyte structure, presence of fibrous tissue and mild inflammatory infiltrates (arrows) (H&E; 200x); B-severe cardiomyocyte degeneration accompanied by severe granulocytic and slight lymph o-plasmocytic infiltration in heart tissue adjutant to embolic material (H&E; 200x); C-severe granulocytic and moderate lympho-plasmocytic infiltration of infarct site (H&E; 200x); D-vegetative and spore forms of Borrelia burgdorferi in heart specimens (IHC stain, 600x); E-Borrelia burgdorferi spore forms in heart specimens (IHC stain, 600x).

From: Janus, I. et al. Myocarditis in dogs: etiology, clinical and histopathological features. 11 cases (2007-2013).
Irish Vet J. 2010 67:28.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Rabbit anti Borrelia burgdorferi:FITC

Rabbit anti Borrelia burgdorferi

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
1439-9446
Datasheet
IF*
SDS
1 ml loader

1439-9406
Datasheet
C IF P WB
SDS
1 ml loader

Rabbit anti Borrelia burgdorferi recognizes one of the causative agents of Lyme disease. B. burgdorferi is a spirochaete bacterium and one of the few pathogenic bacteria capable of surviving in the absence of iron.

This antibody cross reacts with Treponema pallidum, Borrelia hermsii and Borrelia parkeri.

Product Details

Target Species
Bacterial
Product Form
Purified Ig conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Whole cell preparation from B. burgdorferi.
Approx. Protein Concentrations
IgG concentration 4 mg/ml
Approx. Protein Concentrations
4 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of Borrelia burgdorferi antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1 1/10 1/50
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
  1. 1Acetone fixation is recommended for best results.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

References for Borrelia burgdorferi antibody

  1. De Socio, G.V. et al. (2011) Malignant syphilis with ocular involvement in an HIV-infected patient.
    Int J STD AIDS. 22: 298-300.
  2. Drago, F. et al. (2015) Primary syphilis of the oropharynx: an unusual location of a chancre.
    Int J STD AIDS. 26 (9): 679-81.
  3. Drago, F. et al. (2012) Luetic lymphadenopathy despite negative serology
    Int J STD AIDS. 23: 601-2.
  4. Haedersdal, M. & Weismann, K. (2000) Syphilitic chancre despite use of condoms: "condom chancre".
    Acta Derm Venereol. 80 (3): 235-6.
  5. Janus, I. et al. (2014) Myocarditis in dogs: etiology, clinical and histopathological features (11 cases: 2007-2013).
    Ir Vet J. 67 (1): 28.
  6. Briciu, V.T. et al. (2016) Immunohistochemistry and real-time PCR as diagnostic tools for detection of Borrelia burgdorferi sensu lato in ticks collected from humans.
    Exp Appl Acarol. 69 (1): 49-60.

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