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Arg-Vasopressin antibody

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Anti Arginine Vasopressin Antibody thumbnail image 1 Anti Arginine Vasopressin Antibody thumbnail image 2
Anti Arginine Vasopressin Antibody gallery image 1
Published customer image:
Rabbit anti arginine vasopressin antibody (AHP372) used to reveal arg-vasopressin expressing cells in mouse brain by immunofluorescence.
Image caption:
Immunofluorescence analysis of arginine vasopressin (AVP), glial fibrillary acidic protein (GFAP) of astrocytes and DAPI counterstain of nuclei in the hypothalamus of aged progranulin deficient (Grn-/-) and control mice (Grn+/+)
The Gensat images in the right give an overview of the localization of the paraventricular nucleus (PVN) and the nucleus supraopticus (SON), which are the major sites for vasopressin producing neurons. The panels show AVP, GFAP and DAPI in the PVN as overview and zoom-in images, AVP immunofluorescence was more intense in Grn-/- and AVP positive neurons appear to be enlarged. GFAP staining reveals astrogliosis in Grn-/-. Scale bars 300 μm for overviews and 100 μm for zoom-in images.

From: Hardt S, Valek L, Zeng-Brouwers J, Wilken-Schmitz A, Schaefer L, Tegeder I.
Progranulin Deficient Mice Develop Nephrogenic Diabetes Insipidus.
Aging Dis. 2018 Oct 1;9(5):817-830.
doi: 10.14336/AD.2017.1127.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Arginine Vasopressin Antibody gallery image 2
Published customer image:
Rabbit anti arginine vasopressin antibody (AHP372) used to reveal arg-vasopressin expressing cells in mouse brain by immunofluorescence.
Image caption:
Immunofluorescence analysis of arginine vasopressin (AVP), glial fibrillary acidic protein (GFAP) of astrocytes and DAPI counterstain of nuclei in the nucleus supraopticus (SON) of the hypothalamus of aged progranulin deficient (Grn-/-) and control mice (Grn+/+).
The panels show AVP, GFAP and DAPI in the PVN as overview and zoom-in images, AVP immunofluorescence was more intense in Grn-/- and AVP positive neurons appear to be enlarged. GFAP staining reveals astrogliosis in Grn-/-. Scale bars 300 μm for overviews and 100 μm for zoom-in images.

From: Hardt S, Valek L, Zeng-Brouwers J, Wilken-Schmitz A, Schaefer L, Tegeder I.
Progranulin Deficient Mice Develop Nephrogenic Diabetes Insipidus.
Aging Dis. 2018 Oct 1;9(5):817-830.
doi: 10.14336/AD.2017.1127.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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Rabbit anti Arginine Vasopressin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Arg-Vasopressin

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Product Code Applications Pack Size List Price Your Price Qty
AHP372
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C 0.1 ml loader
List Price Your Price
loader

Rabbit anti arginine vasopressin antibody recognizes arginine vasopressin. Binding is completely blocked with the arginine vasopression peptide. Cells are found in the paraventricular and supraoptic nucleus and fibers are found throughout the hypothalmus in rat brain of 4% FA perfused tissue.

Product Form
Serum - liquid
Antiserum Preparation
Antisera to Arginine Vasopressin were raised by repeated immunisation of rabbits with highly purified antigen. Whole serum is supplied - diluted.
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
CYFQNCPRG-Tg
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/500 1/5000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for Arg-Vasopressin antibody

  1. Suzuki, S. & Handa, R.J. (2005) Estrogen receptor-beta, but not estrogen receptor-alpha, is expressed in prolactin neurons of the female rat paraventricular and supraoptic nuclei: comparison with other neuropeptides.
    J Comp Neurol. 484: 28-42.
  2. Borrow, A.P. et al. (2018) Chronic Variable Stress Induces Sex-Specific Alterations in Social Behavior and Neuropeptide Expression in the Mouse.
    Endocrinology. 159 (7): 2803-2814.
  3. Hardt, S. et al. (2018) Progranulin Deficient Mice Develop Nephrogenic Diabetes Insipidus.
    Aging Dis. 9 (5): 817-30.

Immunofluorescence

RRID
AB_2062093
UniProt
P01186
Entrez Gene
Avp
GO Terms
GO:0001659 temperature homeostasis
GO:0002125 maternal aggressive behavior
GO:0003078 regulation of natriuresis
GO:0003084 positive regulation of systemic arterial blood pressure
GO:0005185 neurohypophyseal hormone activity
GO:0005615 extracellular space
GO:0030141 stored secretory granule
GO:0006939 smooth muscle contraction
GO:0007204 elevation of cytosolic calcium ion concentration
GO:0007621 negative regulation of female receptivity
GO:0007625 grooming behavior
GO:0007626 locomotory behavior
GO:0008284 positive regulation of cell proliferation
GO:0014049 positive regulation of glutamate secretion
GO:0030307 positive regulation of cell growth
GO:0030425 dendrite
GO:0031394 positive regulation of prostaglandin biosynthetic process
GO:0031894 V1A vasopressin receptor binding
GO:0031895 V1B vasopressin receptor binding
GO:0031896 V2 vasopressin receptor binding
GO:0032849 positive regulation of cellular pH reduction
GO:0035094 response to nicotine
GO:0035176 social behavior
GO:0042310 vasoconstriction
GO:0042538 hyperosmotic salinity response
GO:0042711 maternal behavior
GO:0043084 penile erection
GO:0045471 response to ethanol
GO:0045907 positive regulation of vasoconstriction
GO:0051970 negative regulation of transmission of nerve impulse

AHP372

158732 162807 180720

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