Rabbit anti Human adiponectin antibody recognizes an epitope within the C-terminal region (CT) of the 244 amino acid protein adiponectin (also known as Acrp30), a major adipokine secreted into the bloodstream from adipose tissue to modulate metabolism, including glucose regulation and fatty acid catabolism.
Unlike most other adipokines, Adiponectin is secreted exclusively by differentiating adipocytes at reduced levels during obesity. The serum level of Adiponectin is inversely correlated with BMI (body mass index) of an individual, and has an anti-inflammatory action, playing an important role in type II diabetes (insulin sensitivity) and atherosclerosis.
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to Human Adiponectin (CT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- A 15 amino acid peptide located near human Adiponectin carboxy-terminus.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
glucose metabolic process
generation of precursor metabolites and energy
fatty acid beta-oxidation
response to glucose stimulus
positive regulation of protein kinase A signaling cascade
negative regulation of macrophage derived foam cell differentiation
negative regulation of tumor necrosis factor-mediated signaling pathway
positive regulation of cholesterol efflux
positive regulation of glucose import
negative regulation of granulocyte differentiation
negative regulation of tumor necrosis factor production
positive regulation of myeloid cell apoptosis
negative regulation of heterotypic cell-cell adhesion
low-density lipoprotein particle clearance
response to tumor necrosis factor
protein homodimerization activity
negative regulation of I-kappaB kinase/NF-kappaB cascade
eukaryotic cell surface binding
negative regulation of fat cell differentiation
negative regulation of macrophage differentiation
negative regulation of low-density lipoprotein particle receptor biosynthetic process
negative regulation of gluconeogenesis
positive regulation of fatty acid metabolic process
negative regulation of inflammatory response
negative regulation of phagocytosis
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Adiponectin antibody
|Immunohistology - Paraffin 1
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Rat brain.
- Western Blotting
- AHP1005 detects a band of approximately 26.4kDa in human HL60 cell lysates
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Secondary Antibodies Available
Useful Reagents Available