Adiponectin antibody

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Rabbit anti Adiponectin (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1005 F P* WB 0.1 mg
Rabbit anti Human adiponectin antibody recognizes an epitope within the C-terminal region (CT) of the 244 amino acid protein adiponectin (also known as Acrp30), a major adipokine secreted into the bloodstream from adipose tissue to modulate metabolism, including glucose regulation and fatty acid catabolism.

Unlike most other adipokines, Adiponectin is secreted exclusively by differentiating adipocytes at reduced levels during obesity. The serum level of Adiponectin is inversely correlated with BMI (body mass index) of an individual, and has an anti-inflammatory action, playing an important role in type II diabetes (insulin sensitivity) and atherosclerosis.

Product Details

Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to Human Adiponectin (CT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide (NaN3)
Immunogen
A 15 amino acid peptide located near human Adiponectin carboxy-terminus.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q15848 Related reagents
Entrez Gene
ADIPOQ Related reagents
GO Terms
GO:0005125 cytokine activity
GO:0005179 hormone activity
GO:0005615 extracellular space
GO:0006006 glucose metabolic process
GO:0006091 generation of precursor metabolites and energy
GO:0006635 fatty acid beta-oxidation
GO:0009749 response to glucose stimulus
GO:0010739 positive regulation of protein kinase A signaling cascade
GO:0010745 negative regulation of macrophage derived foam cell differentiation
GO:0010804 negative regulation of tumor necrosis factor-mediated signaling pathway
GO:0010875 positive regulation of cholesterol efflux
GO:0046326 positive regulation of glucose import
GO:0030853 negative regulation of granulocyte differentiation
GO:0032720 negative regulation of tumor necrosis factor production
GO:0033034 positive regulation of myeloid cell apoptosis
GO:0034115 negative regulation of heterotypic cell-cell adhesion
GO:0034383 low-density lipoprotein particle clearance
GO:0034612 response to tumor necrosis factor
GO:0042593 glucose homeostasis
GO:0042803 protein homodimerization activity
GO:0043124 negative regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043499 eukaryotic cell surface binding
GO:0045599 negative regulation of fat cell differentiation
GO:0045650 negative regulation of macrophage differentiation
GO:0045715 negative regulation of low-density lipoprotein particle receptor biosynthetic process
GO:0045721 negative regulation of gluconeogenesis
GO:0045923 positive regulation of fatty acid metabolic process
GO:0050728 negative regulation of inflammatory response
GO:0050765 negative regulation of phagocytosis
Regulatory
For research purposes only

Applications of Adiponectin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Paraffin 1 10ug/ml
Western Blotting 1 2ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Rat brain.
Western Blotting
AHP1005 detects a band of approximately 26.4kDa in human HL60 cell lysates

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

Further Reading

  1. Wong, G.W. et al. (2004) A family of Acrp30/adiponectin structural and functional paralogs.
    Proc Natl Acad Sci U S A. 101 (28): 10302-7.
  2. Lihn, A.S. et al. (2005) Adiponectin: action, regulation and association to insulin sensitivity.
    Obes Rev. 6 (1): 13-21.
  3. Trayhurn, P. & Wood, I.S. (2005) Signalling role of adipose tissue: adipokines and inflammation in obesity.
    Biochem Soc Trans. 33 (Pt 5): 1078-81.