IgM antibody

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Goat anti Pig IgM

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: IgM

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References (11)
Applications
Storage Information
Batch/Lot Specific Datasheets

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AAI48 Datasheet SDS SDS	C E * WB	1 mg		Log in
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Anti Pig IgM Antibody gallery image 1 — **Published customer image:**
HRP conjugated Goat anti Pig IgM antibody (**AAI48P**) used to evaluate IgM levels in porcine serum samples by ELISA.
**Image caption:**
Experiment 1: Isotype profile of antibodies against *S. suis* serotype 7 in vaccinated sows and their piglets. Blood samples were collected 7 weeks and 1 week before farrowing from 25 vaccinated sows to analyze the immune response. The vaccination protocol is shown in Fig. Fig.1.1. IgM (a) titers were determined by ELISA. Randomly selected piglets (n = 125; 5 piglets/sow) from vaccinated sows were sampled at 7 days of age to evaluate titers of IgM (d) against *S. suis* serotype 7. Individual antibody titers are shown with horizontal bars representing mean ± SEM. Values significantly different are shown in the graphs with corresponding P value.

From: Corsaut L, Martelet L, Goyette-Desjardins G, Beauchamp G, Denicourt M, Gottschalk M, Segura M.
Immunogenicity study of a *Streptococcus suis* autogenous vaccine in preparturient sows and evaluation of passive maternal immunity in piglets.
**BMC Vet Res. 2021 Feb 5;17(1):72**.
doi: 10.1186/s12917-021-02774-4..
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Anti Pig IgM Antibody gallery image 2 — **Published customer image:**
Horseradish peroxidase conjugated Goat anti Pig IgM antibody used for the evaluation of IgM levels in healthy and disease porcine samples.
**Image caption:**
Total Ig (A), IgM (B), IgG1 (C) and IgG2 (D) levels1 in pigs either diseased²as suspected of *S. suis* (n = 20) with neurological signs, or in control pen-mates (n = 28) at different times post-weaning when cases were detected in the nursery phase.¹Points indicate means for n cases on a same day post-weaning and among the values for three isolates (isolate as random effect and pig as a repeated measure).²Defined as severe diseased pigs including central nervous system dysfunctions (opisthotonos, ataxia, and generalized tremor). The shadded areas indicates 95% confidence interval.et a.l

From: Fabà, L. Aragon, V.
Metabolic insights and background from naturally affected pigs during *Streptococcus suis* outbreaks.
**Transl Anim Sci. 7 (1) txad126**.
doi: 10.1093/tas/txad126.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Anti Pig IgM Antibody gallery image 3 — **Published customer image:**
Goat anti Pig IgM antibody (**AAI48**) used as a capture antibody for the assessment of IgM levels in porcine serum by ELISA
**Image caption:**
Effect of MLV and wild PRRSV strains on other infection parameters. Control piglets (CTRL) and piglets infected with wild VR2332, Lelystad and a mixture of MLV strains (VACC) were monitored throughout the experiment for their weight gain (calculated back to 0 dpi as 100%) after two weeks. Total IgM (G), IgA (H), and IgG (I) from sera were also analyzed by ELISA. Note that data are expressed as percent changes in individual animals (calculated back to 0 dpi as 100%). Time points at which infected piglets are significantly different from CTRL

From: Stepanova K, Toman M, Sinkorova J, Sinkora S, Pfeiferova S, Kupcova Skalnikova H, Abuhajiar S, Moutelikova R, Salat J, Stepanova H, Nechvatalova K, Leva L, Hermanova P, Kratochvilova M, Dusankova B, Sinkora M Jr, Horak V, Hudcovic T, Butler JE, Sinkora M.
Modified live vaccine strains of porcine reproductive and respiratory syndrome virus cause immune system dysregulation similar to wild strains.
**Front Immunol. 2024 Jan 12;14:1292381**.
doi: 10.3389/fimmu.2023.1292381.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Anti Pig IgM Antibody gallery image 4 — **Published customer image:**
Goat anti Pig IgM antibody (**AAI48P**) used for the evaluation of IgM levels following virus exposure.
**Image caption:**
Plot of dynamic ranges of IgM and IgG response against ASFV challenge. The animals were exposed to ASFV contaminated environment at 0 dpe. Samples were collected at seven designated time points: 0, 4, 8, 10, 12, 14, and 21 dpe. The onset of viremia in the source animals was previously confirmed by ASFV PCR to be between 8 and 10 dpe. The samples were tested for IgM and IgG, respectively. Average sample‐to‐positive ratio was calculated from 6 sets of samples at each time point. The vertical lines indicate the standard error. The results indicated that the initial detection of IgM occurred at 12 dpe. The initial detection of IgG occurred at 14 dpe, which was 2 days after the initial IgM detection.

From: Wu P, McDaniel AJ, Rodríguez YY, O'Donnell V, Jia W.
A Novel In-Cell ELISA With Superior Sensitivity and Specificity for the Detection of African Swine Fever Virus-Specific IgM and IgG Antibodies.
**Transbound Emerg Dis. 2026 Jan 5;2026:6272844**.
doi: 10.1155/tbed/6272844.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Anti Pig IgM Antibody gallery image 5 — **Published customer image:**
Horseradish peroxidase conjugated Goat anti Porcine IgM antibbody (**AAI48P**) used as a detection reagent for the measurement of antigen specific IgM in sera of pigs by ELISA
**Image caption:**
ELISA assessment of ASFV-pI215L immunogenicity after incubation of full-length recombinant ASFV-pI215L with sera collected from ASFV-infected pigs, showing late detection of IgG, early detection of IgM, and decrease of IgG recognition with analysis of multiple-residue mutations. (A) Absorbance detection of total IgM or total IgG after ASFV-pI215L^WT incubation with eight sera pools ranging from 0 to 35-72 dpi.

From: Jordão N, Urbano AC, Boinas F, Martins C, Ferreira F.
Multiple directed mutagenesis reduces enzymatic activity and antibody recognition of the African Swine Fever Virus E2 ubiquitin-conjugating protein (ASFV-pI215L).
**Emerg Microbes Infect. 2026 Dec;15(1):2622218**.
doi: 10.1080/22221751.2026.2622218.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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Goat anti Pig IgM antibody recognizes porcine IgM and shows no cross-reactivity with other porcine immunoglobulin classes in immunoelectrophoresis. This antibody may cross-react with IgM from other species.

Target Species: Pig
Product Form: Purified IgG - liquid
Antiserum Preparation: Antisera to porcine IgM were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography using antigen coupled to agarose beads.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Purified Porcine IgM
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

This product is shipped at ambient temperature.
Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA ¹	1/1000	1/30,000
Immunohistology - Frozen	1/200	1/2000
Western Blotting	1/1000	1/30,000

¹For coating plates a 1/100 - 1/500 dilution is recommended.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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Description	Rabbit anti Goat IgG (Fc):FITC
Rabbit anti Goat IgG (Fc):HRP	STAR122P	C E WB	1 mg	Log in
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Description	Rabbit anti Goat IgG (Fc):HRP

References for IgM antibody

Williams, A.R. et al. (2017) Dietary cinnamaldehyde enhances acquisition of specific antibodies following helminth infection in pigs.
Vet Immunol Immunopathol. 189: 43-52.
Corsaut, L. et al. (2020) Field Study on the Immunological Response and Protective Effect of a Licensed Autogenous Vaccine to Control Streptococcus suis Infections in Post-Weaned Piglets.
Vaccines (Basel). 8 (3)Jul 14 [Epub ahead of print].
Corsaut, L. et al. (2021) Immunogenicity study of a Streptococcus suis. autogenous vaccine in preparturient sows and evaluation of passive maternal immunity in piglets.
BMC Vet Res. 17 (1): 72.
Forner, R. et al. (2021) Distribution difference of colostrum-derived B and T cells subsets in gilts and sows.
PLoS One. 16 (5): e0249366.
López-Serrano, S. et al. (2023) Immune responses following neonatal vaccination with conserved F4 fragment of VtaA proteins from virulent Glaesserella parasuis adjuvanted with CAF®01 or CDA.
Vaccine X. 14: 100330.
Fabà, L. et al. (2023) Metabolic insights and background from naturally affected pigs during Streptococcus suis outbreaks
Translational Animal Science. txad126.
Urbano, A.C. et al. (2023) Targeted mutagenesis of the β-strand DNA binding region of African swine fever virus histone-like protein (pA104R) impairs DNA-binding activity and antibody recognition.
Antiviral Res. : 105784.
Maciag, S. et al. (2022) Effects of freezing storage on the stability of maternal cellular and humoral immune components in porcine colostrum.
Vet Immunol Immunopathol. 254: 110520.

View The Latest Product References

Stepanova, K. et al. (2023) Modified live vaccine strains of porcine reproductive and respiratory syndrome virus cause immune system dysregulation similar to wild strains.
Front Immunol. 14: 1292381.
Wu, P. et al. (2026) A Novel In-Cell ELISA With Superior Sensitivity and Specificity for the Detection of African Swine Fever Virus-Specific IgM and IgG Antibodies.
Transbound Emerg Dis. 2026: 6272844.
Jordão, N. et al. (2026) Multiple directed mutagenesis reduces enzymatic activity and antibody recognition of the African Swine Fever Virus E2 ubiquitin-conjugating protein (ASFV-pI215L).
Emerg Microbes Infect. 15 (1): 2622218.