IgG Antibody

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Horseradish peroxidase conjugated Goat anti Pig IgG (Fc) antibody used for the detection of pig seum antibodies in ELISA.
Image caption:
Kinetics of serum anti-cysticercal antibodies and HP10 secretion antigen levels after implantation of activated oncospheres in pigs. Two doses of activated oncospheres (500 or 1000) were surgically implanted in the subarachnoid space (SA) of the brain or in muscular tissues (IM). Two pigs were not infected (control). (A): Serum antibody levels. (B): Serum HP10 secretion antigen levels.

From: Fleury A, Trejo A, Cisneros H, García-Navarrete R, Villalobos N, Hernández M, et al. (2015) Taenia solium: Development of an Experimental Model of Porcine Neurocysticercosis.
PLoS Negl Trop Dis 9(8): e0003980.

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IgG Antibody gallery image 2

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Horseradish peroxidase conjugated Horseradish peroxidase conjugated Goat anti Porcine IgG(Fc) (AAI41P) antibody used for the evaluation of Va HRV specific antibodies in piglet sera by ELISA.
Image caption:
Wa HRV specific porcine Abs in piglet's sera during the experience. The isotype-specific and VN Abs to Wa HRV in serum samples were determined weekly by ELISA and VN assay. The arrow indicates the experimental inoculation with VirWa HRV (0 PID). PID: post inoculation days.

From: Vega CG, Bok M, Vlasova AN, Chattha KS, Fernández FM, Wigdorovitz A, et al. (2012) IgY Antibodies Protect against Human Rotavirus Induced Diarrhea in the Neonatal Gnotobiotic Piglet Disease Model. PLoS ONE 7(8): e42788.

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IgG Antibody gallery image 3

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Horseradish peroxidase conjugated Goat anti Porcine IgA antibody used for the evaluation of IgA levels in serum and nasal swabs following influenza infection by ELISA.
Image caption:
Antibodies in serum and nasal swabs. (A) Influenza total anti-NP antibodies in serum were analyzed by ELISA competition assay. (B) Influenza IgA anti-NP antibodies in nasal swabs were analyzed by ELISA at day 0, 21 and 28 PI. White squares, white rhomboids, white triangles and black circles represent average values from animals belonging to Mock/Mock (□), SwH1N1/Mock (◇), Mock/pH1N1 (△) and SwH1N1/pH1N1 (●) group, respectively. Error bars represent one SD above and below the mean.

From: Busquets N, Segalés J, Córdoba L, Mussá T, Crisci E, Martín-Valls GE, Simon-Grifé M, Pérez-Simó M, Pérez-Maíllo M, Núñez JI, Abad FX, Fraile L, Pina S, Majó N, Bensaid A, Domingo M, Montoya M.
Experimental infection with H1N1 European swine influenza virus protects pigs from an infection with the 2009 pandemic H1N1 human influenza virus.
Vet Res. 2010 Sep-Oct;41(5):74.

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IgG Antibody gallery image 4

Published customer image:
Horseradish peroxidase conjugated Goat anti Porcine IgA antibody used for the evaluation of IgA levels in serum and nasal swabs following influenza infection by ELISA.
Image caption:
IgA and IgG antibodies in serum. (A) Influenza IgA and (B) IgG anti-NP antibodies in serum were analyzed by ELISA at day 0, 21 and 28 PI. White squares, white rhomboids, white triangles and black circles represent mean values from animals belonging to Mock/Mock (□), SwH1N1/Mock (◇), Mock/pH1N1 (△) and SwH1N1/pH1N1 (●) groups, respectively. Error bars represent one SD above and below the mean.

From: Busquets N, Segalés J, Córdoba L, Mussá T, Crisci E, Martín-Valls GE, Simon-Grifé M, Pérez-Simó M, Pérez-Maíllo M, Núñez JI, Abad FX, Fraile L, Pina S, Majó N, Bensaid A, Domingo M, Montoya M.
Experimental infection with H1N1 European swine influenza virus protects pigs from an infection with the 2009 pandemic H1N1 human influenza virus.
Vet Res. 2010 Sep-Oct;41(5):74.

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  • IgG Antibody thumbnail image 1
  • IgG Antibody thumbnail image 2
  • IgG Antibody thumbnail image 3
  • IgG Antibody thumbnail image 4
  • Goat anti Pig IgG (Fc):HRP
  • Goat anti Pig IgG (Fc):FITC
  • Goat anti Pig IgG (Fc)
  • Goat anti Pig IgG (Fc):Biotin
  • Goat anti Pig IgG (Fc):Alk. Phos.
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
5 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AAI41ABEdatasheet pdfdatasheet pdf0.5 mg
    AAI41AB
    AAI41PEdatasheet pdfdatasheet pdf1 mg
    AAI41P
    AAI41FC, Fdatasheet pdfdatasheet pdf1 mg
    AAI41F
    AAI41E, ID, WBdatasheet pdfdatasheet pdf1 mg
    AAI41
    AAI41BE, WBdatasheet pdfdatasheet pdf1 mg
    AAI41B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Goat anti Pig IgG (Fc) antibody recognizes the Fc region of the porcine IgG heavy chains and shows no cross - reactivity with other porcine immunoglobulin classes as evaluated by immunoelectrophoresis. Goat anti Pig IgG (Fc) has not been species cross adsorbed and may react with the Fc region of IgG from other species.

      Goat anti Pig IgG (Fc) antibody has been used extensively as a detection reagent for porcine IgG in ELISA, for example monitoring of the IgG response in influenza infected pigs (Crisci et al. 2013).
    • Intended Use
    • Target Species
      Pig
    • Product Form
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG fraction conjugated to Biotin - liquid
      Purified IgG conjugated to Alkaline Phosphatase - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to porcine IgG were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      0.1% Proclin™ 300
      0.2% Bovine Serum Albumin
      0.09%Sodium Azide
      0.2%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09% Sodium Azide (NaN3)
      0.2% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      0.2% Bovine Serum Albumin
    • Immunogen
      Purified porcine IgG.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      50mM HEPES, 0.1M NaCl, 1mM MgCl2, 0.1mM ZnCl2
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted.
      Store at +4oC.
      DO NOT FREEZE.

      This product should be stored undiluted.
      Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
      12 months from date of despatch.
      18 months from date of despatch.
      12 months from date of despatch.
      12 months from date of despatch.
    • Acknowledgements
      Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10,0001/100,000
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/201/100
      Immunohistology - Frozen1/201/200
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1001/1000
      Immunodiffusion
      Western Blotting1/10001/30,000
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1:100001:100000
      Western Blotting1:100001:100000
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/50001/50000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IgG Antibody Formats

    Formats Applications Sizes available
    IgG Antibody : Alk. Phos. E 0.5 mg
    IgG Antibody : FITC C, F 1 mg
    IgG Antibody : HRP E 1 mg
    IgG Antibody : Biotin E, WB 1 mg
    IgG Antibody : Purified E, ID, WB 1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B

          Recommended Positive Controls

            Histology Controls

              References

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