IgA Antibody

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IgA Antibody gallery image 1

Published customer image:
Horseradish peroxidase conjugated Goat anti porcine IgA antibody (AAI40P) used for the evaluation of C. trachomitis specific serum IgA antibodies in piglets by ELISA.
Image caption:
Serum antibodies. Detection of serum antibodies against C. trachomatis, measured as the difference in OD values day (x) and day 0.

From: Erneholm K, Lorenzen E, Bøje S, Olsen AW, Andersen P, Cassidy JP, Follmann F, Jensen HE, Agerholm JS.
Genital tract lesions in sexually mature Göttingen minipigs during the initial stages of experimental vaginal infection with Chlamydia trachomatis serovar D.
BMC Vet Res. 2016 Sep 10;12(1):200..

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IgA Antibody gallery image 2

Published customer image:
Horseradish peroxidase conjugated Horseradish peroxidase conjugated Goat anti Porcine IgA antibody (AAI40P) used for the evaluation of Va HRV specific antibodies in piglet sera by ELISA.
Image caption:
Wa HRV specific porcine Abs in piglet's sera during the experience. The isotype-specific and VN Abs to Wa HRV in serum samples were determined weekly by ELISA and VN assay. The arrow indicates the experimental inoculation with VirWa HRV (0 PID). PID: post inoculation days.

From: Vega CG, Bok M, Vlasova AN, Chattha KS, Fernández FM, Wigdorovitz A, et al. (2012) IgY Antibodies Protect against Human Rotavirus Induced Diarrhea in the Neonatal Gnotobiotic Piglet Disease Model. PLoS ONE 7(8): e42788.

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IgA Antibody gallery image 3

Published customer image:
Horseradish peroxidase conjugated Goat anti Porcine IgA antibody used for the evaluation of IgA levels in serum and nasal swabs following influenza infection by ELISA.
Image caption:
Antibodies in serum and nasal swabs. (A) Influenza total anti-NP antibodies in serum were analyzed by ELISA competition assay. (B) Influenza IgA anti-NP antibodies in nasal swabs were analyzed by ELISA at day 0, 21 and 28 PI. White squares, white rhomboids, white triangles and black circles represent average values from animals belonging to Mock/Mock (□), SwH1N1/Mock (◇), Mock/pH1N1 (△) and SwH1N1/pH1N1 (●) group, respectively. Error bars represent one SD above and below the mean.

From: Busquets N, Segalés J, Córdoba L, Mussá T, Crisci E, Martín-Valls GE, Simon-Grifé M, Pérez-Simó M, Pérez-Maíllo M, Núñez JI, Abad FX, Fraile L, Pina S, Majó N, Bensaid A, Domingo M, Montoya M.
Experimental infection with H1N1 European swine influenza virus protects pigs from an infection with the 2009 pandemic H1N1 human influenza virus.
Vet Res. 2010 Sep-Oct;41(5):74.

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IgA Antibody gallery image 4

Published customer image:
Horseradish peroxidase conjugated Goat anti Porcine IgA antibody used for the evaluation of IgA levels in serum and nasal swabs following influenza infection by ELISA.
Image caption:
IgA and IgG antibodies in serum. (A) Influenza IgA and (B) IgG anti-NP antibodies in serum were analyzed by ELISA at day 0, 21 and 28 PI. White squares, white rhomboids, white triangles and black circles represent mean values from animals belonging to Mock/Mock (□), SwH1N1/Mock (◇), Mock/pH1N1 (△) and SwH1N1/pH1N1 (●) groups, respectively. Error bars represent one SD above and below the mean.

From: Busquets N, Segalés J, Córdoba L, Mussá T, Crisci E, Martín-Valls GE, Simon-Grifé M, Pérez-Simó M, Pérez-Maíllo M, Núñez JI, Abad FX, Fraile L, Pina S, Majó N, Bensaid A, Domingo M, Montoya M.
Experimental infection with H1N1 European swine influenza virus protects pigs from an infection with the 2009 pandemic H1N1 human influenza virus.
Vet Res. 2010 Sep-Oct;41(5):74.

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  • IgA Antibody thumbnail image 1
  • IgA Antibody thumbnail image 2
  • IgA Antibody thumbnail image 3
  • IgA Antibody thumbnail image 4
  • Goat anti Pig IgA:Biotin
  • Goat anti Pig IgA:FITC
  • Goat anti Pig IgA:Alk. Phos.
  • Goat anti Pig IgA
  • Goat anti Pig IgA:HRP
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
5 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AAI40ABC, E, WBdatasheet pdfdatasheet pdf0.5 mg
    AAI40AB
    AAI40BE, WBdatasheet pdfdatasheet pdf1 mg
    AAI40B
    AAI40FC, Fdatasheet pdfdatasheet pdf1 mg
    AAI40F
    AAI40E, IDdatasheet pdfdatasheet pdf1 mg
    AAI40
    AAI40PEdatasheet pdfdatasheet pdf1 mg
    AAI40P
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Goat anti pig IgA antibody recognizes porcine IgA and shows no cross-reactivity with other porcine immunoglobulin classes as assessed by immunoelectrophoresis. This antibody may cross-react with IgA from other species.

      Goat anti Porcine IgA antibody has been succesfully used for the evaluation of porcine IgA levels in body fluids of pigs by both ELISA and Western blotting.
    • Intended Use
    • Target Species
      Pig
    • Product Form
      Purified IgG conjugated to Biotin - liquid
      Purified IgG fraction conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Alkaline Phosphatase - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to porcine IgA were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.2% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      0.2% Bovine Serum Albumin
      0.09%Sodium Azide
      0.2%Bovine Serum Albumin
      0.09%Sodium Azide
      0.1% Proclin™ 300
      0.2% Bovine Serum Albumin
    • Immunogen
      Purified porcine IgA.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.5mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      50mM HEPES
      0.1M NaCl
      1mM MgCl2
      0.1mM ZnCl2
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.
      DO NOT FREEZE.

      This product should be stored undiluted.
      Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
      12 months from date of despatch.
      12 months from date of despatch.
      18 months from date of despatch.
      12 months from date of despatch.
    • Acknowledgements
      Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1:100001:100000
      Western Blotting1:100001:100000
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunohistology - Frozen1/501/500
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10001/10000
      Immunohistology - Frozen1/1001/1000
      Western Blotting1/10001/10000
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1001/30000
      Immunodiffusion
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10,0001/100,000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IgA Antibody Formats

    Formats Applications Sizes available
    IgA Antibody : Biotin E, WB 1 mg
    IgA Antibody : HRP E 1 mg
    IgA Antibody : Purified E, ID 1 mg
    IgA Antibody : Alk. Phos. C, E, WB 0.5 mg
    IgA Antibody : FITC C, F 1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B

          Recommended Positive Controls

            Histology Controls

              References

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