MHC Class II H-2I-Ak/s/R Antibody | ER-TR2

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  • Rat anti Mouse MHC Class II H-2I-Ak/s/R
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    ER-TR2
  • Isotype
    IgG2b
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2400C, Fdatasheet pdfdatasheet pdf0.25 mg
    MCA2400
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rat anti Mouse MHC Class II H-2I-Ak/s/R antibody, clone ER-TR2 recognizes a polymorphic determinant of the mouse H-2I-A MHC class II antigen, reacting with haplotypes k, s and r. Mouse strains with the haplotypes b, d, q and f are not recognized by this antibody.

      Mouse MHC class II antigens are expressed by dendritic cells, B-cells and macrophages. In thymus tissues, clone ER-TR2 stains cortical and medullary stromal cells.

      The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the H-2 region.

    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Isolated C3H thymic stromal cells.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Cells from immunised rats were fused with cells of the mouse P3-X63-Ag8.56 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/1001/200
      Immunohistology - Frozen

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional MHC Class II H-2I-Ak/s/R Antibody Formats

    Formats Clone Applications Sizes available
    MHC Class II H-2I-Ak/s/R Antibody : Purified ER-TR2 C, F 0.25 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Source Reference

              References

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