IL-12 Antibody

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  • Goat anti Mouse Interleukin-12
  • Goat anti Mouse Interleukin-12:Biotin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AAM33E, FN, WBdatasheet pdfdatasheet pdf0.1 mg
    AAM33
    AAM33BE, WBdatasheet pdfdatasheet pdf50 µg
    AAM33B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Goat anti Mouse Interleukin-12 antibody recognizes mouse interleukin-12 (IL-12).

      Interleukin-12 is produced by activated B lymphocytes and macrophages. The properties of IL-12 include the stimulation of growth of activated T lymphocytes and the development of TH1 CD4 +ve cells and CD8 +ve cytotoxic T cells.

      Goat anti Mouse Interleukin-12 antibody neutralizes murine IL-12 activity with an ND50 for mIL-12 (2ngml-1 of between 0.002 and 0.002μg ml-1.
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG - lyophilised
      Purified IgG conjugated to Biotin - lyophilised
    • Reconstitution
      Reconstitute with 0.1ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
      N.B. For functional studies do not add sodium azide
      Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albumin
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
    • Antiserum Preparation
      Antisera to mouse IL-12 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      None present
      None present
    • Immunogen
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1mg/ml after reconstitution.
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      12 months from date of reconstitution.
    • GO Terms
      negative regulation of growth of symbiont in host
      growth factor activity
      immune response
      interleukin-12 complex
      positive regulation of activated T cell proliferation
      positive regulation of interferon-gamma production
      protein homodimerization activity
      T-helper cell differentiation
      cytokine activity
      membrane
      interleukin-12 receptor binding
      positive regulation of T-helper 1 type immune response
      positive regulation of T cell proliferation
      negative regulation of inflammatory response to antigenic stimulus
      cytokine receptor activity
      positive regulation of defense response to virus by host
      positive regulation of T cell mediated cytotoxicity
      interleukin-12 beta subunit binding
      defense response to protozoan
      interleukin-12 alpha subunit binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.52.0 ug/ml
      Functional Assays2.03.0 ng/ml
      Western Blotting0.10.2 ug/ml
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.251.0ug/ml
      Western Blotting0.10.2ug/ml

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This purified mouse IL-12 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with a biotinylated mouse IL-12 antibody (AAM33B) as the detection reagent and recombinant mouse IL-12 (PMP40) as the standard.
    • ELISA
      This biotinylated mouse IL-12 antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with a purified mouse IL-12 antibody (AAM33) as the capture reagent and recombinant mouse IL-12 (PMP40) as the standard.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      This purified mouse IL-12 antibody may used in Western Blotting applications under either reducing or non-reducing conditions with recombinant mouse IL-12 (PMP40) as the positive control.
    • Western Blotting
      This biotinylated mouse IL-12 antibody may used in Western Blotting application under either reducing of non-reducing conditions with recombinant mouse IL-12 (PMP40) as the positive control.
    • Instructions For Use
    • Instructions For Use

    Additional IL-12 Antibody Formats

    Formats Applications Sizes available
    IL-12 Antibody : Biotin E, WB 50 µg
    IL-12 Antibody : Purified E, FN, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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