Rat anti Mouse IgM heavy chain recognizes murine IgM (mu heavy chain) and does not cross react with other murine immunoglobulin classes or subclasses.
Avidity = 2 x 108 M-1
Purified IgM - liquid
Purified IgM prepared by affinity chromatography on mouse IgM/Sepharose 4B from tissue culture supernatant
Polyclonal mouse IgM.
Approx. Protein Concentrations
Isotype concentration 1 mg/ml
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Spleen cells from immunized Lou/c rats were fused with cells of the rat IR983F myeloma cell line.
Store at +4oC or at -20oC if preferred.This product should be stored undiluted.Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
This antibody may be used as a coating antibody in sandwich ELISA in combination with LO-MM-9 (MCA199B) and Streptavidin:HRP (STAR5B) as the detection reagents.