Detection of Rabbit anti Mouse TNFα:biotin (AAM19B) in a sandwich ELISA with recombinant murine TNFα (PMP24) as the antigen and Rat anti Mouse TNFα (MCA1487) as the coating antibody using Streptavidin:HRP (STAR5B)
Store at +4oC. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
18 months from date of despatch.
For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Immunohistology - Frozen
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate negative/positive controls.
1. Stager, S. et al. (2000) Immunization with a recombinant stage-regulated surface protein from Leishmania donovani induces protection against visceral Leishmaniasis. J. Immunol. 165: 7064-7071.
2. Weiss, D. et al. (2008) Bovine monocyte TLR2 receptors differentially regulate the intracellular fate of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium.J. Leukoc. Biol. 83: 48-55.
3. Wong, Y.L. et al. (2016) Adverse effects of hydroxyethyl starch (HES 130/0.4) on intestinal barrier integrity and metabolic function are abrogated by supplementation with Albumin.J Transl Med. 14: 60.