Streptavidin

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Detection of Rabbit anti Mouse TNFα:biotin (AAM19B) in a sandwich ELISA with recombinant murine TNFα (PMP24) as the antigen and Rat anti Mouse TNFα (MCA1487) as the coating antibody using Streptavidin:HRP (STAR5B)

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Detection of Mouse anti histidine tag:biotin (MCA1396B) in a Western blot analysis of a HIS tagged protein using Streptavidin:HRP (STAR5B)

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  • Streptavidin:HRP
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Accessory Reagent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    STAR5BC, E, P, WBdatasheet pdfdatasheet pdf1 mg
    STAR5B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Streptavidin: horseradish peroxidase conjugates show negligible non-specific binding to non-biotinylated macromolecules and therefore give very low backgrounds.
    • Intended Use
    • Product Form
      Purified Streptavidin conjugated to Horseradish Peroxidase (HRP) - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      HRP stabiliser (BUF052A)
      0.01% Thiomersal
    • Purity
    • Approx. Protein Concentrations
      Protein concentration 1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10001/10000
      Immunohistology - Frozen1/500
      Immunohistology - Paraffin1/500
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Streptavidin Formats

    Formats Applications Sizes available
    Streptavidin : HRP C, E, P, WB 1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A

          Recommended Positive Controls

            Histology Controls

              References

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