RPE conjugated Goat anti Mouse IfF antibody recognizes both the heavy and light chains of mouse IgG. The reagent has been adsorbed to minimise cross-reactivity with rat immunoglobulins and is therefore of particular value in detecting mouse primary antibodies bound to rat tissues.
We recommend diluting this product in buffer containing 10% normal rat serum to remove any remaining cross-reactivity.
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitute with 1.0ml distilled water
Antisera to mouse IgG were raised by repeated immunisation of goats with highly purified antigen. Purified IgG prepared by affinity chromatography.
Bovine Serum Albumin
Mouse IgG, whole molecule.
Approx. Protein Concentrations
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
This product should be stored undiluted.
DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Use 50ul of the suggested working dilution to label 106 cells in 100ul.