IgG Antibody

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IgG Antibody gallery image 1

Detection of Mouse anti Rabbit CD44 (MCA806GA) in a flow cytometric analysis of rabbit peripheral blood lymphocytes using FITC conjugated Goat anti Rabbit IgG H+L (STAR117F)

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IgG Antibody gallery image 2

Detection of Rabbit anti Human α-adaptin (AHP2216) in a flow cytometric analysis of mouse bone marrow cells using FITC conjugated Goat anti Mouse IgG H+L (STAR117F)

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IgG Antibody gallery image 3

Detection of Mouse anti Bovine CD28 (MCA5779) in a flow cytometric analysis of bovine peripheral blood monocytes using FITC conjugated Goat anti Mouse IgG H+L (STAR117F)

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IgG Antibody gallery image 4

Detection of Mouse anti Equine CD44 (MCA1082GA) in a flow cytometric analysis of equine peripheral blood lymphocytes using FITC conjugated Goat anti Mouse H+L (STAR117F)

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IgG Antibody gallery image 5

Detection of Mouse anti Ovine CD45 (MCA2220GA) in a flow cytometric analysis of ovine peripheral blood granulocytes using FITC conjugated Goat anti Mouse IgG H+L (STAR117F)

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IgG Antibody gallery image 6

Detection of Mouse anti Porcine CD107a (MCA2315GA) in a flow cytometric analysis of porcine peripheral blood granulocytes using Goat anti Mouse IgG H+L:FITC (STAR117F) following membrane permeablisation using Leucoperm(BUF09)

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IgG Antibody gallery image 7

Detection of Mouse anti Bovine MHC Class II DQ (MCA5655) in a flow cytometric analysis of bovine peripheral blood lymphocytes using FITC conjugated Goat anti Mouse IgG H+L (STAR117F)

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IgG Antibody gallery image 8

Detection of Mouse anti Guinea pig CD1b3 (MCA566GA) in a flow cytometric analysis of guinea pig splenocytes using FITC conjugated Goat anti Mouse IgG H+L (STAR117F)

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IgG Antibody gallery image 9

Detection of Mouse anti Equine MHC Class II (MCA1085GA) in a flow cytometric analysis of equine peripheral blood lymphocytes using FITC conjugated Goat anti Mouse IgG H+L (STAR117F)

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IgG Antibody gallery image 10

HeLa cells were fixed with 3% PFA and permeabilized with 0.3% Triton X-100. Nuclei were stained with 1.5 µg/ml DAPI. Immunofluorescence was carried out using MCA3518Z, a Mouse monoclonal antibody specific for the human tubulin alpha 4A chain at 1/50. STAR117D488GA, Goat anti Mouse IgG DyLight®488 was then applied at 1/50. Image was visualized on the Bio-Rad ZOE Fluorescent Cell Imager at 60x magnification

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IgG Antibody gallery image 11

Detection of Mouse anti Equine MHC Class I (monomorphic) (MCA1086GA) in a flow cytometric analysis of equine peripheral blood lymphocytes using FITC conjugated Goat anti Mouse IgG H+L (STAR117F)

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IgG Antibody gallery image 12

HeLa cells were fixed with 3% PFA and permeabilized with 0.3% Triton X-100. Nuclei were stained with 1.5 µg/ml DAPI. Immunofluorescence was carried out using MCA4845Z, a Mouse monoclonal antibody specific for human tubulin beta 2C at 1/50. STAR117D488GA, Goat anti Mouse IgG DyLight®488 was then applied at 1/50. Image was visualized on the Bio-Rad ZOE Fluorescent Cell Imager at 60x magnification

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IgG Antibody gallery image 13

Multiplex staining of HeLa whole cell lysate with Mouse anti PCNA (MCA1558) detected with Goat anti Mouse IgG (H/L):DyLight®680 (STAR117D680GA) shown in red and Rabbit anti GAPDH (AHP1628) detected with Sheep anti Rabbit IgG:DyLight®488 (STAR36D488GA) shown in green

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IgG Antibody gallery image 14

Multiplex staining of HeLa whole cell lysate with Mouse anti PCNA (MCA1558) detected with Goat anti Mouse IgG (H/L):DyLight®649 (STAR117D649GA) shown in red and Rabbit anti GAPDH (AHP1628) detected with Sheep anti Rabbit IgG:DyLight®488 (STAR36D488GA) shown in green

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IgG Antibody gallery image 15

Western blot analysis of NFkB IP samples. IP was performed on Jurkat cell lysates using 10 µg (lanes 2 & 7), 5 µg (lanes 3 & 8), 1 µg (lanes 4 & 9) Sheep anti NFkB p65 antibody (AHP394), and 10 µg polyclonal IgG (lanes 5 & 10).

8.75 µl of each IP was loaded onto an AnykD ™ Criterion ™ TGX Stain-Free™ gel. Precision Plus Protein™ Prestained Standards were run lanes 1 & 6.

Mouse anti NFkB p65 (MCA6021) was used at 1/1000 in lanes 1-5. HRP conjugated Goat anti Mouse IgG (H/L) antibody (STAR117P) was used at 1/10,000 and visualized on the Bio-Rad Chemidoc™ Touch Imaging System. Arrow points to NFkB (molecular weight 65 kDa).

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IgG Antibody gallery image 16

Paraffin section of human colon adenocarcinoma (HIER citrate pH6 antigen retrieval) blocked with 10% FCS and stained with mouse anti human cytokeratin 18 (MCA1864H) and detected with goat anti mouse IgG:A488 (STAR117D488GA). The tissue was also counterstained with PureBlu Hoechst 33342 (135-1304)

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  • Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
  • Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)
  • Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
  • Goat anti Mouse IgG (H/L):DyLight®405 (Multi Species Adsorbed)
  • Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
  • Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)
  • Goat anti Mouse IgG (H/L) (Multi Species Adsorbed)
  • Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
  • Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
  • Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
10 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    STAR117D549GAF, IF, WBdatasheet pdfdatasheet pdf0.1 mg
    STAR117D549GA
    STAR117D800GAF, IF, WBdatasheet pdfdatasheet pdf0.1 mg
    STAR117D800GA
    STAR117D405GAF, IFdatasheet pdfdatasheet pdf0.1 mg
    STAR117D405GA
    STAR117D680GAF, WBdatasheet pdfdatasheet pdf0.1 mg
    STAR117D680GA
    STAR117D649GAF, IFdatasheet pdfdatasheet pdf0.1 mg
    STAR117D649GA
    STAR117D488GAF, IFdatasheet pdfdatasheet pdf0.1 mg
    STAR117D488GA
    STAR117PE, WBdatasheet pdfdatasheet pdf0.5 mg
    STAR117P
    STAR117E, F, WBdatasheet pdfdatasheet pdf0.5 mg
    STAR117
    STAR117FFdatasheet pdfdatasheet pdf0.5 mg
    STAR117F
    STAR117AE, WBdatasheet pdfdatasheet pdf0.5 mg
    STAR117A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Goat anti Mouse IgG antibody recognizes mouse IgG and light chains common to other mouse immunoglobulin classes.

      Goat anti Mouse IgG has been cross-adsorbed using human, bovine, porcine, equine, lapine and chicken immunoabsorbants to remove cross-reactive antibodies. Less than 0.1% cross reactivity was detected to human, bovine, porcine, equine, caprine, lapine and chicken IgG by immunoelectrophoresis and ELISA.

      Goat anti Mouse IgG antibody is highly recommended for use as a secondary antibody with human and veterinary samples. Goat anti Mouse IgG antibody has been used successfully as a secondary detection reagent in combination with mouse clone CC327 for the detection of TNFα and mouse clone 8M6 for the detection of interleukin-8 in bovine respiritory syncitial virus infected, neonatal ovine lung tissue by immunohistochemistry (Redondo et al. 2013).
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
      Purified IgG conjugated to DyLight®549 - liquid
      Purified IgG conjugated to DyLight®800 - liquid
      Purified IgG conjugated to DyLight®405 - liquid
      Purified IgG conjugated to DyLight®680 - liquid
      Purified IgG conjugated to DyLight®649 - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Alkaline Phosphatase - liquid
      Purified IgG conjugated to DyLight®488 - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography
      Purified IgG prepared by affinity chromatography
      Purified IgG prepared by affinity chromatography
      Purified IgG prepared by affinity chromatography
      Purified IgG prepared by affinity chromatography
      Purified IgG prepared by affinity chromatography
      Purified IgG prepared by affinity chromatography
      Purified IgG prepared by affinity chromatography
    • Antiserum Preparation
      Antisera to mouse IgG were raised by repeated immunisations of goats with highly purified antigen.
    • Preservative Stabilisers
      0.1% Proclin™ 300
      0.2% Bovine Serum Albumin
      0.09%Sodium Azide (NaN3)
      0.09%Sodium Azide (NaN3)
      0.09%Sodium Azide (NaN3)
      0.09%Sodium Azide (NaN3)
      0.09%Sodium Azide (NaN3)
      0.1% Sodium Azide (NaN3)
      0.09%Sodium Azide
      0.2%Bovine Serum Albumin
      0.09%Sodium Azide
      0.2%Bovine Serum Albumin
      0.09%Sodium Azide (NaN3)
    • Immunogen
      Whole mouse IgG
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0mg/ml
      IgG concentration 0.5 mg/ml
      IgG concentration 0.5 mg/ml
      IgG concentration 0.5 mg/ml
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      50 mM HEPES pH 7.1
      0.1M NaCl
      1mM MgCl2
      0.1mM ZnCl2
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE

      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      phagocytosis, engulfment
      immunoglobulin complex, circulating
      extracellular region
      B cell receptor complex
      complement activation, classical pathway
      positive regulation of type IIa hypersensitivity
      antigen binding
      integral to membrane
      phagocytosis, recognition
      antibacterial humoral response
      positive regulation of type I hypersensitivity
      humoral immune response mediated by circulating immunoglobulin
      external side of plasma membrane
      antibody-dependent cellular cytotoxicity
      positive regulation of phagocytosis
      plasma membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
      DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
      DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
      DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
      DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
      DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
      DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10,0001/100,000
      Western Blotting1/10001/10,000
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/400
      Immunofluorescence1/501/400
      Western Blotting1/5001/1000
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunofluorescence
      Western Blotting1/100001/50000
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/250
      Immunofluorescence
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/5001/1000
      Western Blotting1/5001/1000
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/5001/1000
      Immunofluorescence1/5001/1000
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1ug/ml10ug/ml
      Flow Cytometry
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/200
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/50001/50000
      Western Blotting1/25001/25000
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/4001/800
      Immunofluorescence

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 50 ul of the suggested working dilution to label 1x106 cells in 100ul
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
      Bio-Rad recommends STAR88P as the detection reagent for STAR117 in western blotting applications.
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IgG Antibody Formats

    Formats Applications Sizes available
    IgG Antibody : DyLight®680 F, WB 0.1 mg
    IgG Antibody : DyLight®800 F, IF, WB 0.1 mg
    IgG Antibody : DyLight®405 F, IF 0.1 mg
    IgG Antibody : DyLight®649 F, IF 0.1 mg
    IgG Antibody : FITC F 0.5 mg
    IgG Antibody : HRP E, WB 0.5 mg
    IgG Antibody : DyLight®549 F, IF, WB 0.1 mg
    IgG Antibody : Purified E, F, WB 0.5 mg
    IgG Antibody : DyLight®488 F, IF 0.1 mg
    IgG Antibody : Alk. Phos. E, WB 0.5 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
      STAR88P

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B

          Recommended Positive Controls

            Histology Controls

              References

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