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IgG antibody

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Anti Rat IgG (H/L) Antibody thumbnail image 1
Anti Rat IgG (H/L) Antibody gallery image 1
Staining of mouse spleen cryosection with Rat anti Mouse CD68 antibody, clone FA-11 (MCA1957) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (305005) showing staining of macrophages in the red pulp.

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Goat anti Rat IgG (H/L):HRP

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG
Region
(H/L)

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305005
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Goat anti Rat IgG antibody reacts with the heavy and light chains of rat IgG, and the light chains of rat IgA and IgM. Cross reactivity with mouse immunoglobulins has been minimised by cross adsorption with pooled mouse sera and plasmacytoma/hybridoma proteins.

Target Species
Rat
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid.
Antiserum Preparation
Antiserum to rat IgG was raised by repeated immunisation of goats with highly purified antigen.
Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
50% Glycerol
Immunogen
Rat IgG
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/4000 1/8000
Immunoblotting
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for IgG antibody

  1. Liu, F. et al. (2015) Rapid and reliable healing of critical size bone defects with genetically modified sheep muscle.
    Eur Cell Mater. 30: 118-31.

305005

156908 157216 158930 161112

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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