CD31 Antibody | 2H8

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  • Hamster anti Mouse CD31:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    2H8
  • Isotype
    IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1370ZF, FN, IFdatasheet pdfdatasheet pdf0.5 mg
    MCA1370Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Hamster anti Mouse CD31 monoclonal antibody, clone 2H8 recognizes murine CD31, also known as Platelet endothelial cell adhesion molecule or PECAM-1. CD31 is a ~130kDa single pass type-1 membrane glycoprotein bearing six C2 Ig-like domains, expressed on all continuous endothelium including arteries, veins and non-sinusoidal capillaries. CD31 is also expressed on all haemopoietic lineages with the exception of the erythroid line (Bogen et al. 1992) .

      Hamster anti mouse CD31, clone 2H8 effectively inhibits transmigration of activated polymorphonuclear cells and monocytes across the endothelium. In a mouse model for acute peritonitis clone 2H8 blocks acute inflammation (Bogen et al. 1994). CD31 has also been shown to be critically involved in the sensing of changes in shear stress associated with atherosclerotic lesions and in the associated atherogenesis (Stevens et al. 2008).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry0.1ug/ml
      Functional Assays
      Immunofluorescence

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 5ul of the suggested working dilution to label 100ul of whole blood.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD31 Antibody Formats

    Formats Clone Applications Sizes available
    CD31 Antibody : Preservative Free 2H8 F, FN, IF 0.5 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Hamster (Armenian) IgG Negative ControlMCA23560.25 mgF
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        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Functional Assays
                Immunofluorescence

              References

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