CD204 Antibody | 2F8

//www.bio-rad-antibodies.com/mouse-cd204-antibody-2f8-mca1322.html?RenderPageSpecific=1
CD204 Antibody | 2F8 gallery image 1

Staining of mouse peritoneal macrophages with Rat anti Mouse CD204:FITC (MCA1322FB)

Enlarge
CD204 Antibody | 2F8 gallery image 2

Staining of J774 cell line with Rat anti Mouse CD204 (MCA1322)

Enlarge
CD204 Antibody | 2F8 gallery image 3

Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD204:FITC (MCA1322FA)

Enlarge
CD204 Antibody | 2F8 gallery image 4

Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Alexa Fluor® 488 (MCA1322A488)

Enlarge
CD204 Antibody | 2F8 gallery image 5

Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Alexa Fluor® 647 (MCA1322A647)

Enlarge
CD204 Antibody | 2F8 gallery image 6

Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Biotin (MCA1322B)

Enlarge
CD204 Antibody | 2F8 gallery image 7

Western Blot staining of J774 lysate (non reduced) probed with Rat anti Mouse CD204 (MCA1322)

Enlarge
CD204 Antibody | 2F8 gallery image 8

Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Low Endotoxin (MCA1322EL)

Enlarge
CD204 Antibody | 2F8 gallery image 9

Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD204 antibody, clone 2F8 (MCA1322) followed by horseradish peroxidase Goat anti Rat IgG antibody (STAR72). Low power

Enlarge
CD204 Antibody | 2F8 gallery image 10

Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD204 antibody, clone 2F8 (MCA1322) followed by horseradish peroxidase Goat anti Rat IgG antibody (STAR72). Medium power

Enlarge
CD204 Antibody | 2F8 gallery image 11

Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD204 antibody, clone 2F8 (MCA1322) followed by horseradish peroxidase Goat anti Rat IgG antibody (STAR72). High power

Enlarge
  • CD204 Antibody | 2F8 thumbnail image 1
  • CD204 Antibody | 2F8 thumbnail image 2
  • CD204 Antibody | 2F8 thumbnail image 3
  • CD204 Antibody | 2F8 thumbnail image 4
  • CD204 Antibody | 2F8 thumbnail image 5
  • CD204 Antibody | 2F8 thumbnail image 6
  • CD204 Antibody | 2F8 thumbnail image 7
  • CD204 Antibody | 2F8 thumbnail image 8
  • CD204 Antibody | 2F8 thumbnail image 9
  • CD204 Antibody | 2F8 thumbnail image 10
  • CD204 Antibody | 2F8 thumbnail image 11
  • Rat anti Mouse CD204:RPE
  • Rat anti Mouse CD204
  • Rat anti Mouse CD204:Biotin
  • Rat anti Mouse CD204:FITC
  • Rat anti Mouse CD204:Biotin
  • Rat anti Mouse CD204:FITC
  • Rat anti Mouse CD204:Alexa Fluor® 488
  • Rat anti Mouse CD204:RPE
  • Rat anti Mouse CD204:Alexa Fluor® 647
  • Rat anti Mouse CD204:Alexa Fluor® 488
  • Rat anti Mouse CD204
  • Rat anti Mouse CD204:FITC
  • Rat anti Mouse CD204
  • Rat anti Mouse CD204:FITC
  • Rat anti Mouse CD204:Low Endotoxin
  • Rat anti Mouse CD204:Alexa Fluor® 647
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    2F8
  • Isotype
    IgG2b
7 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1322FFdatasheet pdfdatasheet pdf0.1 mg
    MCA1322F
    MCA1322BFdatasheet pdfdatasheet pdf0.1 mg
    MCA1322B
    MCA1322GAC *, E, F, IF, IP, WB*datasheet pdfdatasheet pdf0.1 mg
    MCA1322GA
    MCA1322C *, E, F, IP, WB*datasheet pdfdatasheet pdf0.25 mg
    MCA1322
    MCA1322FBFdatasheet pdfdatasheet pdf0.5 mg
    MCA1322FB
    MCA1322ELC *, E, F, FN, IP, WB*datasheet pdfdatasheet pdf0.5 mg
    MCA1322EL
    MCA1322PEFdatasheet pdfdatasheet pdf100 Tests
    MCA1322PE
    MCA1322A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA1322A647
    MCA1322A488Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA1322A488
    MCA1322TC *, E, F, IP, WB*datasheet pdfdatasheet pdf25 µg
    MCA1322T
    MCA1322BTFdatasheet pdfdatasheet pdf25 µg
    MCA1322BT
    MCA1322FTFdatasheet pdfdatasheet pdf25 µg
    MCA1322FT
    MCA1322A488TFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA1322A488T
    MCA1322PETFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA1322PET
    MCA1322A647TFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA1322A647T
    MCA1322FAFdatasheet pdfdatasheet pdf50 µg
    MCA1322FA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti Mouse CD204 antibody, clone 2F8 recognizes the murine scavenger receptor class A (SRA), type I and II, also known as CD204. CD204 is expressed by tissue macrophages and functions both as an endocytic receptor for lipoproteins and as an adhesion receptor for macrophages binding to ligand rich tissues e.g. atherosclerotic lesions. Clone 2F8 inhibits the uptake of acetylated low-density lipoproteins and also inhibits divalent cation independent adhesion (Fraser et al. 1993).

       Rat anti Mouse CD204 antibody, clone 2F8 recognizes an epitope within SRA that is polymorphic in the SRA from C57BL/6 mice. Clone 2F8 is therefore unsuitable for use with the C57BL/6 mouse strain (Daugherty et al. 2000).
    • Intended Use
    • Target Species
      Mouse
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Pigyes
      Channel catfishyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Pack Size: 25 Tests/0.25mlPurified IgG conjugated to R. Phycoerythrin (RPE) - liquid
      Pack Size: 100 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG - liquid
      Purified IgG conjugated to Biotin - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Biotin - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Alexa Fluor® 488 - liquid
      Pack Size: 25 Tests/0.25mlPurified IgG conjugated to R. Phycoerythrin (RPE) - liquid
      Pack Size: 100 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Purified IgG conjugated to Alexa Fluor® 488 - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
    • Reconstitution
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      None present
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Raw 264 cell line.
    • Purity
    • Approx. Protein Concentrations
      Pack Size: 0.25 mg, 25 µgIgG concentration 1.0 mg/ml
      Pack Size: 0.1 mgIgG concentration 1 mg/ml
      IgG concentration 0.1 mg/ml
      Pack Size: 0.1 mg, 50 µg, 25 µgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      IgG concentration 0.1 mg/ml
      Pack Size: 0.1 mg, 50 µg, 25 µgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 0.05 mg/ml
      Pack Size: 0.25 mg, 25 µgIgG concentration 1.0 mg/ml
      Pack Size: 0.1 mgIgG concentration 1 mg/ml
      Pack Size: 0.1 mg, 50 µg, 25 µgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      Pack Size: 0.25 mg, 25 µgIgG concentration 1.0 mg/ml
      Pack Size: 0.1 mgIgG concentration 1 mg/ml
      Pack Size: 0.1 mg, 50 µg, 25 µgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.05 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised AO rats were fused with cells of the Y3 rat myeloma cell line.
    • Storage
      Pack Size: 25 Tests/0.25mlStore at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 100 TestsPrior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 25 Tests/0.25mlStore at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 100 TestsPrior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -20oC only.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      plasma lipoprotein particle clearance
      low-density lipoprotein particle
      scavenger receptor activity
      receptor-mediated endocytosis
      low-density lipoprotein particle binding
      protein binding
      positive regulation of cholesterol storage
      cholesterol transport
      integral to membrane
      lipoprotein transport
      positive regulation of macrophage derived foam cell differentiation
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry1/501/100
      Immunohistology - Paraffin
      Immunohistology - Resin
      Immunoprecipitation
      Immunohistology - Frozen(1)
      Western Blotting(2)
      Immunofluorescence
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
      (2)
      This product recognises CD204 in J774 cells under non-reduced conditions only.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
      Immunohistology - Paraffin
      Immunohistology - Resin
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/10
      NeatPack Size: 0.1 mg, 25 µg
      1/50Pack Size: 0.5 mg
      Immunohistology - Paraffin
      Immunohistology - Resin
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
      Immunohistology - Paraffin
      Immunohistology - Resin
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/10
      NeatPack Size: 0.1 mg, 25 µg
      1/50Pack Size: 0.5 mg
      Immunohistology - Paraffin
      Immunohistology - Resin
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry1/501/100
      Immunohistology - Paraffin
      Immunohistology - Resin
      Immunoprecipitation
      Immunohistology - Frozen(1)
      Western Blotting(2)
      Immunofluorescence
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
      (2)
      This product recognises CD204 in J774 cells under non-reduced conditions only.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/10
      NeatPack Size: 0.1 mg, 25 µg
      1/50Pack Size: 0.5 mg
      Immunohistology - Paraffin
      Immunohistology - Resin
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry1/501/100
      Immunohistology - Paraffin
      Immunohistology - Resin
      Immunoprecipitation
      Immunohistology - Frozen(1)
      Western Blotting(2)
      Immunofluorescence
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
      (2)
      This product recognises CD204 in J774 cells under non-reduced conditions only.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/10
      NeatPack Size: 0.1 mg, 25 µg
      1/50Pack Size: 0.5 mg
      Immunohistology - Paraffin
      Immunohistology - Resin
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry1/501/100
      Functional Assays
      Immunohistology - Paraffin
      Immunohistology - Resin
      Immunoprecipitation
      Immunohistology - Frozen(1)
      Western Blotting(2)
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
      (2)
      This product recognises CD204 in J774 cells under non-reduced conditions only.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD204 Antibody Formats

    Formats Clone Applications Sizes available
    CD204 Antibody : Alexa Fluor® 488 2F8 F 100 Tests/1ml | 25 Tests/0.25ml
    CD204 Antibody : FITC 2F8 F 50 µg | 25 µg | 0.5 mg | 0.1 mg
    CD204 Antibody : Biotin 2F8 F 0.1 mg | 25 µg
    CD204 Antibody : Low Endotoxin 2F8 C *, E, F, FN, IP, WB* 0.5 mg
    CD204 Antibody : RPE 2F8 F 100 Tests | 25 Tests/0.25ml
    CD204 Antibody : Alexa Fluor® 647 2F8 F 25 Tests/0.25ml | 100 Tests/1ml
    CD204 Antibody : Purified 2F8 C *, E, F, IF, IP, WB* 0.1 mg | 25 µg | 0.25 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
      STAR131A
      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      STAR131B
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      STAR71D549GA
      Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
      STAR71D649GA
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      STAR16D800GA
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
      STAR71D800GA
      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      STAR69
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      STAR17B
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      STAR72
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      STAR21B
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
      STAR73
      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
      STAR20A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
      STAR131A
      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      STAR131B
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      STAR71D549GA
      Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
      STAR71D649GA
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      STAR16D800GA
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
      STAR71D800GA
      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      STAR69
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      STAR17B
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      STAR72
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      STAR21B
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
      STAR73
      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
      STAR20A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
      STAR131A
      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      STAR131B
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      STAR71D549GA
      Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
      STAR71D649GA
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      STAR16D800GA
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
      STAR71D800GA
      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      STAR69
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      STAR17B
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      STAR72
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      STAR21B
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
      STAR73
      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
      STAR20A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
      STAR131A
      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      STAR131B
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      STAR71D549GA
      Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
      STAR71D649GA
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      STAR16D800GA
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
      STAR71D800GA
      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      STAR69
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      STAR17B
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      STAR72
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      STAR21B
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
      STAR73
      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
      STAR20A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B

          Recommended Positive Controls

            Histology Controls

              References

              Write your review

              You may also be interested in...