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CD197 Antibody

CD197 Antibody gallery image 1

Paraffin embedded mouse spleen stained with Goat anti mouse CD197 (AAM70)

CD197 Antibody gallery image 2

Paraffin embedded human spleen stained with Goat anti mouse CD197 (AAM70)

  • CD197 Antibody thumbnail image 1
  • CD197 Antibody thumbnail image 2
  • Goat anti Mouse CD197
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AAM70E, F, FN, P, WBdatasheet pdfdatasheet pdf50 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Goat anti Mouse CD197 antibody recognizes mouse C-C chemokine receptor type 7, also known as CD197, CCR7, Epstein-Barr virus-induced G-protein coupled receptor 1 or MIP-3 beta receptor. CD197 is a 378 amino acid ~43 kDa multi pass type III transmembrane glycoprotein and member of the G-protein coupled receptor 1 family (UniProt: P74447). CD197 is expressed by various lymphoid tissues, activated B and T cells, naive T cells, NK cells and dendritic cells (DCs), which acts a high-affinity receptor for the chemokines MIP-3beta (CCL19) and CCL21.

      Ligand binding of CD197 plays a vital role in the homing of naive T cells and antigen-loaded DCs into secondary organs. Expression of CD197 is strongly up-regulated in B cells infected with Epstein-Barr virus (EBV) and T cells infected with herpesvirus 6 or 7. Goat anti Mouse CD197 antibody can be used in research studies to distinguish between central memory and effector memory T cells and is reported as suitable for use in immunocytochemistry.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to mouse CD197 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.1% Sodium Azide, 0.1% Bovine Serum Albumin.
    • Immunogen
      Synthetic peptide DPGKPRKNVLVVALLVIFQVC corresponding to amino acids 2-22 of mouse CD197.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      inflammatory response
      integral to plasma membrane
      immune response
      C-C chemokine receptor activity
      external side of plasma membrane
      elevation of cytosolic calcium ion concentration
      integral to membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/10
      Functional Assays8ug
      Immunohistology - Paraffin1/250
      Western Blotting1/1000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
      This product is suitable for use in indirect ELISA applications.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD197 Antibody Formats

    Formats Applications Sizes available
    CD197 Antibody : Purified E, F, FN, P, WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • CD197 Antibody thumbnail image 1
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