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CD16/CD32 antibody | FCR4G8

Rat anti Mouse CD16/CD32

Product Type
Monoclonal Antibody
Clone
FCR4G8
Isotype
IgG2b
Specificity
CD16/CD32

Product Code Applications Pack Size List Price Your Price Qty
MCA2305
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.25 mg loader
List Price Your Price
loader
MCA2305GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader

Rat anti Mouse CD16/CD32 antibody, clone FCR4G8 recognizes an epitope expressed by the murine low affinity Fc receptors, Fc gamma III (CD16) and Fc gamma II (CD32). In the mouse only the transmembrane form of CD16 is reported to exist, which is expressed on macrophages, NK cells, granulocytes, myeloid precursors, and subpopulations of T lymphocytes. CD32 is primarily expressed on cells of the myeloid lineage and also on mature B lymphocytes.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
PU5 1.8 IOE7 Balb/c mouse cell line.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cells from immunised rats were fused with cells of the mouse P3X63-Ag8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/50
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

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Description Rabbit F(ab')2 anti Rat IgG:HRP
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Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
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Description Goat anti Rat IgG:DyLight®550 (Mouse Adsorbed)
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Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
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Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)

References for CD16/CD32 antibody

  1. Hart, R. & Greaves, D.R. (2010) Chemerin contributes to inflammation by promoting macrophage adhesion to VCAM-1 and fibronectin through clustering of VLA-4 and VLA-5.
    J Immunol. 185 (6): 3728-39.
  2. da Silva, R.G. et al. (2010) Endothelial alpha3beta1-integrin represses pathological angiogenesis and sustains endothelial-VEGF.
    Am J Pathol. 177 (3): 1534-48.
  3. Birjandi, S.Z. et al. (2011) Alterations in marginal zone macrophages and marginal zone B cells in old mice.
    J Immunol. 186 (6): 3441-51.
  4. Lunnon, K. et al. (2011) Systemic inflammation modulates Fc receptor expression on microglia during chronic neurodegeneration.
    J Immunol. 186 (12): 7215-24.
  5. Birjandi, S.Z. et al. (2011) Alterations in marginal zone macrophages and marginal zone B cells in old mice.
    J Immunol. 186: 3441-51.
  6. Hart, A.D. et al. (2012) Age related changes in microglial phenotype vary between CNS regions: grey versus white matter differences.
    Brain Behav Immun. 26 (5): 754-65.
  7. Teeling, J.L. et al. (2012) Intracerebral immune complex formation induces inflammation in the brain that depends on Fc receptor interaction.
    Acta Neuropathol. 124 (4): 479-90.
  8. Jones, D.T. et al. (2013) Endogenous ribosomal protein L29 (RPL29): a newly identified regulator of angiogenesis in mice.
    Dis Model Mech. 6: 115-24.
  9. View The Latest Product References
  10. Murinello, S. et al. (2014) Fcγ receptor upregulation is associated with immune complex inflammation in the mouse retina and early age-related macular degeneration.
    Invest Ophthalmol Vis Sci. 55 (1): 247-58.
  11. Kapellos, T.S. et al. (2016) A novel real time imaging platform to quantify macrophage phagocytosis.
    Biochem Pharmacol. 116: 107-19.
  12. Ahmed, N. et al. (2022) Blood stem cell PU.1 upregulation is a consequence of differentiation without fast autoregulation.
    J Exp Med. 219 (1): e20202490.

Synonyms
Mouse Seroblock FcR
RRID
AB_2103731
UniProt
P08508
P08101
Entrez Gene
Fcgr2b
Fcgr3
GO Terms
GO:0005886 plasma membrane
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001805 positive regulation of type III hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0001820 serotonin secretion
GO:0016021 integral to membrane
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0007166 cell surface receptor linked signaling pathway
GO:0019770 IgG receptor activity
GO:0030593 neutrophil chemotaxis
GO:0042535 positive regulation of tumor necrosis factor biosynthetic process
GO:0042590 antigen processing and presentation of exogenous peptide antigen via MHC class I
GO:0045576 mast cell activation
GO:0050766 positive regulation of phagocytosis

MCA2305

164036 1806

MCA2305GA

0912 0912R 164441

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