CD11b Antibody | M1/70.15

//www.bio-rad-antibodies.com/mouse-cd11b-antibody-m1-70-15-mca74g.html?RenderPageSpecific=1
CD11b Antibody | M1/70.15 gallery image 1

Figure A. FITC conjugated hamster anti mouse CD11c (MCA1369F) and Alexa647® conjugated rat IgG2b isotype control (MCA6006A647). Figure B. FITC conjugated hamster anti mouse CD11c (MCA1369F) and AlexaFluor647® conjugated rat anti mouse CD11b (MCA74A647). All experiments performed on murine bone marrow in the presence of murine SeroBlock (BUF041A).

Enlarge
CD11b Antibody | M1/70.15 gallery image 2

Published customer image:
Rat anti MouseCD11b antibody, clone M1/70.15 used to identify murine microglia in vitro by immunofluorescence.
Image caption:
Immunocytochemical characterisation of cultures of mouse microglia. Cultures of microglia were immunostained with anti-CD11b (a) and anti-GFAP (c). Cultures of astrocytes were immunostained with anti-CD11b (c) and anti-GFAP (d). All cells were also counterstained with DAPI (blue) to identify the cells' nuclei.

From: Ferger AI, Campanelli L, Reimer V, Muth KN, Merdian I, Ludolph AC, Witting A.
Effects of mitochondrial dysfunction on the immunological properties of microglia.
J Neuroinflammation. 2010 Aug 11;7:45.

Enlarge
CD11b Antibody | M1/70.15 gallery image 3

Published customer image:
Rat anti Mouse CD11b antibody, clone M1/70.15 (MCA74G) used for the localization of microglia in murine brain tissue sections by immunofluorescence
Image caption:
HCT1026 inhibits MPTP-induced increased reactive Mac-1+ microglia in striatum and SNpc. Ageing (9-11 month-old) C57Bl/6 mice fed with a control (ct) or HCT1026 diets (30 mg kg-1) starting at -10 d, underwent an MPTP treatment, as described. At different time-intervals mice were anesthetized and rapidly perfused, the brains processed for immunohistochemistry. Coronal sections at the level of the striatum and SNpc were stained with Mac-1-Ab to localize microglial cells. A-B: Reactive (ameboid-like) microglial cells were counted at different time-intervals after saline and MPTP injection (n = 4/experimental group) in mice fed with a ct or HCT1026 diets, within both Str (A-G) and SNpc (H-N). Differences were analyzed by ANOVA followed by Newman-Keuls test, and considered significant when p <0.05. **p <0.05 vs saline; *° p <0.05 vs MPTP mice fed with ct diet. B-E: Representative confocal images of Mac-1 staining (green) in saline (B), 3 d after MPTP in mice fed with ct diet (C, 20× and D, 40×) or HCT1026 (F, 20× and G, 40×). Insets (100×) show microglia morphologic appearance. I-N: Representative confocal images of Mac-1 staining (red) in SNpc. Note the high density of reactive Mac-1+ cells with rounded cell bodies and short, thick processes 3 d after MPTP administration in mice fed with a ct diet (J-K), as compared to Mac-1 microglia of mice fed with HCT1026 (M-N), exhibiting a more elongated cell body and long ramified processes.

From: L'Episcopo F, Tirolo C, Caniglia S, Testa N, Serra PA, Impagnatiello F, Morale MC, Marchetti B.
Combining nitric oxide release with anti-inflammatory activity preserves nigrostriatal dopaminergic innervation and prevents motor impairment in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine model of Parkinson's disease.
J Neuroinflammation. 2010 Nov 23;7:83.

Enlarge
CD11b Antibody | M1/70.15 gallery image 4

Published customer image:
Rat anti Mouse CD11b antibody, clone M1/70.15 (MCA74G) used for the localization of microglia in murine brain tissue sections by immunofluorescence
Image caption:
HCT1026 inhibits MPTP-induced increased Mac-1, PHOX, iNOX and 3-Nitrotyrosine expression in SNpc. A-B: Western blotting of phagocyte oxidase PHOX (A) and Mac-1 (B) within the VM at different time-intervals after saline or MPTP injection in mice fed with the control or HCT1026 diet. The data from experimental bands were normalized to β-actin, before statistical analysis of variance. Values are expressed as % of saline-injected controls. Differences were analyzed by ANOVA followed by Newman-Keuls test, and considered significant when p <0.05. * p <0.05 compared to saline; ° p <0.05 vs MPTP fed with the control diet. C. Semi-quantitative RT-PCR for iNOS. The 250 ng of cDNA were used for PCR, by using Super Taq DNA polymerase with specific primer pairs for iNOS (500 bp) and Classic GADPH Standard (270 bp). Samples from PCR reactions were processed as described. Fluorescent bands of amplified gene products were analyzed, the values normalized against GADPH and ratios expressed as % of control, within each experimental group (C), see text for details. Differences were analyzed by ANOVA as above. ** p <0.05 vs saline; ° p <0.05 vs MPTP + control diet. D: Mean numbers of Mac1+iNOS+ cells within the SNpc in saline and MPTP mice fed with the control or HCT1026 diet. Cell counts obtained as described in Material section. E-F: Representative confocal images showing double staining with Mac-1 (green) and iNOS (red) in MPTP mice fed with the control (E) or HCT1026 (F) diets. G: Percent (%) of TH+ neurons colocalizing with 3-nitrotyrosine (3-NT), a peroxynitrite footprint. Dual stained TH+ 3-NT+ neurons were counted as described and values expressed as % of total TH+ neurons. H-I: Representative confocal images showing dual immunostaining with 3-NT (green) and TH (red) in MPTP mice fed with a ct diet (H) showing that a large proportion of DAergic neurons colocalize (orange-to-yellow) as opposed to TH neurons of mice fed with HCT1026 (E) where no colocalization was observed in the large part of SNpc neurons.

From: L'Episcopo F, Tirolo C, Caniglia S, Testa N, Serra PA, Impagnatiello F, Morale MC, Marchetti B.
Combining nitric oxide release with anti-inflammatory activity preserves nigrostriatal dopaminergic innervation and prevents motor impairment in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine model of Parkinson's disease.
J Neuroinflammation. 2010 Nov 23;7:83.

Enlarge
CD11b Antibody | M1/70.15 gallery image 5

Published customer image:
Rat anti mouse CD11b antibody, clone M1/70.15 (MCA74G) used for the assessment of CD11b expression by activated N9 microglial cells using immunofluorescence.
Image caption:
Localization of CD11b and p-STAT3 immunoreactivity in activated N9 cells. Experiments were performed as described above. Confocal immunofluorescence microscopy was performed on cultures that were immunoreacted with antibodies against phospho-STAT3 tyr705 (left, rabbit monoclonal, 1:100; FITC-conjugated sheep anti- rabbit secondary, 1:200) and CD11b (right, as Figure 1 shows) at 12 h after EMF exposure (A-L). (A-C) Untreated cultures were used as a control. (D-F) Cultures pretreated with P6 (10 μM). (G-I) EMF induced more phosphorylation of STAT3 (green) and expression of CD11b (red) in N9 cells. (J-L) P6 inhibits the phosphorylation of STAT3 and expression of CD11b at 12 h after EMF exposure. (M-O) P6 pretreatment significantly suppresses the phosphorylation of STAT3, but a slight and significant increase of CD11b is still apparent at 3 h after EMF exposure. Scale bar 10 μm. (P) Bar graphs show semi-quantification of fluorescence intensity for p-STAT3 and CD11b in N9 cells. Results are presented as mean ± S.D. of three independent experiments. Statistical comparisons to control are indicated by * p <0.05; ** p <0.01.

From: Yang X, He G, Hao Y, Chen C, Li M, Wang Y, Zhang G, Yu Z.
The role of the JAK2-STAT3 pathway in pro-inflammatory responses of EMF-stimulated N9 microglial cells.
J Neuroinflammation. 2010 Sep 9;7:54.

Enlarge
CD11b Antibody | M1/70.15 gallery image 6

Immunoperoxidase staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody (MCA74) followed by horseradish peroxidase conjugated Goat anti Rat IgG (STAR72). Low power

Enlarge
CD11b Antibody | M1/70.15 gallery image 7

Published customer image:
AlexaFluor®646 conjugated Rat ati mouse CD11b antibody, clone M1/70.15 used for the evaluation of CD11b expression on immortalized murine microglia by flow cytometry.expression
Image caption:
IMG cells display similar morphology to primary microglia and express the microglia markers CD11b and F4/80. a Representative DIC images of IMG, BV-2, and primary adult microglial cells. Images are at ×40 magnification. b Flow cytometry of IMG cells. Representative zebra plot (left panel) of IMG showing a population of >95 % was gated for the analysis of IMG F4/80 (middle panel) and CD11b (right panel) expression (filled trace) by flow cytometry. Isotype controls (open trace) are also included in the graphs

From: McCarthy RC, Lu DY, Alkhateeb A, Gardeck AM, Lee CH, Wessling-Resnick M.
Characterization of a novel adult murine immortalized microglial cell line and its activation by amyloid-beta.
J Neuroinflammation. 2016 Jan 27;13:21.

Enlarge
CD11b Antibody | M1/70.15 gallery image 8

Immunoperoxidase staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody (MCA74) followed by horseradish peroxidase conjugated Goat anti Rat IgG (STAR72). Medium power

Enlarge
CD11b Antibody | M1/70.15 gallery image 9

Published customer image:
Rat anti Mouse CD11b antibody, clone M1/70.15 (MCA74G) used for identification of CD11b expressing cells in murine sciatic nerves by immunofluorescence on cryosections.
Image caption:
Overexpression of ERp57 in transgenic mice increases axonal regeneration after peripheral nerve injury.
(A) Non-Tg and Tg-ERp57 mice were damaged and sciatic nerves were extracted at 7, 11 and 14 days post-injury (dpi). Transversal semi-thin sections were obtained from the distal region and remyelinated axons (white arrows) and degenerated myelins (black arrows) were analyzed. (B) Quantification of remyelinated axons and (C) degenerated myelin density was measured at 7, 11 and 14 days post-injury. (D) Tg- ERp57 and Non-Tg sciatic nerves were processed for immunofluorescence in uninjured conditions and at 14 dpi. Sciatic nerves were analyzed for MBP (red), Cd11b (green) and nuclei were stained using DAPI (blue). (E) The staining density for Cd11b was quantified 14 days after injury (right panel). Data is presented as mean ± S.E.M. * p <0.05, ** p < 0.01, *** p <0.001. For histological analysis, statistical differences were obtained using a student's t-test (n = 3 animals per group). Scale bar: 20μm.

From: Castillo V, Oñate M, Woehlbier U, Rozas P, Andreu C, Medinas D, et al. (2015)
Functional Role of the Disulfide Isomerase ERp57 in Axonal Regeneration.
PLoS ONE 10(9): e0136620.

Enlarge
CD11b Antibody | M1/70.15 gallery image 10

Immunoperoxidase staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody (MCA74) followed by horseradish peroxidase conjugated Goat anti Rat IgG (STAR72). High power

Enlarge
CD11b Antibody | M1/70.15 gallery image 11

Published customer image:
Rat anti Mouse CD11b antibody, clone M1/70.15 (MCA74G) used for identification of CD11b expressing cells in murine sciatic nerves by immunofluorescence on cryosections.
Image caption:
ERp57 overexpression reduces axonal degeneration.
(A) Electron microscopy of Non-Tg and Tg-ERp57 uninjured and 14 days-damaged nerves. In uninjured conditions white arrowheads indicate axoplasm of myelinated fibers, black arrowheads, compact myelin sheaths and asterisks, unmyelinated fibers. At 14 days post-injury black arrows indicated degenerated myelins and white arrows, remyelinated axons. Scale bar: 4μm. (B) hERp57 mRNA levels were determined in macrophages isolated from alveoli of Non-Tg and Tg-ERp57 mice after cell sorting of Cd11b-positive cells using real-time PCR (n = 2 per group). Cortex tissue from these animals was used as positive control. (C) Non-Tg and Tg-ERp57 mice were damaged and sciatic nerves were extracted at 14 days post-injury. Contralateral uninjured nerves were used as control. Transversal slides were processed for immunofluorescence for FLAG (red), MBP (green) and DAPI (blue) to identify Schwann cells. (D) Animals described in C were used for immunofluorescence to stain FLAG (red), Cd11b (green) and DAPI (blue) to analyse the infiltrating macrophage population. At the right panels, magnifications of indicated areas are shown. Scale bar: 20 mm. In B data are shown as mean.

From: Castillo V, Oñate M, Woehlbier U, Rozas P, Andreu C, Medinas D, et al. (2015)
Functional Role of the Disulfide Isomerase ERp57 in Axonal Regeneration.
PLoS ONE 10(9): e0136620.

Enlarge
CD11b Antibody | M1/70.15 gallery image 12

Immunofluorescence staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody (MCA74), green in A and Rat anti Mouse CD8 antibody (MCA1108), red in B, Merged image in C

Enlarge
  • CD11b Antibody | M1/70.15 thumbnail image 1
  • CD11b Antibody | M1/70.15 thumbnail image 2
  • CD11b Antibody | M1/70.15 thumbnail image 3
  • CD11b Antibody | M1/70.15 thumbnail image 4
  • CD11b Antibody | M1/70.15 thumbnail image 5
  • CD11b Antibody | M1/70.15 thumbnail image 6
  • CD11b Antibody | M1/70.15 thumbnail image 7
  • CD11b Antibody | M1/70.15 thumbnail image 8
  • CD11b Antibody | M1/70.15 thumbnail image 9
  • CD11b Antibody | M1/70.15 thumbnail image 10
  • CD11b Antibody | M1/70.15 thumbnail image 11
  • CD11b Antibody | M1/70.15 thumbnail image 12
  • Rat anti Mouse CD11b:Alexa Fluor® 488
  • Rat anti Mouse CD11b:RPE-Alexa Fluor® 750
  • Rat anti Mouse CD11b:FITC
  • Rat anti Mouse CD11b:Biotin
  • Rat anti Mouse CD11b:RPE-Cy5
  • Rat anti Mouse CD11b:RPE-Alexa Fluor® 750
  • Rat anti Mouse CD11b:FITC
  • Rat anti Mouse CD11b:RPE
  • Rat anti Mouse CD11b:RPE
  • Rat anti Mouse CD11b:FITC
  • Rat anti Mouse CD11b:Alexa Fluor® 647
  • Rat anti Mouse CD11b:RPE-Alexa Fluor® 647
  • Rat anti Mouse CD11b
  • Rat anti Mouse CD11b
  • Rat anti Mouse CD11b:Biotin
  • Rat anti Mouse CD11b:Alexa Fluor® 647
  • Rat anti Mouse CD11b:Alexa Fluor® 700
  • Rat anti Mouse CD11b:RPE-Alexa Fluor® 647
  • Rat anti Mouse CD11b:FITC
  • Rat anti Mouse CD11b:Low Endotoxin
  • Rat anti Mouse CD11b:RPE
  • Rat anti Mouse CD11b:Alexa Fluor® 488
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    M1/70.15
  • Isotype
    IgG2b
11 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA74FFdatasheet pdfdatasheet pdf0.1 mg
    MCA74F
    MCA74GAC, F, IF, IP, P *datasheet pdfdatasheet pdf0.1 mg
    MCA74GA
    MCA74BFdatasheet pdfdatasheet pdf0.1 mg
    MCA74B
    MCA74GC, F, IF, IP, P *datasheet pdfdatasheet pdf0.25 mg
    MCA74G
    MCA74FBFdatasheet pdfdatasheet pdf0.5 mg
    MCA74FB
    MCA74ELC, F, FN, IF, IP, P *datasheet pdfdatasheet pdf0.5 mg
    MCA74EL
    MCA74PEFdatasheet pdfdatasheet pdf100 Tests
    MCA74PE
    MCA74CFdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA74C
    MCA74P750Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA74P750
    MCA74P647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA74P647
    MCA74A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA74A647
    MCA74A700Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA74A700
    MCA74A488Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA74A488
    MCA74BTFdatasheet pdfdatasheet pdf25 µg
    MCA74BT
    MCA74FTFdatasheet pdfdatasheet pdf25 µg
    MCA74FT
    MCA74PETFdatasheet pdfdatasheet pdf25 Tests
    MCA74PET
    MCA74P647TFdatasheet pdfdatasheet pdf25 Tests
    MCA74P647T
    MCA74A488TFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA74A488T
    MCA74P750TFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA74P750T
    MCA74A647TFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA74A647T
    MCA74FAFdatasheet pdfdatasheet pdf50 µg
    MCA74FA
    MCA74PEBFdatasheet pdfdatasheet pdf500 Tests
    MCA74PEB
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti Mouse CD11b antibody, clone M1/70.15 recognizes the murine CD11b cell surface antigen also known as the alpha M integrin chain or MAC-1, a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages.

      The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.

      Rat anti Mouse CD11b antibody, clone M1/70.15 has been reported to block iC3b binding to its receptor (Beller et al. 1982).

      Rat anti Mouse CD11b antibody, clone M1/70.15 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue (Whiteland et al. 1995).

      This product is routinely tested in flow cytometry on mouse peritoneal macrophages.
    • Intended Use
    • Target Species
      Mouse
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Humanyes
      Rabbityes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Alexa Fluor® 488 - liquid
      Pack Size: 100 Tests/1mlPurified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 750 - lyophilised
      Pack Size: 25 Tests/0.25mlPurified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 750 - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Biotin - liquid
      Purified IgG conjugated to R. Phycoerythrin -Cy5 (RPE- Cy5) - liquid
      Pack Size: 100 Tests/1mlPurified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 750 - lyophilised
      Pack Size: 25 Tests/0.25mlPurified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 750 - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Pack Size: 500 Tests, 100 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Pack Size: 25 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - liquid
      Pack Size: 500 Tests, 100 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Pack Size: 25 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 647 - lyophilised
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Biotin - liquid
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Purified IgG conjugated to Alexa Fluor® 700 - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 647 - lyophilised
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Pack Size: 500 Tests, 100 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Pack Size: 25 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - liquid
      Purified IgG conjugated to Alexa Fluor® 488 - liquid
    • Reconstitution
      Pack Size: 100 Tests/1mlReconstitute with 1.0 ml distilled water
      Pack Size: 100 Tests/1mlReconstitute with 1.0 ml distilled water
      Pack Size: 500 TestsReconstitute with 5.0 ml distilled water
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 500 TestsReconstitute with 5.0 ml distilled water
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
      Pack Size: 100 Tests/1mlReconstitute with 1.0 ml distilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
      Pack Size: 100 Tests/1mlReconstitute with 1.0 ml distilled water
      Pack Size: 500 TestsReconstitute with 5.0 ml distilled water
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.1 mg, 50 µg, 25 µgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Pack Size: 0.5 mgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared from ascites or tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.1 mg, 50 µg, 25 µgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Pack Size: 0.5 mgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.1 mg, 50 µg, 25 µgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Pack Size: 0.5 mgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Pack Size: 0.1 mgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.25 mgPurified IgG prepared by ion exchange chromatography
      Pack Size: 0.1 mgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.25 mgPurified IgG prepared by ion exchange chromatography
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Pack Size: 0.1 mg, 50 µg, 25 µgPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Pack Size: 0.5 mgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.1% Sodium Azide (NaN3)
      Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      None present
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      T cell enriched splenocytes from B10 mice.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.05 mg/ml
      Pack Size: 0.1 mg, 50 µg, 25 µgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      IgG concentration 0.1 mg/ml
      Pack Size: 0.1 mg, 50 µg, 25 µgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      Pack Size: 0.1 mg, 50 µg, 25 µgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 0.05 mg/ml
      Pack Size: 0.1 mg, 50 µg, 25 µgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.05 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised DA rats were fused with cells of the NS1/1.Ag4.1 mouse myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 500 Tests, 100 TestsPrior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 25 TestsStore at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Pack Size: 500 Tests, 100 TestsPrior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 25 TestsStore at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted.

      This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted.

      This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -20oC only.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 500 Tests, 100 TestsPrior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 25 TestsStore at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      12 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      6 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
      12 months from date of reconstitution.
      12 months from date of despatch
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
    • GO Terms
      leukocyte cell-cell adhesion
      heparan sulfate proteoglycan binding
      receptor activity
      external side of plasma membrane
      heparin binding
      integrin complex
      activated T cell proliferation
      integrin-mediated signaling pathway
      opsonin binding
      neutrophil chemotaxis
      cellular extravasation
      nucleus
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
      Cy® and CyDye® are registered trademarks of GE Healthcare
      This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
      1/10Pack Size: 0.1 mg, 25 µg
      1/100Pack Size: 0.5 mg
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
      1/10Pack Size: 0.1 mg, 25 µg
      1/100Pack Size: 0.5 mg
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
      1/10Pack Size: 0.1 mg, 25 µg
      1/100Pack Size: 0.5 mg
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/50
      1/50Pack Size: 0.25 mg
      1/200Pack Size: 0.1 mg
      Immunofluorescence
      Immunohistology - Frozen
      Immunoprecipitation
      Immunohistology - Paraffin(1)
      (1)
      PLP fixation is recommended for optimal results, see Whiteland et al. for details
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/50
      1/50Pack Size: 0.25 mg
      1/200Pack Size: 0.1 mg
      Immunofluorescence
      Immunohistology - Frozen
      Immunoprecipitation
      Immunohistology - Paraffin(1)
      (1)
      PLP fixation is recommended for optimal results, see Whiteland et al. for details
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
      1/10Pack Size: 0.1 mg, 25 µg
      1/100Pack Size: 0.5 mg
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Functional Assays
      Immunofluorescence
      Immunohistology - Frozen
      Immunoprecipitation
      Immunohistology - Paraffin(1)
      (1)
      PLP fixation is recommended for optimal results, see Whiteland et al. for details
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD11b Antibody Formats

    Formats Clone Applications Sizes available
    CD11b Antibody : RPE-Alexa Fluor® 647 M1/70.15 F 100 Tests/1ml | 25 Tests
    CD11b Antibody : Alexa Fluor® 488 M1/70.15 F 100 Tests/1ml | 25 Tests/0.25ml
    CD11b Antibody : Purified M1/70.15 C, F, IF, IP, P * 0.1 mg | 0.25 mg
    CD11b Antibody : RPE-Cy5 M1/70.15 F 100 Tests/1ml
    CD11b Antibody : Low Endotoxin M1/70.15 C, F, FN, IF, IP, P * 0.5 mg
    CD11b Antibody : RPE M1/70.15 F 100 Tests | 500 Tests | 25 Tests
    CD11b Antibody : Biotin M1/70.15 F 0.1 mg | 25 µg
    CD11b Antibody : Alexa Fluor® 700 M1/70.15 F 100 Tests/1ml
    CD11b Antibody : RPE-Alexa Fluor® 750 M1/70.15 F 25 Tests/0.25ml | 100 Tests/1ml
    CD11b Antibody : FITC M1/70.15 F 0.1 mg | 50 µg | 0.5 mg | 25 µg
    CD11b Antibody : Alexa Fluor® 647 M1/70.15 F 25 Tests/0.25ml | 100 Tests/1ml
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
      STAR131A
      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      STAR131B
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      STAR71D549GA
      Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
      STAR71D649GA
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      STAR16D800GA
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
      STAR71D800GA
      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      STAR69
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      STAR17B
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      STAR72
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      STAR21B
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
      STAR73
      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
      STAR20A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
      STAR131A
      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      STAR131B
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      STAR71D549GA
      Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
      STAR71D649GA
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      STAR16D800GA
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
      STAR71D800GA
      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      STAR69
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      STAR17B
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      STAR72
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      STAR21B
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
      STAR73
      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
      STAR20A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
      STAR131A
      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      STAR131B
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      STAR71D549GA
      Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
      STAR71D649GA
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      STAR16D800GA
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
      STAR71D800GA
      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      STAR69
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      STAR17B
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      STAR72
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      STAR21B
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
      STAR73
      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
      STAR20A

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2b Negative Control:RPE-Alexa Fluor® 750MCA6006P750100 Tests/1mlF
        MCA6006P750
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2b Negative Control:RPE-Alexa Fluor® 750MCA6006P750100 Tests/1mlF
        MCA6006P750
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2b Negative Control:Alexa Fluor® 700MCA1125A700100 Tests/1mlF
        MCA1125A700

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              Source Reference

              Antibody Characterization Reference

              References

              Write your review

              You may also be interested in...