CD102 Antibody | mIC2/4 (3C4)

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CD102 Antibody | mIC2/4 (3C4) gallery image 1

Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD102 (MCA2295)

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CD102 Antibody | mIC2/4 (3C4) gallery image 2

Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD102 (MCA2295GA)

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CD102 Antibody | mIC2/4 (3C4) gallery image 3

Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD102: Biotin (MCA2295B)

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CD102 Antibody | mIC2/4 (3C4) gallery image 4

Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD102: FITC (MCA2295F)

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CD102 Antibody | mIC2/4 (3C4) gallery image 5

Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD102: FITC (MCA2295FB)

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CD102 Antibody | mIC2/4 (3C4) gallery image 6

Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD102: Low Endotoxin (MCA2295EL)

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CD102 Antibody | mIC2/4 (3C4) gallery image 7

Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD102: RPE (MCA2295PE)

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CD102 Antibody | mIC2/4 (3C4) gallery image 8

Staining of mouse spleen cells with Rat anti Mouse Beta CD102 : Alexa Fluor® 488 (MCA2295A488)

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CD102 Antibody | mIC2/4 (3C4) gallery image 9

Staining of mouse spleen cells with Rat anti Mouse CD102 : Alexa Fluor® 647 (MCA2295A647)

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  • Rat anti Mouse CD102:Low Endotoxin
  • Rat anti Mouse CD102
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    mIC2/4 (3C4)
  • Isotype
    IgG2a
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2295F, IPdatasheet pdfdatasheet pdf0.25 mg
    MCA2295
    MCA2295ELF, FN, IPdatasheet pdfdatasheet pdf0.5 mg
    MCA2295EL
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti Mouse CD102 antibody, clone mIC2/4 (3C4) recognizes murine intercellular adhesion molecule-2 (ICAM-2), also known as CD102. Murine CD102 is a glycosylated cell surface protein that is broadly expressed on most leucocytes and is constitutively expressed on endothelial cells. CD102 interacts with the CD11a/CD18 integrin LFA-1. Reports suggest that CD102 may play a role in lymphocyte re-circulation and T-cell activation.

      Rat anti Mouse CD102 antibody, clone mIC2/4 is reported to inhibit interactions between CD102 and LFA-1 (Xuet al. 1996).
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      None present
      0.09%Sodium Azide
    • Immunogen
      COS cells transfected with murine ICAM-2
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Lewis rats were fused with cells of the Mouse P3X63Ag8.653 myeloma cell line.
    • Storage
      Store at -20oC only.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/200
      Functional Assays
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/200
      Immunoprecipitation

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106cells in 100ul.
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD102 Antibody Formats

    Formats Clone Applications Sizes available
    CD102 Antibody : Purified mIC2/4 (3C4) F, IP 0.25 mg
    CD102 Antibody : Low Endotoxin mIC2/4 (3C4) F, FN, IP 0.5 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

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      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
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      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
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      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      STAR16D800GA
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
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      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
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      STAR20A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
      STAR131A
      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      STAR131B
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      STAR71D549GA
      Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
      STAR71D649GA
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      STAR16D800GA
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
      STAR71D800GA
      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      STAR69
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      STAR17B
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      STAR72
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      STAR21B
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2a Negative Control:Low EndotoxinMCA1212EL0.5 mgF
        MCA1212EL
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2a Negative ControlMCA12121 mlE, F
        MCA1212

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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