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Feline Immunodeficiency Virus p24 gag antibody | PAK3-2C1

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Mouse anti Feline Immunodeficiency Virus p24 gag

Product Type
Monoclonal Antibody
Clone
PAK3-2C1
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA2278
Datasheet
C* E F* IF WB
MSDS
0.25 mg loader

Mouse anti Feline Immunodeficiency Virus p24 gag antibody, clone PAK3-2C1 recognizes an epitope on the C-terminal alpha-helix 9 within the amino-acid sequence AEVKLYLKQSLSIAN of feline immunodeficiency virus (FIV) p24 gag.

The lentivirus FIV, responsible for a progressive and debilitating immune deficiency syndrome in domestic cats, similar to that caused by the human Immunodeficiency Virus (HIV), is a complex retrovirus with a tightly-packed genome, containing the structural genes gag, env and pro-pol and the accessory genes vif, rev and ORF-A/2.

The specific binding epitope recognized by Mouse anti Feline Immunodeficiency Virus p24 gag antibody, clone PAK3-2C1 appears to be enclosed in a tight peptide coil during gag production and maturation. The addition of 0.3% Triton-X detergent is recommended to reveal this epitope for antibody binding.

Mouse anti Feline Immunodeficiency Virus p24 gag antibody, clone PAK3-2C1 detects a dominant band of approximately 24kDa, and also detects p24 gag precursor bands at 36, 39, 49 and 52kDa under reducing conditions in Western blotting.

Mouse anti Feline Immunodeficiency Virus p24 gag antibody, clone PAK3-2C1 does not recognize Feline Leukaemia Virus (FeLV), Feline Herpes Virus (FHV) type 1, Feline Coronavirus or Feline Calicivirus.

Product Details

Target Species
Viral
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.05%Sodium Azide
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of Feline Immunodeficiency Virus p24 gag antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1
Immunofluorescence
Immunohistology - Frozen 2
Immunohistology - Paraffin
Western Blotting
  1. 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
  2. 2The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Application Based External Images

Immunofluorescence

Western Blotting

Product Specific References

References for Feline Immunodeficiency Virus p24 gag antibody

  1. Lerner, D.L. et al. (1995) Increased mutation frequency of feline immunodeficiency virus lacking functional deoxyuridine-triphosphatase.
    Proc Natl Acad Sci U S A. 92 (16): 7480-4.
  2. Chatterji, U. et al. (2000) Feline immunodeficiency virus Vif localizes to the nucleus.
    J Virol. 74 (6): 2533-40.
  3. Norway, R.M. et al. (2001) Thymic lesions in cats infected with a pathogenic molecular clone or an ORF-A/2-deficient molecular clone of feline immunodeficiency virus.
    J Virol. 75 (13): 5833-41.
  4. Simon, J.H. et al. (1997) The Vif and Gag proteins of human immunodeficiency virus type 1 colocalize in infected human T cells.
    J Virol. 71 (7): 5259-67.
  5. Rolim, V.M. et al. (2016) Myocarditis caused by Feline Immunodeficiency Virus in Five Cats with Hypertrophic Cardiomyopathy.
    J Comp Pathol. 154 (1): 3-8.
  6. Freer, G. et al. (2007) Effects of feline immunodeficiency virus on feline monocyte-derived dendritic cells infected by spinoculation.
    J Gen Virol. 88 (Pt 9): 2574-82.
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