Feline Immunodeficiency Virus p24 gag antibody | PAK3-2C1
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Mouse anti Feline Immunodeficiency Virus p24 gag
- Product Type
- Monoclonal Antibody
- Clone
- PAK3-2C1
- Isotype
- IgG1
- Specificity
- Feline Immunodeficiency Virus p24 gag
Antibody Quality and Performance Guarantee
| Mouse anti Feline Immunodeficiency Virus p24 gag antibody, clone PAK3-2C1 recognizes an epitope on the C-terminal alpha-helix 9 within the amino-acid sequence AEVKLYLKQSLSIAN of feline immunodeficiency virus (FIV) p24 gag. The lentivirus FIV, responsible for a progressive and debilitating immune deficiency syndrome in domestic cats, similar to that caused by the human Immunodeficiency Virus (HIV), is a complex retrovirus with a tightly-packed genome, containing the structural genes gag, env and pro-pol and the accessory genes vif, rev and ORF-A/2. The specific binding epitope recognized by Mouse anti Feline Immunodeficiency Virus p24 gag antibody, clone PAK3-2C1 appears to be enclosed in a tight peptide coil during gag production and maturation. The addition of 0.3% Triton-X detergent is recommended to reveal this epitope for antibody binding. Mouse anti Feline Immunodeficiency Virus p24 gag antibody, clone PAK3-2C1 detects a dominant band of approximately 24kDa, and also detects p24 gag precursor bands at 36, 39, 49 and 52 kDa under reducing conditions in Western blotting. Mouse anti Feline Immunodeficiency Virus p24 gag antibody, clone PAK3-2C1 does not recognize Feline Leukaemia Virus (FeLV), Feline Herpes Virus (FHV) type 1, Feline Coronavirus or Feline Calicivirus. |
- Target Species
- Viral
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% sodium azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
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| Flow Cytometry 1 | |
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| Immunofluorescence | |
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| Immunohistology - Frozen 2 | |
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| Immunohistology - Paraffin | |||
| Western Blotting | |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
- 2The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
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References for Feline Immunodeficiency Virus p24 gag antibody
-
Lerner, D.L. et al. (1995) Increased mutation frequency of feline immunodeficiency virus lacking functional deoxyuridine-triphosphatase.
Proc Natl Acad Sci U S A. 92 (16): 7480-4. -
Chatterji, U. et al. (2000) Feline immunodeficiency virus Vif localizes to the nucleus.
J Virol. 74 (6): 2533-40. -
Rolim, V.M. et al. (2016) Myocarditis caused by Feline Immunodeficiency Virus in Five Cats with Hypertrophic Cardiomyopathy.
J Comp Pathol. 154 (1): 3-8. -
Freer, G. et al. (2007) Effects of feline immunodeficiency virus on feline monocyte-derived dendritic cells infected by spinoculation.
J Gen Virol. 88 (Pt 9): 2574-82.
- RRID
- AB_2108361
MCA2278
090315
149941
154253
164983
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