Trastuzumab antibody | AbD25279
A microtiter plate was coated over night with human IgG1/kappa, human ErbB2, alemtuzumab or trastuzumab at a concentration of 5 μg/ml. After washing and blocking with PBST+5% BSA, the human ErbB2/trastuzumab complex was formed by adding 2 μg/ml trastuzumab to the wells coated with human ErbB2, detection was performed using Human anti trastuzumab (clone AbD25279) titrated to the given concentrations in PBST+10% human serum, followed by HRP labeled mouse anti His tag (MCA5995P) in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate. Data is presented as the mean of three measurements
A microtiter plate was coated over night with human ErbB2 at a concentration of 5 μg/ml. After washing and blocking with PBST+5% BSA, 10% human serum was added spiked with increasing concentrations of trastuzumab. Detection was performed using human anti trastuzumab (clone AbD25279) at a concentration of 2 μg/ml, followed by HRP labeled mouse anti-His tag antibody (MCA5995P) in HISPEC diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate. Data is presented as the mean of three measurements
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST+5% BSA, detection was performed using Human anti trastuzumab (clone AbD25279) at a concentration of 2 μg/ml, followed by HRP labeled mouse anti DYKDDDDK (MCA4764P) in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate
Filter by Application:
E ResetHuman anti Trastuzumab (Drug/Target Complex)
- Product Type
- Monoclonal Antibody
- Clone
- AbD25279
- Isotype
- HuCAL Fab bivalent
- Specificity
- Trastuzumab
- Region
- DRUG/TARGET COMPLEX
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| Human anti trastuzumab drug/target complex, clone AbD25279, is a bivalent Fab antibody that specifically recognizes trastuzumab when in a complex with its target, the extracellular domain of the human epidermal growth factor receptor 2 (HER2, also known as ErbB2). It does not recognize the free drug or unbound HER2. The antibody can be used in pharmacokinetic (PK) antigen capture format assays to measure trastuzumab that has been captured via immobilized HER2. It is ideal for bioanalytical assays to measure the level of drug or biosimilar in complex with the HER2 target. Trastuzumab (trade name Herceptin®) is a drug used in the treatment of HER2 positive breast cancer and other HER2 overexpressing cancers including HER2-positive metastatic cancers of the gastrointestinal tract. Trastuzumab binds to the HER2 (or c-erbB2) proto-oncogene, an EGF receptor-like protein found on 20-30% of breast cancer cells. The binding leads to antibody mediated (complement mediated) killing of the HER2 positive cells. View a summary of all anti-trastuzumab antibodies |
- Product Form
- A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
- Preparation
- Metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Trastuzumab/ErbB2 complex
- Affinity
- The monovalent intrinsic affinity of this antibody was measured as KD=31 nM by real time, label-free molecular interaction analysis on immobilized trastuzumab/ErbB2 complex.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- This product may be used as a detection reagent in a direct or an indirect ELISA for trastuzumab captured by immobilized human ErbB2.
Protocol: PK antigen capture ELISA to measure bound drug exclusively
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | Hispec Assay Diluent | ||||||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody | Log in | |||
| List Price | Your Price | ||||||
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| Description | LYNX Rapid HRP Antibody Conjugation Kit | ||||||
| Rat anti DYKDDDDK Tag:HRP | MCA4764P | E P WB | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Rat anti DYKDDDDK Tag:HRP | ||||||
| Mouse anti Penta Histidine Tag:HRP | MCA5995P | E WB | 125 µl |
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| List Price | Your Price | ||||||
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| Description | Mouse anti Penta Histidine Tag:HRP | ||||||
References for Trastuzumab antibody
-
Harth, S. et al. (2019) Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics.
MAbs. 11 (1): 178-190.
- Synonyms
- HERCEPTIN®
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
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