Trastuzumab antibody | AbD25279



Human anti Trastuzumab (Drug/Target Complex)
Anti-trastuzumab drug-target complex antibody specifically recognizes the trastuzumab- HER2/neu receptor complex exclusively and detects trastuzumab or biosimilars only when bound to the HER2/neu receptor. This antibody in monovalent Fab format is suitable for a PK antigen capture format assay.
- Product Type
- Monoclonal Antibody
- Clone
- AbD25279
- Isotype
- HuCAL Fab bivalent
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
HCA263 | E | 0.1 mg |
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Trastuzumab (trade name Herceptin®) is a drug used in the treatment of HER2 positive breast cancer and other HER2 overexpressing cancers including HER2-positive metastatic cancers of the gastrointestinal tract. Trastuzumab binds to the HER2 (or c-erbB2) proto-oncogene, an EGF receptor-like protein found on 20-30% of breast cancer cells. The binding leads to antibody mediated (complement mediated) killing of the HER2 positive cells.
View a summary of all anti-trastuzumab antibodies
Product Details
- Product Form
- A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
- Preparation
- Metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Trastuzumab
- Affinity
- The monovalent intrinsic affinity of this antibody was measured as KD=31 nM by real time, label-free molecular interaction analysis on immobilized trastuzumab/ErbB2 complex.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
- Regulatory
- For research purposes only
Applications of Trastuzumab antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA |
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- This product may be used as a detection reagent in a direct or an indirect ELISA for trastuzumab captured by immobilized human ErbB2.
Protocol: PK antigen capture ELISA to measure bound drug exclusively
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody | ||
Rat anti DYKDDDDK Tag:HRP | MCA4764P | E P WB | 0.1 mg |
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Mouse anti Penta Histidine Tag:HRP | MCA5995P | E WB | 125 µl |
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Product Specific References
References for Trastuzumab antibody
-
Harth, S. et al. (2019) Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics.
MAbs. 11 (1): 178-190.
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