Trastuzumab antibody | AbD25279

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Human anti Trastuzumab (Drug/Target Complex)

Anti-trastuzumab drug-target complex antibody specifically recognizes the trastuzumab- HER2/neu receptor complex exclusively and detects trastuzumab or biosimilars only when bound to the HER2/neu receptor. This antibody in monovalent Fab format is suitable for a PK antigen capture format assay.

Product Type
Monoclonal Antibody
Clone
AbD25279
Isotype
HuCAL Fab bivalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA263 E 0.1 mg
Human anti trastuzumab drug/target complex, clone AbD25279, is a bivalent Fab antibody that specifically recognizes trastuzumab when in a complex with its target, the extracellular domain of the human epidermal growth factor receptor 2 (HER2, also known as ErbB2). It does not recognize the free drug or unbound HER2. The antibody can be used in pharmacokinetic (PK) antigen capture format assays to measure trastuzumab that has been captured via immobilized HER2. It is ideal for bioanalytical assays to measure the level of drug or biosimilar in complex with the HER2 target.

Trastuzumab (trade name Herceptin®) is a drug used in the treatment of HER2 positive breast cancer and other HER2 overexpressing cancers including HER2-positive metastatic cancers of the gastrointestinal tract. Trastuzumab binds to the HER2 (or c-erbB2) proto-oncogene, an EGF receptor-like protein found on 20-30% of breast cancer cells. The binding leads to antibody mediated (complement mediated) killing of the HER2 positive cells.

View a summary of all anti-trastuzumab antibodies

Product Details

Product Form
A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Trastuzumab
Affinity
The monovalent intrinsic affinity of this antibody was measured as KD=31 nM by real time, label-free molecular interaction analysis on immobilized trastuzumab/ErbB2 complex.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Regulatory
For research purposes only

Applications of Trastuzumab antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used as a detection reagent in a direct or an indirect ELISA for trastuzumab captured by immobilized human ErbB2.
Protocol: PK antigen capture ELISA to measure bound drug exclusively

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Hispec Assay Diluent BUF049A 50 ml E IY
LYNX Rapid HRP Antibody Conjugation Kit LNK002P 3 Conjugations For 400µg Antibody CJ
Rat anti DYKDDDDK Tag:HRP MCA4764P 0.1 mg E P WB
Mouse anti Penta Histidine Tag:HRP MCA5995P 125 µl E WB