MHC Class II DR Monomorphic antibody | 37.68

The major histocompatibility complex (MHC) is a cluster of genes some of which are important in the immune response to infections. In sheep, this complex is referred to as the ovine leukocyte antigen (OLA) region. There are 2 major types of MHC class IIa molecules encoded by the OLA which are DR and DQ each composed of an alpha and beta chain.
Product Details
- Target Species
- Sheep
- Species Cross-Reactivity
-
Target Species Cross Reactivity Goat Human Bovine - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Splenocytes from ATL mice.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of MHC Class II DR Monomorphic antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/5 | 1/10 | |
Flow Cytometry | 1/50 | 1/200 | |
Immunohistology - Frozen | |||
Immunoprecipitation |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | F | 0.5 mg |
![]() |
|
Rabbit F(ab')2 anti Mouse IgG:RPE | STAR12A | F | 1 ml |
![]() |
|
Rabbit F(ab')2 anti Mouse IgG:FITC | STAR9B | F | 1 mg |
![]() |
|
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | STAR13B | C E P RE WB | 1 mg |
![]() |
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG2a Negative Control:FITC | MCA929F | F | 100 Tests |
![]() |
|
Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
![]() |
Product Specific References
References for MHC Class II DR Monomorphic antibody
-
Puri, N.K. et al. (1985) Sheep lymphocyte antigens (OLA). II. Major histocompatibility complex class II molecules.
Immunology. 56 (4): 725-33. -
Puri, N.K. et al. (1987) Monoclonal antibodies to sheep MHC class I and class II molecules: biochemical characterization of three class I gene products and four distinct subpopulations of class II molecules.
Vet Immunol Immunopathol. 15 (1-2): 59-86. -
Puri, N.K. & Brandon, M.R. (1987) Sheep MHC class II molecules. II. Identification and characterization of four distinct subsets of sheep MHC class II molecules.
Immunology. 62 (4): 575-80. -
Puri, N.K. et al. (1987) Monoclonal antibodies to sheep MHC class II molecules recognize all HLA-D or subsets of HLA-D region products.
Hum Immunol. 20 (3): 195-207. -
Ballingall, K.T. et al. (1995) Analysis of the fine specificities of sheep major histocompatibility complex class II-specific monoclonal antibodies using mouse L-cell transfectants.
Anim Genet. 26 (2): 79-84. -
Wang, Y. et al. (2017) Characterization of a secreted cystatin of the parasitic nematode Haemonchus contortus and its immune-modulatory effect on goat monocytes.
Parasit Vectors. 10 (1): 425. -
Wang, Y. et al. (2020) Characterization of a rhodanese homologue from Haemonchus contortus and its immune-modulatory effects on goat immune cells in vitro.
Parasit Vectors. 13 (1): 454.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra