MHC Class II DR Monomorphic antibody | 37.68
FITC conjugated Mouse anti Sheep MHC class II antibody, clone 37.68 (MCA2226F) used to evaluate MHC class II expression on goat monocytes by flow cytometry.
Image caption:
rFg-CaBP4-mediated differential expression of major histocompatibility complex (MHC) on the surface of goat monocytes. Monocytes were cultured in the presence of the indicated concentrations of rFg-CaBP4 and control buffer for 24 h. MHC class-I (MHC-I) and MHC-II expressions were measured by flow cytometric analysis and calculated as the percentage of mean fluorescence intensity (MFI). The data are presented as the MFI ± SD from three independent experiments. **P <0.01, ***P <0.001, ****P <0.0001, ns (compared to untreated control)
From: Ehsan M, Hu RS, Hou JL, Elsheikha HM, Li XD, Liang PH, Zhu XQ.
Fasciola gigantica tegumental calcium-binding EF-hand protein 4 exerts immunomodulatory effects on goat monocytes.
Parasit Vectors. 2021 May 22;14(1):276.
doi: 10.1186/s13071-021-04784-5.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Sheep MHC class II antibody, clone 37.68 recognizes a monomorphic epitope on ovine MHC class II DR molecules, constitutively expressed on antigen presenting cells such as dendritic cells, B lymphocytes, monocytes, macrophages, activated T lymphocytes and may be induced on a range of other cell types by interferon gamma.
The major histocompatibility complex (MHC) is a cluster of genes some of which are important in the immune response to infections. In sheep, this complex is referred to as the ovine leukocyte antigen (OLA) region. There are 2 major types of MHC class IIa molecules encoded by the OLA which are DR and DQ each composed of an alpha and beta chain.
The major histocompatibility complex (MHC) is a cluster of genes some of which are important in the immune response to infections. In sheep, this complex is referred to as the ovine leukocyte antigen (OLA) region. There are 2 major types of MHC class IIa molecules encoded by the OLA which are DR and DQ each composed of an alpha and beta chain.
Product Details
- Target Species
- Sheep
- Species Cross-Reactivity
-
Target Species Cross Reactivity Goat Human Bovine - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Splenocytes from ATL mice.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of MHC Class II DR Monomorphic antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/5 | 1/10 | |
| Flow Cytometry | 1/50 | 1/200 | |
| Immunohistology - Frozen | |||
| Immunoprecipitation |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | F | 0.5 mg |
|
|
| Rabbit F(ab')2 anti Mouse IgG:RPE | STAR12A | F | 1 ml |
|
|
| Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | STAR13B | C E P RE WB | 1 mg |
|
|
| Rabbit F(ab')2 anti Mouse IgG:FITC | STAR9B | F | 1 mg |
|
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control:FITC | MCA929F | F | 100 Tests |
|
|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
|
Product Specific References
References for MHC Class II DR Monomorphic antibody
-
Puri, N.K. et al. (1985) Sheep lymphocyte antigens (OLA). II. Major histocompatibility complex class II molecules.
Immunology. 56 (4): 725-33. -
Puri, N.K. et al. (1987) Monoclonal antibodies to sheep MHC class I and class II molecules: biochemical characterization of three class I gene products and four distinct subpopulations of class II molecules.
Vet Immunol Immunopathol. 15 (1-2): 59-86. -
Puri, N.K. & Brandon, M.R. (1987) Sheep MHC class II molecules. II. Identification and characterization of four distinct subsets of sheep MHC class II molecules.
Immunology. 62 (4): 575-80. -
Puri, N.K. et al. (1987) Monoclonal antibodies to sheep MHC class II molecules recognize all HLA-D or subsets of HLA-D region products.
Hum Immunol. 20 (3): 195-207. -
Ballingall, K.T. et al. (1995) Analysis of the fine specificities of sheep major histocompatibility complex class II-specific monoclonal antibodies using mouse L-cell transfectants.
Anim Genet. 26 (2): 79-84. -
Wang, Y. et al. (2017) Characterization of a secreted cystatin of the parasitic nematode Haemonchus contortus and its immune-modulatory effect on goat monocytes.
Parasit Vectors. 10 (1): 425. -
Wang, Y. et al. (2020) Characterization of a rhodanese homologue from Haemonchus contortus and its immune-modulatory effects on goat immune cells in vitro.
Parasit Vectors. 13 (1): 454. -
Ehsan, M. et al. (2021) Fasciola gigantica. tegumental calcium-binding EF-hand protein 4 exerts immunomodulatory effects on goat monocytes.
Parasit Vectors. 14 (1): 276.
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