Mouse anti Sheep Interleukin-6 antibody, clone 4B6 recognizes ovine interleukin-6 (IL-6) and has also been reported to recognise recombinant human IL-6 and bovine IL-6 transfected cells. Mouse anti Sheep Interleukin-6 antibody, clone 4B6 does not cross react with ovine IL-1 beta, IL-8, MCP-1 or TNF alpha.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Recombinant ovine IL-6.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
interleukin-6 receptor binding
growth factor activity
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IL-6 antibody
||5ug/ml (as a coating antibody)
|Flow Cytometry 1
- 1 Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- MCA1659 may also be used in combination with AHP424 in a sandwich ELISA assays for ovine IL-6.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
Product Specific References
References for IL-6 antibody
McWaters, P. et al. (2000) Characterisation of monoclonal antibodies to ovine interleukin-6 and the development of a sensitive capture ELISA.
Vet Immunol Immunopathol. 73 (2): 155-65.
Wang, Z. et al. (2008) Acute hypercapnia improves indices of tissue oxygenation more than dobutamine in septic shock.
Am J Respir Crit Care Med. 177: 178-83.
Su, F. et al (2007) Beneficial effects of ethyl pyruvate in septic shock from peritonitis.
Arch Surg.142: 166-71.
Kabaroff, L. et al. (2006) Changes in ovine maternal temperature, and serum cortisol and interleukin-6 concentrations after challenge with Escherichia coli lipopolysaccharide during pregnancy and early lactation.
J Anim Sci. 84: 2083-8.
Shashikant, B.N. et al. (2005) Dose response to rhCC10-augmented surfactant therapy in a lamb model of infant respiratory distress syndrome: physiological, inflammatory, and kinetic profiles.
J Appl Physiol. 99: 2204-11.
Redondo, E. et al. (2014) Induction of interleukin-8 and interleukin-12 in neonatal ovine lung following experimental inoculation of bovine respiratory syncytial virus.
J Comp Pathol. 150 (4): 434-48.
Karisnan, K .et al. (2015) Interleukin-1 Receptor Antagonist Protects against Lipopolysaccharide Induced Diaphragm Weakness in Preterm Lambs.
PLoS One. 10 (4): e0124390.
Xu, A. et al. (2015) The Ovine Fetal and Placental Inflammatory Response to Umbilical Cord Occlusions With Worsening Acidosis.
Reprod Sci. 22 (11): 1409-20.
Dooley, L.M. et al. (2015) Effect of mesenchymal precursor cells on the systemic inflammatory response and endothelial dysfunction in an ovine model of collagen-induced arthritis.
PLoS One. 10 (5): e0124144.
Herry, C.L. et al. (2016) Temporal Patterns in Sheep Fetal Heart Rate Variability Correlate to Systemic Cytokine Inflammatory Response: A Methodological Exploration of Monitoring Potential Using Complex Signals Bioinformatics.
PLoS One. 11 (4): e0153515.
Ciliberti, M.G. et al. (2017) Peripheral blood mononuclear cell proliferation and cytokine production in sheep as affected by cortisol level and duration of stress.
J Dairy Sci. 100 (1): 750-756.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸
Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra