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CD44 antibody | 25.32

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Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.1 mg loader
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Mouse anti Sheep CD44 antibody, clone 25.32 recognises the ovine CD44 cell surface antigen, a ~95 kDa glycoprotein expressed by most leucocytes and a subpopulation of thymocytes. In immunohistochemical staining, this antibody labels several cell types including medullary thymocytes, kupffer cells, kidney tubular epithelial cells and gut smooth muscle cells. The expression of CD44 is upregulated upon cell activation.

Mouse anti Sheep CD44 antibody, clone 25.32 is a valuable ragent for the isolation and characterization of ovine mesenchymal stem cells along with CD29 and CD166 which are also expressed on this cell type, in contrast to hematopoietic sell markers including CD45 which is negative on this mesenchymal stem sell population (Sanjurjo-Rodríguez et al. 2017).

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Ovine efferent lymphatic duct lymphocytes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 1 x 106 cells in 100μl

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Source Reference

  1. Mackay, C.R. et al. (1988) Characterization of a 95,000 molecule on sheep leucocytes homologous to murine Pgp-1 and human CD44.
    Immunology. 65 (1): 93-9.

References for CD44 antibody

  1. Aleksandersen, M. et al. (1990) Distribution of lymphocyte subsets in the large intestinal lymphoid follicles of lambs.
    Immunology. 70 (3): 391-7.
  2. Witherden, D.A. et al. (1995) Antigen-independent maturation of CD2, CD11a/CD18, CD44, and CD58 expression on thymic emigrants in fetal and postnatal sheep.
    Dev Immunol. 4:199-209
  3. Stevenson, L.M. et al. (2001) Expression of cell surface adhesion molecules by peripheral blood eosinophils during Trichostrongylus colubriformis infection in sheep.
    Immunol Cell Biol. 79 (3): 240-4.
  4. Perry, K. et al. (2010) Hyaluronan (HA) content, the ratio of HA fragments and the expression of CD44 in the ovine cervix vary with the stage of the oestrous cycle.
    Reproduction. 140:133-41.
  5. Sanjurjo-Rodríguez, C. et al. (2017) Ovine Mesenchymal Stromal Cells: Morphologic, Phenotypic and Functional Characterization for Osteochondral Tissue Engineering.
    PLoS One. 12 (1): e0171231.
  6. Wooldridge, A.L. et al. (2019) Maternal allergic asthma during pregnancy alters fetal lung and immune development in sheep: potential mechanisms for programming asthma and allergy.
    J Physiol. 597 (16): 4251-62.
  7. López-Fernández, A. et al. (2020) Effect of Allogeneic Cell-Based Tissue-Engineered Treatments in a Sheep Osteonecrosis Model.
    Tissue Eng Part A. 26 (17-18): 993-1004.
  8. Savy, V. et al. (2021) Effect of Embryo Aggregation on In Vitro Development of Adipose-Derived Mesenchymal Stem Cell-Derived Bovine Clones.
    Cell Reprogram. 23 (5): 277-289.
  9. View The Latest Product References
  10. Zhang, P. et al. (2021) Differences in the biological properties of mesenchymal stromal cells from traumatic temporomandibular joint fibrous and bony ankylosis: a comparative study
    Animal Cells and Systems. : 1-16.
  11. Castillo, M.G. et al. (2023) Promoting early neovascularization by allotransplanted adipose-derived Muse cells in an ovine model of acute myocardial infarction.
    PLoS One. 18 (1): e0277442.



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