CD44 antibody | 25.32

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Mouse anti Sheep CD44:FITC

Mouse anti Sheep CD44

Product Type
Monoclonal Antibody
Clone
25.32
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA2219F
Datasheet
F
MSDS
0.1 mg loader

MCA2219GA
Datasheet
C F IP
MSDS
0.1 mg loader

Mouse anti Sheep CD44 antibody, clone 25.32 recognises the ovine CD44 cell surface antigen, a ~95 kDa glycoprotein expressed by most leucocytes and a subpopulation of thymocytes. In immunohistochemical staining, this antibody labels several cell types including medullary thymocytes, kupffer cells, kidney tubular epithelial cells and gut smooth muscle cells. The expression of CD44 is upregulated upon cell activation.

Mouse anti Sheep CD44 antibody, clone 25.32 is a valuable ragent for the isolation and characterization of ovine mesenchymal stem cells along with CD29 and CD166 which are also expressed on this cell type, in contrast to hematopoietic sell markers including CD45 which is negative on this mesenchymal stem sell population (Sanjurjo-Rodríguez et al. 2017).

Product Details

Target Species
Sheep
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
Goat
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Ovine efferent lymphatic duct lymphocytes
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.
Guarantee
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of CD44 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry 1/50 1/100
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
Mouse IgG1 Negative Control MCA928 F 100 Tests loader

Product Specific References

References for CD44 antibody

  1. Mackay, C.R. et al. (1988) Characterization of a 95,000 molecule on sheep leucocytes homologous to murine Pgp-1 and human CD44.
    Immunology. 65 (1): 93-9.
  2. Aleksandersen, M. et al. (1990) Distribution of lymphocyte subsets in the large intestinal lymphoid follicles of lambs.
    Immunology. 70 (3): 391-7.
  3. Perry, K. et al. (2010) Hyaluronan (HA) content, the ratio of HA fragments and the expression of CD44 in the ovine cervix vary with the stage of the oestrous cycle.
    Reproduction. 140:133-41.
  4. Stevenson, L.M. et al. (2001) Expression of cell surface adhesion molecules by peripheral blood eosinophils during Trichostrongylus colubriformis infection in sheep.
    Immunol Cell Biol. 79 (3): 240-4.
  5. Witherden, D.A. et al. (1995) Antigen-independent maturation of CD2, CD11a/CD18, CD44, and CD58 expression on thymic emigrants in fetal and postnatal sheep.
    Dev Immunol. 4:199-209
  6. Sanjurjo-Rodríguez, C. et al. (2017) Ovine Mesenchymal Stromal Cells: Morphologic, Phenotypic and Functional Characterization for Osteochondral Tissue Engineering.
    PLoS One. 12 (1): e0171231.
  7. Wooldridge, A.L. et al. (2019) Maternal allergic asthma during pregnancy alters fetal lung and immune development in sheep: potential mechanisms for programming asthma and allergy.
    J Physiol. 597 (16): 4251-62.