CD44 antibody | 25.32
FITC conjugated Mouse anti Ovine CD44 antibody, clone 25.32 (MCA2219F) used for the evaluation of CD44 expression on bone marrow derived ovine mesenchymal stem cells by flow cytometry.
Image caption:
Morphologic and phenotypic characterization of oBMSCs.
(A) Image of ovine bone marrow mesenchymal stromal cells at passage 5 and 8 isolated from iliac crest aspirates. (B) Image of human bone marrow mesenchymal stromal cells (hBMSCs) at passage 10 isolated from bone marrow aspirates, as previously described [Sanjurjo-Rodríguez 2014]. Both images show the typical spindle-shaped fibroblast-like morphology characteristic of the MSC. Original magnification x100. (C) Phenotypic characterization by flow cytometry of a representative population of oBMSCs, for markers characteristic of MSCs and hematopoietic cells that react with sheep. The purple line signifies the specific antibody, while the orange line represents the isotype control. Plots correspond to a representative experiment (D) Bar graph representing the percentage average values (mean ± standard error) of positivity of the markers analyzed from 3 samples at different passages (at 3rd, 4th and 8th passages); markers characteristic of MSCs (CD29, CD44, CD166), embryonic cells (SSEA-4), and hematopoietic cells (CD45).
From: Sanjurjo-Rodríguez C, Castro-Viñuelas R, Hermida-Gómez T, Fernández-Vázquez T, Fuentes-Boquete IM, de Toro-Santos FJ, et al. (2017)
Ovine Mesenchymal Stromal Cells: Morphologic, Phenotypic and Functional Characterization for Osteochondral Tissue Engineering.
PLoS ONE 12(1): e0171231.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
FITC conjugated Mouse anti Sheep CD44 antibody, clone 25.32 (MCA2220F) used to evaluate CD44 expression on sheep cells by flow cytometry
Image caption:
Flow cytometry expression analysis.
Labeling of A: AT-Muse cells and B: ASCs with antibodies anti- SSEA3, CD45, CD44, CD166 and controls.
From: Castillo MG, Peralta TM, Locatelli P, Velazquez C, Herrero Y, Crottogini AJ, et al. (2023)
Promoting early neovascularization by allotransplanted adipose-derived Muse cells in an ovine model of acute myocardial infarction.
PLoS ONE 18(1): e0277442.
doi:10.1371/journal.pone.0277442
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Sheep CD44
- Product Type
- Monoclonal Antibody
- Clone
- 25.32
- Isotype
- IgG1
- Specificity
- CD44
Antibody Quality and Performance Guarantee
| Mouse anti Sheep CD44 antibody, clone 25.32 recognises the ovine CD44 cell surface antigen, a ~95 kDa glycoprotein expressed by most leucocytes and a subpopulation of thymocytes. In immunohistochemical staining, this antibody labels several cell types including medullary thymocytes, kupffer cells, kidney tubular epithelial cells and gut smooth muscle cells. The expression of CD44 is upregulated upon cell activation. Mouse anti Sheep CD44 antibody, clone 25.32 is a valuable ragent for the isolation and characterization of ovine mesenchymal stem cells along with CD29 and CD166 which are also expressed on this cell type, in contrast to hematopoietic sell markers including CD45 which is negative on this mesenchymal stem sell population (Sanjurjo-Rodríguez et al. 2017). |
- Target Species
- Sheep
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Goat Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Ovine efferent lymphatic duct lymphocytes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1 x 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | F | 0.5 mg |
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| Description | Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | ||||||
| Rabbit F(ab')2 anti Mouse IgG:RPE | STAR12A | F | 1 ml |
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| Description | Rabbit F(ab')2 anti Mouse IgG:RPE | ||||||
| Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | STAR13B | C E P RE WB | 1 mg |
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| Description | Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | ||||||
| Rabbit F(ab')2 anti Mouse IgG:FITC | STAR9B | F | 1 mg |
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| Description | Rabbit F(ab')2 anti Mouse IgG:FITC | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
-
Mackay, C.R. et al. (1988) Characterization of a 95,000 molecule on sheep leucocytes homologous to murine Pgp-1 and human CD44.
Immunology. 65 (1): 93-9.
References for CD44 antibody
-
Aleksandersen, M. et al. (1990) Distribution of lymphocyte subsets in the large intestinal lymphoid follicles of lambs.
Immunology. 70 (3): 391-7. -
Witherden, D.A. et al. (1995) Antigen-independent maturation of CD2, CD11a/CD18, CD44, and CD58 expression on thymic emigrants in fetal and postnatal sheep.
Dev Immunol. 4:199-209 -
Stevenson, L.M. et al. (2001) Expression of cell surface adhesion molecules by peripheral blood eosinophils during Trichostrongylus colubriformis infection in sheep.
Immunol Cell Biol. 79 (3): 240-4. -
Perry, K. et al. (2010) Hyaluronan (HA) content, the ratio of HA fragments and the expression of CD44 in the ovine cervix vary with the stage of the oestrous cycle.
Reproduction. 140:133-41. -
Sanjurjo-Rodríguez, C. et al. (2017) Ovine Mesenchymal Stromal Cells: Morphologic, Phenotypic and Functional Characterization for Osteochondral Tissue Engineering.
PLoS One. 12 (1): e0171231. -
Wooldridge, A.L. et al. (2019) Maternal allergic asthma during pregnancy alters fetal lung and immune development in sheep: potential mechanisms for programming asthma and allergy.
J Physiol. 597 (16): 4251-62. -
López-Fernández, A. et al. (2020) Effect of Allogeneic Cell-Based Tissue-Engineered Treatments in a Sheep Osteonecrosis Model.
Tissue Eng Part A. 26 (17-18): 993-1004. -
Savy, V. et al. (2021) Effect of Embryo Aggregation on In Vitro Development of Adipose-Derived Mesenchymal Stem Cell-Derived Bovine Clones.
Cell Reprogram. 23 (5): 277-289.
View The Latest Product References
-
Zhang, P. et al. (2021) Differences in the biological properties of mesenchymal stromal cells from traumatic temporomandibular joint fibrous and bony ankylosis: a comparative study
Animal Cells and Systems. : 1-16. -
Castillo, M.G. et al. (2023) Promoting early neovascularization by allotransplanted adipose-derived Muse cells in an ovine model of acute myocardial infarction.
PLoS One. 18 (1): e0277442.
- Synonyms
- H-CAM
- PGP-1
MCA2219GA
0215
166462
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