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Risankizumab antibody | AbD58231

Human anti Risankizumab

Product Type
Monoclonal Antibody
Clone
AbD58231
Isotype
Fab antibody
Specificity
Risankizumab

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Human Anti-Risankizumab Antibody, clone AbD58231 is a paratope specific, inhibitory, anti-idiotypic antibody (Type 1) that specifically recognizes the free human monoclonal antibody drug risankizumab, and its biosimilar products. It does not recognize the drug target, human Interleukin 23 (IL-23), nor risankizumab in complex with IL-23. This antibody can be used to measure free risankizumab levels in serum from patients.

Clone AbD58231 is available as a monovalent Fab antibody incorporating a SpyTag at the C-terminus end of the antibody heavy chain for conversion by the end user into alternative formats using any of the SpyCatchers available in our catalog under product code TZA059. It is also available as a bivalent Fab antibody already conjugated to HRP via coupling to the HRP conjugated BiSpyCatcher2 (TZC002P) for use in direct ELISA under product code TZA059P.

A pair of anti-risankizumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug; clone AbD58231 is recommended as a detection antibody, paired with Human Anti-Risankizumab Antibody, clone AbD59012ad (TZA058) as the capture reagent.

Risankizumab (trade name Skyrizi) is a humanized monoclonal antibody of the IgG1/kappa isotype that suppresses the inflammatory effects of interleukin-23 by selectively targeting its unique p19 subunit, subsequently inhibiting IL-23 binding to its receptor and the signaling responsible for the release of pro-inflammatory cytokines and chemokines. Risankizumab is indicated as an interleukin-23 antagonist in the treatment of plaque psoriasis, psoriatic arthritis and Crohn's disease in adults.

Product Form
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL phage display library and expressed in a proprietary E. coli. strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography
Source
E.coli
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.0095% MIT
Immunogen
Risankizumab
Affinity
The monovalent intrinsic affinity of Human anti-Risankizumab Antibody, clone AbD58231ad was measured as KD = 0.2 nM by real time, label-free molecular interaction analysis on immobilized risankizumab.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Skyrizi is a registered trademark of AbbVie Biotechnology Ltd.
Licensed Use
For in vitro. research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
ELISA
Clone AbD58231ad can be used in indirect ELISA to detect risankizumab. It can also be used as a detection antibody to develop a pharmacokinetic (PK) bridging ELISA for risankizumab, together with TZA058 as the capture reagent. Note that both TZA058 (capture) and TZA059 (detection) contain the same peptide tags. Therefore, using anti-tag antibodies for detecting TZA059 is not possible.

Synonyms
Skyrizi
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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