Risankizumab antibody | AbD59012ad
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using Human Anti-Risankizumab Antibody, clone AbD59012ad (TZA058) at a concentration of 2 μg/ml followed by HRP conjugated Anti-DYKDDDDK Tag Antibody in HISPEC Assay Diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate.
A microtiter plate was coated overnight with risankizumab, human IgG1 kappa control, human IgG1 lambda control or recombinant human IL-23 at a concentration of 5 µg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using Human Anti-Risankizumab, clone AbD59012ad (TZA058) titrated to the given concentrations followed by an HRP conjugated Anti-His Tag Antibody in HISPEC Assay Diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate. Data for risankizumab are shown as the mean of three measurements.
A microtiter plate was coated overnight with Human Anti-Risankizumab Antibody, clone AbD59012ad (TZA058) at a concentration of 1 µg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of risankizumab. Detection was performed using HRP-conjugated Human Anti-Risankizumab Antibody, clone AbD58231pap (TZA059P) at a concentration of 0.5 µg/mL in HISPEC Assay Diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate. Data are shown as the mean of three measurements.
A microtiter plate was coated overnight with recombinant human IL-23 at a concentration of 1 µg/ml. After washing and blocking with PBST + 5% BSA, a pre-incubated mixture of risankizumab (0.15 µg/ml) plus increasing concentrations of Human Anti-Risankizumab Antibody, clone AbD59012ad (TZA058). Free risankizumab, still capable of binding to the IL-23 coated plate, was detected using HRP conjugated Mouse Anti-Human IgG (Fc) CH2 Domain Antibody (MCA647P) in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu fluorogenic peroxidase substrate. Data are shown as the mean of three measurements.
Filter by Application:
E ResetHuman anti Risankizumab
- Product Type
- Monoclonal Antibody
- Clone
- AbD59012ad
- Isotype
- Fab antibody
- Specificity
- Risankizumab
Antibody Quality and Performance Guarantee
TZA058
| Human Anti-Risankizumab Antibody, clone AbD59012ad is a paratope specific, inhibitory, anti-idiotypic antibody (Type 1) that specifically recognizes the free human monoclonal antibody drug risankizumab, and its biosimilar products. It does not recognize the drug target, human Interleukin 23 (IL-23), nor risankizumab in complex with IL-23. This antibody can be used to measure free risankizumab levels in serum from patients. A pair of anti-risankizumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug; clone AbD59012ad is recommended as a capture antibody, paired with Human Anti-Risankizumab Antibody, clone AbD58231 (TZA059) as the detection antibody. TZA058 a monovalent Fab antibody incorporating a SpyTag at the C-terminus end of the antibody heavy chain for conversion by the end user into alternative formats using any of the SpyCatchers available in our catalog. It is also available in the full length recombinant human IgG1 format under product code HCA414. Risankizumab (trade name Skyrizi) is a humanized monoclonal antibody of the IgG1/kappa isotype that suppresses the inflammatory effects of interleukin-23 by selectively targeting its unique p19 subunit, subsequently inhibiting IL-23 binding to its receptor and the signaling responsible for the release of pro-inflammatory cytokines and chemokines. Risankizumab is indicated as an interleukin-23 antagonist in the treatment of plaque psoriasis, psoriatic arthritis and Crohn's disease in adults. |
- Product Form
- A monovalent human recombinant Fab (kappa light chain) selected from the HuCAL phage display library and expressed in a proprietary E. coli. strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.0095% MIT
- Immunogen
- Risankizumab
- Affinity
- The monovalent intrinsic affinity of Human Anti-Risankizumab Antibody, clone AbD59012ad was measured as KD = 0.1 nM by real time, label-free molecular interaction analysis on immobilized risankizumab.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Skyrizi is a registered trademark of AbbVie Biotechnology Ltd. - Licensed Use
- For in vitro. research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
- Clone AbD59012ad can be used in indirect ELISA to detect risankizumab. It can also be used as the capture antibody to develop a pharmacokinetic (PK) bridging ELISA for risankizumab, together with TZA059 or TZA059P as the detection reagent.
- Synonyms
- Skyrizi
View more products with Risankizumab specificity
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