PCNA antibody | PC10

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry 1			1/100
Immunohistology - Frozen
Immunohistology - Paraffin		1/50	1/100
Immunoprecipitation
Western Blotting			1/1000

1. ¹ Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry

Use 10μl of the suggested working dilution to label 1x10⁶ cells in 100μl

Histology Positive Control Tissue

Tonsil

References for PCNA antibody

1. Waseem, N.H. & Lane, D.P. (1990) Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form.
   J Cell Sci. 96 ( Pt 1): 121-9.
2. Landberg, G. et al. (1990) Flow cytometric multiparameter analysis of proliferating cell nuclear antigen/cyclin and Ki-67 antigen: a new view of the cell cycle.
   Exp Cell Res. 187 (1): 111-8.
3. Wilson, G.D. et al. (1992) Flow cytometric characterisation of proliferating cell nuclear antigen using the monoclonal antibody PC10.
   Eur J Cancer. 28A (12): 2010-7.
4. Jenkins, H. et al. (1993) Nuclei that lack a lamina accumulate karyophilic proteins and assemble a nuclear matrix.
   J Cell Sci. 106: 275-85.
5. Prosperi, E. et al. (1993) Proliferating cell nuclear antigen complex formation induced by ultraviolet irradiation in human quiescent fibroblasts as detected by immunostaining and flow cytometry.
   Exp Cell Res. 205 (2): 320-5.
6. Elsässer, H.P. et al. (1994) Growth of rat pancreatic acinar cells quantitated with a monoclonal antibody against the proliferating cell nuclear antigen.
   Cell Tissue Res. 276 (3): 603-9.
7. Buggins, A.G. et al. (2001) Microenvironment produced by acute myeloid leukemia cells prevents T cell activation and proliferation by inhibition of NF-kappaB, c-Myc, and pRb pathways.
   J Immunol. 167: 6021-30.
8. Fenton, M. et al. (2001) Cellular senescence after single and repeated balloon catheter denudations of rabbit carotid arteries.
   Arterioscler Thromb Vasc Biol. 21: 220-6.
View The Latest Product References
9. Harris, L. et al. (2006) Characterisation of cell types in abalone (Haliotis spp.) tissues using immunohistochemical techniques
   Aquaculture 261: 1413-21
10. Park, J.H. et al. (2008) Gastric lesions and immune responses caused by long-term infection with Helicobacter heilmannii in C57BL/6 mice.
    J Comp Pathol. 139: 208-17.
11. Franz-Odendaal, T.A. (2008) Toward understanding the development of scleral ossicles in the chicken, Gallus gallus.
    Dev Dyn. 237: 3240-51.
12. Kapitonova, M.Y. et al. (2010) Immunohistochemical characteristics of the hypophysis in normal conditions and chronic stress.
    Neurosci Behav Physiol. 40: 97-102.
13. Hashimoto, Y. et al. (2010) Rad51 protects nascent DNA from Mre11-dependent degradation and promotes continuous DNA synthesis.
    Nat Struct Mol Biol. 17: 1305-11.
14. Izhak, L. et al. (2012) Dissecting the autocrine and paracrine roles of the CCR2-CCL2 axis in tumor survival and angiogenesis.
    PLoS One. 7: e28305.
15. Khlebnikov, V.V. et al. (2015) Developmental Characteristics of the Hypothalamo-Hypophyseal-Adrenal System in Chronic Heterotypical Stress
    Neuroscience and Behavioral Physiology. 46 (1): 100-5.
16. Guzera, M. et al. (2016) In Vitro Influence of Mycophenolic Acid on Selected Parameters of Stimulated Peripheral Canine Lymphocytes.
    PLoS One. 11 (5): e0154429.
17. Di-poï, N. & Milinkovitch, M.C. (2016) The anatomical placode in reptile scale morphogenesis indicates shared ancestry among skin appendages in amniotes.
    Sci Adv. 2 (6): e1600708.
18. Nakatsuka, M. & Kumabe, S (2018) Histological Alterations from Condyle Repositioning with Functional Appliances in Rats.
    J Clin Pediatr Dent. 42 (5): 391-7.
19. Falbo, L. et al. (2020) SSRP1-mediated histone H1 eviction promotes replication origin assembly and accelerated development.
    Nat Commun. 11 (1): 1345.
20. Kurdi, A. et al. (2019) Everolimus depletes plaque macrophages, abolishes intraplaque neovascularization and improves survival in mice with advanced atherosclerosis.
    Vascul Pharmacol. 113: 70-6.

Immunohistology - Paraffin

Synonyms

Proliferating Cell Nuclear Antigen

RRID

AB_324955

UniProt

P04961

Entrez Gene

Pcna

GO Terms

GO:0006260 DNA replication

GO:0043626 PCNA complex

GO:0006275 regulation of DNA replication

GO:0007507 heart development

GO:0046686 response to cadmium ion

GO:0019985 translesion synthesis

GO:0030337 DNA polymerase processivity factor activity

GO:0032077 positive regulation of deoxyribonuclease activity

GO:0033993 response to lipid

View More GO Terms

GO:0070557 PCNA-p21 complex

MCA1558

1114 160557

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