Mouse IgG2a Negative Control antibody

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Mouse IgG2a Negative Control Antibody - Human thumbnail image 2

Mouse IgG2a Negative Control Antibody - Human thumbnail image 3

Mouse IgG2a Negative Control Antibody - Human thumbnail image 4

Mouse IgG2a Negative Control Antibody - Human thumbnail image 5

Mouse IgG2a Negative Control Antibody - Human thumbnail image 6

Mouse IgG2a Negative Control Antibody - Human thumbnail image 7

Mouse IgG2a Negative Control Antibody - Human gallery image 1 — Figure A. FITC conjugated mouse anti rat CD3 (MCA772F) and RPE conjugated mouse IgG2a isotype control (**MCA1210PE**). Figure B. FITC conjugated mouse anti rat CD3 (MCA772F) and RPE conjugated mouse anti rat CD2 (MCA154PE). All experiments performed on red cell lysed rat blood gated on mononuclear cells.

Mouse IgG2a Negative Control Antibody - Human gallery image 2 — Figure A. RPE conjugated mouse anti rat CD45 (MCA43PE) and FITC conjugated mouse IgG2a isotype control (**MCA1210F**). Figure B. RPE conjugated mouse anti rat CD45 (MCA43PE) and FITC conjugated mouse anti rat CD26 (MCA924F). All experiments performed on red cell lysed rat blood gated on mononuclear cells.

Mouse IgG2a Negative Control Antibody - Human gallery image 3 — Figure A. A647 conjugated mouse anti rat CD4 (MCA153A647) and RPE conjugated mouse IgG2a isotype control (**MCA1210PE**). Figure B. A647 conjugated mouse anti rat CD4 (MCA153A647) and RPE conjugated mouse anti rat CD44 (MCA643PE). All experiments performed on red cell lysed rat blood gated on mononuclear cells.

Mouse IgG2a Negative Control Antibody - Human gallery image 4 — Figure A. RPE conjugated mouse anti rat CD44 (MCA643PE) and A647 conjugated mouse IgG2a isotype control (**MCA1210A647**). Figure B. RPE conjugated mouse anti rat CD44 (MCA643PE) and RPE conjugated mouse anti rat CD4 (MCA153A647). All experiments performed on red cell lysed rat blood gated on mononuclear cells.

Mouse IgG2a Negative Control Antibody - Human gallery image 5 — Figure A. FITC conjugated mouse anti rat CD45 (MCA43FB) and RPE conjugated mouse IgG2a isotype control (**MCA1210PE**). Figure B. FITC conjugated mouse anti rat CD45 (MCA43FB) and RPE conjugated mouse anti rat CD49d (MCA2872PE). All experiments performed on red cell lysed rat blood gated on mononuclear cells.

Mouse IgG2a Negative Control Antibody - Human gallery image 6 — Figure A. PE conjugated mouse anti rat CD106 (MCA4633PE) and FITC conjugated mouse IgG2a isotype control (**MCA1210F**). Figure B. PE conjugated mouse anti rat CD106 (MCA4633PE) and FITC conjugated mouse anti rat CD11b (MCA619F). All experiments performed on red cell lysed rat bone marrow gated on mononuclear cells.

Mouse IgG2a Negative Control Antibody - Human gallery image 7 — Figure A. PE conjugated mouse anti rat CD172a (MCA620PE) and FITC conjugated mouse IgG2a isotype control (**MCA1210F**). Figure B. PE conjugated mouse anti rat CD172a (MCA620PE) and FITC conjugated mouse anti rat CD11c (MCA1441F). All experiments performed on rat peritoneal macrophages.

Mouse IgG2a Negative Control:Alexa Fluor® 488

Mouse IgG2a isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a human surface antigen.

Mouse IgG2a Negative Control:Alexa Fluor® 647

Mouse IgG2a isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a human surface antigen.

Mouse IgG2a Negative Control:Preservative Free

Mouse IgG2a isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a human surface antigen.

Mouse IgG2a Negative Control:Biotin

Mouse IgG2a isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a human surface antigen.

Mouse IgG2a Negative Control:FITC

Mouse IgG2a isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a human surface antigen.

Mouse IgG2a Negative Control:Low Endotoxin

Mouse IgG2a isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a human surface antigen.

Mouse IgG2a Negative Control:Pacific Blue®

Mouse IgG2a isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a human surface antigen.

Mouse IgG2a Negative Control

Mouse IgG2a isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a human surface antigen.

Mouse IgG2a Negative Control:RPE

Mouse IgG2a isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a human surface antigen.

Mouse IgG2a Negative Control:RPE-Alexa Fluor® 647

Mouse IgG2a isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a human surface antigen.

Mouse IgG2a Negative Control:Alexa Fluor® 700

Mouse IgG2a isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a human surface antigen.

Product Type: Negative/Isotype Control
Isotype: IgG2a

Product Code	Applications	Pack Size
MCA1210A488	F	100 Tests/1ml
MCA1210A647	F	100 Tests/1ml
MCA1210XZ	F	1 mg
MCA1210B	F	0.1 mg
MCA1210F	F	0.1 mg
MCA1210EL	F	0.5 mg
MCA1210PB	F	100 Tests/1ml
MCA1210	F	100 Tests
MCA1210PE	F	100 Tests
MCA1210P647	F	100 Tests/1ml
MCA1210A700	F	100 Tests/1ml

Summary
References (1)

Mouse IgG2a Negative Control is suitable for use as a matched isotype negative control reagent to assess the level of non-specific binding of mouse IgG2a monoclonal antibodies to rat cells. This antibody has been tested and found to be negative on the following rat cells – peripheral blood leucocytes, thymocytes, splenocytes and macrophages.

Mouse IgG2a Negative Control (MCA1210) recognizes the human CD45 antigen and therefore human leucocytes may be used as a positive control for this product.

THIS PRODUCT IS NOT SUITABLE FOR USE AS A NEGATIVE CONTROL ON HUMAN TISSUES.

Product Details

Target Species

Negative Control

Product Form

Purified IgG conjugated to Alexa Fluor® 488 - liquid

Product Form

Purified IgG conjugated to Alexa Fluor® 647 - liquid

Product Form

Purified IgG - liquid

Product Form

Purified IgG conjugated to Biotin - liquid

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Product Form

Purified IgG - liquid

Product Form

Purified IgG conjugated to Pacific Blue® - liquid

Product Form

Purified IgG - liquid

Product Form

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized

Product Form

Purified IgG conjugated to R. Phycoerythrin Alexa Fluor®750 - lyophilized

Product Form

Purified IgG conjugated to Alexa Fluor® 700 - liquid

Reconstitution

Reconstitute with 1 ml distilled water

Reconstitution

Reconstitute with 1.0 ml distilled water

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Preparation

Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Preparation

Purified IgG prepared by ion exchange chromatography

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Preparation

Purified IgG prepared by ion exchange chromatography

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Preparation

Purified IgG prepared by ion exchange chromatography

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

None present

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

None present

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin
5%	Sucrose

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin
5%	Sucrose

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Carrier Free

Yes

Carrier Free

Yes

Immunogen

Human T lymphocytes.

Approx. Protein Concentrations

IgG concentration 0.05 mg/ml

Approx. Protein Concentrations

IgG concentration 0.05 mg/ml

Approx. Protein Concentrations

IgG concentration 1 mg/ml

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Approx. Protein Concentrations

IgG concentration 0.1mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Approx. Protein Concentrations

IgG concentration 0.05 mg/ml

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Approx. Protein Concentrations

IgG concentration 0.05 mg/ml

Fusion Partners

Spleen cells from immunized BALB/c mice were fused with cells of the NSI mouse myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at -20^oC only.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at -20^oC only.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Prior to reconstitution store at +4^oC. Following reconstitution store at +4^oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Prior to reconstitution store at +4^oC. Following reconstitution store at +4^oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 12 months from date of reconstitution.
Guarantee: 6 months from date of reconstitution
Guarantee: 18 months from date of despatch.

More Information

Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements: This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory: For research purposes only

Applications of Mouse IgG2a Negative Control antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry			*
Flow Cytometry			*
Flow Cytometry			*
Flow Cytometry			*
Flow Cytometry			*
Flow Cytometry			*
Flow Cytometry			*
Flow Cytometry			*
Flow Cytometry			*
Flow Cytometry			*
Flow Cytometry			*

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.

* It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.

*It is suggested that the user adjusts the concentration of this reagent to be the same as the test reagent.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.

*It is recommended that the user dilutes the antibody in their own system to a concentration equivalent to their test antibody.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *It is recommended that the user dilutes the antibody to a concentration equivalent to their test reagent.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

Product Specific References

References for Mouse IgG2a Negative Control antibody

Fijak, M. et al. (2015) Influence of Testosterone on Inflammatory Response in Testicular Cells and Expression of Transcription Factor Foxp3 in T Cells.
Am J Reprod Immunol. 74 (1): 12-25.

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