Mouse IgG2a Negative Control antibody

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Mouse IgG2a Negative Control Antibody - Human thumbnail image 2

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Mouse IgG2a Negative Control Antibody - Human gallery image 1 — Figure A. FITC conjugated mouse anti rat CD3 (MCA772F) and RPE conjugated mouse IgG2a isotype control (**MCA1210PE**). Figure B. FITC conjugated mouse anti rat CD3 (MCA772F) and RPE conjugated mouse anti rat CD2 (MCA154PE). All experiments performed on red cell lysed rat blood gated on mononuclear cells.

Mouse IgG2a Negative Control Antibody - Human gallery image 2 — Figure A. A647 conjugated mouse anti rat CD4 (MCA153A647) and RPE conjugated mouse IgG2a isotype control (**MCA1210PE**). Figure B. A647 conjugated mouse anti rat CD4 (MCA153A647) and RPE conjugated mouse anti rat CD44 (MCA643PE). All experiments performed on red cell lysed rat blood gated on mononuclear cells.

Mouse IgG2a Negative Control Antibody - Human gallery image 3 — Figure A. RPE conjugated mouse anti rat CD44 (MCA643PE) and A647 conjugated mouse IgG2a isotype control (**MCA1210A647**). Figure B. RPE conjugated mouse anti rat CD44 (MCA643PE) and RPE conjugated mouse anti rat CD4 (MCA153A647). All experiments performed on red cell lysed rat blood gated on mononuclear cells.

Mouse IgG2a Negative Control Antibody - Human gallery image 4 — Figure A. PE conjugated mouse anti rat CD106 (MCA4633PE) and FITC conjugated mouse IgG2a isotype control (**MCA1210F**). Figure B. PE conjugated mouse anti rat CD106 (MCA4633PE) and FITC conjugated mouse anti rat CD11b (MCA619F). All experiments performed on red cell lysed rat bone marrow gated on mononuclear cells.

Mouse IgG2a Negative Control Antibody - Human gallery image 5 — Figure A. PE conjugated mouse anti rat CD172a (MCA620PE) and FITC conjugated mouse IgG2a isotype control (**MCA1210F**). Figure B. PE conjugated mouse anti rat CD172a (MCA620PE) and FITC conjugated mouse anti rat CD11c (MCA1441F). All experiments performed on rat peritoneal macrophages.

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Mouse IgG2a Negative Control:FITC

Product Type: Negative/Isotype Control
Isotype: IgG2a
Specificity: Mouse IgG2a Negative Control

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References (1)
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Batch/Lot Specific Datasheets

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MCA1210F Datasheet Datasheet SDS SDS	F	0.1 mg		Log in	Get Quote
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Mouse IgG2a Negative Control is suitable for use as a matched isotype negative control reagent to assess the level of non-specific binding of mouse IgG2a monoclonal antibodies to rat cells. This antibody has been tested and found to be negative on the following rat cells – peripheral blood leucocytes, thymocytes, splenocytes and macrophages.

Mouse IgG2a Negative Control (MCA1210) recognizes the human CD45 antigen and therefore human leucocytes may be used as a positive control for this product.

THIS PRODUCT IS NOT SUITABLE FOR USE AS A NEGATIVE CONTROL ON HUMAN TISSUES.

Target Species: Negative Control
Product Form: Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% sodium azide (NaN₃)
1% bovine serum albumin
Immunogen: Human T lymphocytes.
Approx. Protein Concentrations: IgG concentration 0.1mg/ml
Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the NSI mouse myeloma cell line.
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

FITC 490 525
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry			*

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.

* It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody.

Flow Cytometry: Use 10μl of the suggested working dilution to label 10⁶ cells in 100μl

References for Mouse IgG2a Negative Control antibody

Fijak, M. et al. (2015) Influence of Testosterone on Inflammatory Response in Testicular Cells and Expression of Transcription Factor Foxp3 in T Cells.
Am J Reprod Immunol. 74 (1): 12-25.