Mouse IgG2a Negative Control antibody
Mouse IgG2a Negative Control:Alexa Fluor® 488
Mouse IgG2a isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a human surface antigen.
Mouse IgG2a Negative Control:Alexa Fluor® 647
Mouse IgG2a isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a human surface antigen.
Mouse IgG2a Negative Control:Preservative Free
Mouse IgG2a isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a human surface antigen.
Mouse IgG2a Negative Control:Biotin
Mouse IgG2a isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a human surface antigen.
Mouse IgG2a Negative Control:FITC
Mouse IgG2a isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a human surface antigen.
Mouse IgG2a Negative Control:Low Endotoxin
Mouse IgG2a isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a human surface antigen.
Mouse IgG2a Negative Control:Pacific Blue®
Mouse IgG2a isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a human surface antigen.
Mouse IgG2a Negative Control
Mouse IgG2a isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a human surface antigen.
Mouse IgG2a Negative Control:RPE
Mouse IgG2a isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a human surface antigen.
Mouse IgG2a Negative Control:RPE-Alexa Fluor® 647
Mouse IgG2a isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a human surface antigen.
Mouse IgG2a Negative Control:Alexa Fluor® 700
Mouse IgG2a isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a human surface antigen.
- Product Type
- Negative/Isotype Control
- Isotype
- IgG2a
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA1210A488 | F | 100 Tests/1ml |
|
|
| MCA1210A647 | F | 100 Tests/1ml |
|
|
| MCA1210XZ | F | 1 mg |
|
|
| MCA1210B | F | 0.1 mg |
|
|
| MCA1210F | F | 0.1 mg |
|
|
| MCA1210EL | F | 0.5 mg |
|
|
| MCA1210PB | F | 100 Tests/1ml |
|
|
| MCA1210 | F | 100 Tests |
|
|
| MCA1210PE | F | 100 Tests |
|
|
| MCA1210P647 | F | 100 Tests/1ml |
|
|
| MCA1210A700 | F | 100 Tests/1ml |
|
Mouse IgG2a Negative Control is suitable for use as a matched isotype negative control reagent to assess the level of non-specific binding of mouse IgG2a monoclonal antibodies to rat cells. This antibody has been tested and found to be negative on the following rat cells – peripheral blood leucocytes, thymocytes, splenocytes and macrophages.
Mouse IgG2a Negative Control (MCA1210) recognizes the human CD45 antigen and therefore human leucocytes may be used as a positive control for this product.
THIS PRODUCT IS NOT SUITABLE FOR USE AS A NEGATIVE CONTROL ON HUMAN TISSUES.
Mouse IgG2a Negative Control (MCA1210) recognizes the human CD45 antigen and therefore human leucocytes may be used as a positive control for this product.
THIS PRODUCT IS NOT SUITABLE FOR USE AS A NEGATIVE CONTROL ON HUMAN TISSUES.
Product Details
- Target Species
- Negative Control
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to Pacific Blue® - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Product Form
- Purified IgG conjugated to R. Phycoerythrin Alexa Fluor®750 - lyophilized
- Product Form
- Purified IgG conjugated to Alexa Fluor® 700 - liquid
- Reconstitution
- Reconstitute with 1 ml distilled water
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by ion exchange chromatography
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by ion exchange chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Human T lymphocytes.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the NSI mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 12 months from date of reconstitution.
- Guarantee
- 6 months from date of reconstitution
- Guarantee
- 18 months from date of despatch.
More Information
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of Mouse IgG2a Negative Control antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | * | ||
| Flow Cytometry | * | ||
| Flow Cytometry | * | ||
| Flow Cytometry | * | ||
| Flow Cytometry | * | ||
| Flow Cytometry | * | ||
| Flow Cytometry | * | ||
| Flow Cytometry | * | ||
| Flow Cytometry | * | ||
| Flow Cytometry | * | ||
| Flow Cytometry | * |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.
* It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody.
* It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.
* It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody.
* It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.
* It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody.
* It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.
*It is suggested that the user adjusts the concentration of this reagent to be the same as the test reagent.
*It is suggested that the user adjusts the concentration of this reagent to be the same as the test reagent.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.
* It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody.
* It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.
*It is recommended that the user dilutes the antibody in their own system to a concentration equivalent to their test antibody.
*It is recommended that the user dilutes the antibody in their own system to a concentration equivalent to their test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.
* It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody.
* It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.
*It is recommended that the user dilutes the antibody in their own system to a concentration equivalent to their test antibody.
*It is recommended that the user dilutes the antibody in their own system to a concentration equivalent to their test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.
* It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody.
* It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.
* It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody.
* It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *It is recommended that the user dilutes the antibody to a concentration equivalent to their test reagent.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Product Specific References
References for Mouse IgG2a Negative Control antibody
-
Fijak, M. et al. (2015) Influence of Testosterone on Inflammatory Response in Testicular Cells and Expression of Transcription Factor Foxp3 in T Cells.
Am J Reprod Immunol. 74 (1): 12-25.
