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Mouse IgG2a Negative Control antibody

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Mouse IgG2a Negative Control Antibody - Human thumbnail image 1 Mouse IgG2a Negative Control Antibody - Human thumbnail image 2 Mouse IgG2a Negative Control Antibody - Human thumbnail image 3 Mouse IgG2a Negative Control Antibody - Human thumbnail image 4 Mouse IgG2a Negative Control Antibody - Human thumbnail image 5 Mouse IgG2a Negative Control Antibody - Human thumbnail image 6
Mouse IgG2a Negative Control Antibody - Human gallery image 1
Figure A. FITC conjugated mouse anti rat CD3 (MCA772F) and RPE conjugated mouse IgG2a isotype control (MCA1210PE). Figure B. FITC conjugated mouse anti rat CD3 (MCA772F) and RPE conjugated mouse anti rat CD2 (MCA154PE). All experiments performed on red cell lysed rat blood gated on mononuclear cells.
Mouse IgG2a Negative Control Antibody - Human gallery image 2
Figure A. A647 conjugated mouse anti rat CD4 (MCA153A647) and RPE conjugated mouse IgG2a isotype control (MCA1210PE). Figure B. A647 conjugated mouse anti rat CD4 (MCA153A647) and RPE conjugated mouse anti rat CD44 (MCA643PE). All experiments performed on red cell lysed rat blood gated on mononuclear cells.
Mouse IgG2a Negative Control Antibody - Human gallery image 3
Figure A. RPE conjugated mouse anti rat CD44 (MCA643PE) and A647 conjugated mouse IgG2a isotype control (MCA1210A647). Figure B. RPE conjugated mouse anti rat CD44 (MCA643PE) and RPE conjugated mouse anti rat CD4 (MCA153A647). All experiments performed on red cell lysed rat blood gated on mononuclear cells.
Mouse IgG2a Negative Control Antibody - Human gallery image 4
Figure A. FITC conjugated mouse anti rat CD45 (MCA43FB) and RPE conjugated mouse IgG2a isotype control (MCA1210PE). Figure B. FITC conjugated mouse anti rat CD45 (MCA43FB) and RPE conjugated mouse anti rat CD49d (MCA2872PE). All experiments performed on red cell lysed rat blood gated on mononuclear cells.
Mouse IgG2a Negative Control Antibody - Human gallery image 5
Figure A. PE conjugated mouse anti rat CD106 (MCA4633PE) and FITC conjugated mouse IgG2a isotype control (MCA1210F). Figure B. PE conjugated mouse anti rat CD106 (MCA4633PE) and FITC conjugated mouse anti rat CD11b (MCA619F). All experiments performed on red cell lysed rat bone marrow gated on mononuclear cells.
Mouse IgG2a Negative Control Antibody - Human gallery image 6
Figure A. PE conjugated mouse anti rat CD172a (MCA620PE) and FITC conjugated mouse IgG2a isotype control (MCA1210F). Figure B. PE conjugated mouse anti rat CD172a (MCA620PE) and FITC conjugated mouse anti rat CD11c (MCA1441F). All experiments performed on rat peritoneal macrophages.

Mouse IgG2a Negative Control:Alexa Fluor® 488

Mouse IgG2a isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a human surface antigen.

Mouse IgG2a Negative Control:Alexa Fluor® 647

Mouse IgG2a isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a human surface antigen.

Mouse IgG2a Negative Control:Preservative Free

Mouse IgG2a isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a human surface antigen.

Mouse IgG2a Negative Control:Biotin

Mouse IgG2a isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a human surface antigen.

Mouse IgG2a Negative Control:FITC

Mouse IgG2a isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a human surface antigen.

Mouse IgG2a Negative Control:Pacific Blue®

Mouse IgG2a isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a human surface antigen.

Mouse IgG2a Negative Control

Mouse IgG2a isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a human surface antigen.

Mouse IgG2a Negative Control:RPE

Mouse IgG2a isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a human surface antigen.

Mouse IgG2a Negative Control:RPE-Alexa Fluor® 647

Mouse IgG2a isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a human surface antigen.

Mouse IgG2a Negative Control:Alexa Fluor® 700

Mouse IgG2a isotype control for measurement of non specific binding of mouse monoclonal antibodies. This antibody recognizes a human surface antigen.

Product Type
Negative/Isotype Control
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA1210A488
Datasheet
F
SDS
100 Tests/1ml loader

MCA1210A647
Datasheet
F
SDS
100 Tests/1ml loader

MCA1210XZ
Datasheet
F
SDS
1 mg loader

MCA1210B
Datasheet
F
SDS
0.1 mg loader

MCA1210F
Datasheet
F
SDS
0.1 mg loader

MCA1210PB
Datasheet
F
SDS
100 Tests/1ml loader

MCA1210
Datasheet
F
SDS
100 Tests loader

MCA1210PE
Datasheet
F
SDS
100 Tests loader

MCA1210P647
Datasheet
F
SDS
100 Tests/1ml loader

MCA1210A700
Datasheet
F
SDS
100 Tests/1ml loader

Mouse IgG2a Negative Control is suitable for use as a matched isotype negative control reagent to assess the level of non-specific binding of mouse IgG2a monoclonal antibodies to rat cells. This antibody has been tested and found to be negative on the following rat cells – peripheral blood leucocytes, thymocytes, splenocytes and macrophages.

Mouse IgG2a Negative Control (MCA1210) recognizes the human CD45 antigen and therefore human leucocytes may be used as a positive control for this product.

THIS PRODUCT IS NOT SUITABLE FOR USE AS A NEGATIVE CONTROL ON HUMAN TISSUES.

Product Details

Target Species
Negative Control
Product Form
Purified IgG conjugated to Alexa Fluor® 488 - liquid
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG conjugated to Pacific Blue® - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Product Form
Purified IgG conjugated to R. Phycoerythrin Alexa Fluor®750 - lyophilized
Product Form
Purified IgG conjugated to Alexa Fluor® 700 - liquid
Reconstitution
Reconstitute with 1 ml distilled water
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by ion exchange chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
None present
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Carrier Free
Yes
Immunogen
Human T lymphocytes.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the NSI mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at -20oC only.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
6 months from date of despatch
Guarantee
12 months from date of despatch

More Information

Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of Mouse IgG2a Negative Control antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry *
Flow Cytometry *
Flow Cytometry *
Flow Cytometry *
Flow Cytometry *
Flow Cytometry *
Flow Cytometry *
Flow Cytometry *
Flow Cytometry *
Flow Cytometry *
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.

* It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.

* It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.

* It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.

*It is suggested that the user adjusts the concentration of this reagent to be the same as the test reagent.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.

* It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.

* It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.

*It is recommended that the user dilutes the antibody in their own system to a concentration equivalent to their test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.

* It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.

* It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *It is recommended that the user dilutes the antibody to a concentration equivalent to their test reagent.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Product Specific References

References for Mouse IgG2a Negative Control antibody

  1. Fijak, M. et al. (2015) Influence of Testosterone on Inflammatory Response in Testicular Cells and Expression of Transcription Factor Foxp3 in T Cells.
    Am J Reprod Immunol. 74 (1): 12-25.

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