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CD68 antibody | ED1

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MCA341PE
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Mouse anti rat CD68 antibody, clone ED1 recognizes the rat ED1 antigen, a heavily glycosylated protein of ~90 -110 kDa, also known as rat CD68 (Dijkstra et al. 1985).

The ED1 antigen is expressed on most macrophages populations, as well as on monocytes and is considered as a pan-macrophage marker in the rat (Damoiseaux et al. 1994). ED1 is expressed predominantly on the lysosomal membrane and lightly on the cell surface (Dijkstra et al. 1985).

The expression of ED1 antigen being predominantly cytoplasmic (Dijkstra et al. 1985), flow cytometry results are improved by the use of a membrane permeabilization procedure, such as Leucoperm, prior to staining.
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Our CD68 (ED1) Antibody has been referenced in >580 publications*


*Based on June 2020 data from CiteAb's antibody search engine.

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
Horse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Rat spleen cells.
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the SP2/0-Ag14 mouse myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

This product should be stored undiluted.

DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 Neat 1/10
  1. 1 Membrane permeabilisation is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive control.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA1209PE F 100 Tests
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List Price Your Price
Description Mouse IgG1 Negative Control:RPE

Source Reference

  1. Dijkstra, C.D. et al. (1985) The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3.
    Immunology. 54 (3): 589-99.

References for CD68 antibody

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Flow Cytometry

Immunofluorescence

Immunohistology - Frozen

Immunohistology - Paraffin

Western Blotting

Synonyms
ED1
RRID
AB_324585
UniProt
Q4FZY1

MCA341PE

154486 167452

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