Ranibizumab antibody | AbD29858_hIgG1

Human anti Ranibizumab

Product Type
Monoclonal Antibody
Clone
AbD29858_hIgG1
Isotype
IgG1
Specificity
Ranibizumab

Product Code Applications Pack Size List Price Your Price Qty
HCA316
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SDS Safety Datasheet SDS
E 0.1 mg loader
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loader
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Human Anti-Ranibizumab Antibody, clone AbD29858_hIgG1 is a non-inhibitory, anti-idiotypic antibody in full immunoglobulin format that specifically recognizes the monoclonal antibody drug ranibizumab and ranibizumab when in complex with recombinant human vascular endothelial growth factor A (VEGF-A). It does not recognize free human VEGF-A or bevacizumab. This antibody can be used to measure levels of free ranibizumab and biosimilars in patient samples.

Clone AbD29858_hIgG1 in full immunoglobulin format can be used to develop a pharmacokinetic (PK) antigen capture format assay to measure free ranibizumab that has been captured via immobilized VEGF-A.

Ranibizumab (Lucentis) is a humanized Fab fragment antibody (VH3-23/kappa1), originating from the same parental mouse antibody as bevacizumab (Avastin); it has 4 amino acid exchanges in the heavy chain and 1 amino acid exchange in the light chain compared to bevacizumab. Ranibizumab was first approved to treat wet age-related macular degeneration (AMD), a common form of age-related vision loss. It is also approved for the treatment of macular edema following retinal vein occlusion, diabetic macular edema, and diabetic retinopathy. Ranibizumab inhibits angiogenesis by binding to VEGF-A, blocking its interaction with VEGF receptors on the surface of endothelial cells, and thus preventing the subsequent growth of new blood vessels.

View a summary of all anti-ranibizumab antibodies

Product Form
Human IgG1 antibody (lambda light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Ranibizumab
Affinity
The monovalent intrinsic affinity of AbD29858_hIgG1 was measured as KD=18 nM by real time, label-free molecular interaction analysis on immobilized VEGF/ranibizumab complex.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Lucentis is a trademark of Genentech.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
Clone AbD29858_hIgG1 can be used in a direct or indirect ELISA on ranibizumab or as a detection reagent for free ranibizumab captured via immobilized VEGF-A.

Protocol: PK antigen capture ELISA to measure free drug

Description Product Code Applications Pack Size List Price Your Price Quantity
Recombinant Human IgG1 Lambda Allotype G1m3 HCA049 E 0.1 mg loader
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loader
Description Recombinant Human IgG1 Lambda Allotype G1m3

Description Product Code Applications Pack Size List Price Your Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
List Price Your Price
Description Hispec Assay Diluent
LYNX Rapid HRP Antibody Conjugation Kit LNK002P CJ 3 Conjugations For 400µg Antibody
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Description LYNX Rapid HRP Antibody Conjugation Kit
Recombinant Human VEGF-A PHP293 E 0.2 mg loader
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loader
Description Recombinant Human VEGF-A

Synonyms
Lucentis
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"