Granulocytes antibody | 2B2
Mouse anti Porcine granulocytes antibody, clone 2B2 (MCA2600) used to identify mature neutrophils in porcine blood by flow cytometry in conjunction with Mouse anti Porcine granulocytes antibody, clone 6D10 (MCA2599GA) expressed on both immature and mature porcine neutrophils.
Image caption:
Gating strategy.
Granulocytes were further sub-divided into immature neutrophils (CD172a+ SSC high 6D10+ 2B2-) and mature neutrophils (CD172a+ SSC high 6D10+ 2B2+).
From: Nguyen DN, Jiang P, Frøkiær H, Heegaard PM, Thymann T, Sangild PT.
Delayed development of systemic immunity in preterm pigs as a model for preterm infants.
Sci Rep. 2016 Nov 10;6:36816.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Pig Granulocytes:FITC
- Product Type
- Monoclonal Antibody
- Clone
- 2B2
- Isotype
- IgG1
- Specificity
- Granulocytes
Antibody Quality and Performance Guarantee
| Mouse anti Pig granulocytes antibody, clone 2B2 recognizes porcine basophils, neutrophils and eosinophils, acting as a reliable tool for their analysis and isolation, without contamination from other cells. Analysis of porcine bone marrow and blood shows that the antigen recognized by clone 2B2 is selectively expressed on mature cells and that it is likely to be a labile conformational epitope. Use of clone 2B2 in conjunction with clone 6D10 product code (MCA2599GA), allows for the discrimination and characterization of different porcine granulocyte lineages and also their developmental stages: 6D10-2B2- early myeloid precursors, 6D10+2B2- immature neutrophils, 6D10+2B2+mature neutrophils and 6D10-2B2+ mature eosinophils and basophils. Clone 2B2 has been shown as suitable for use on cytospins (Pérez et al. 2007). |
- Target Species
- Pig
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin - Immunogen
- Porcine bone marrow hematopoietic cells (BMHC)
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mouse were fused with cells of the Sp2/0 mouse myeloma cell line
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control:FITC | ||||||
References for Granulocytes antibody
-
Nguyen, D.N. et al. (2016) Delayed development of systemic immunity in preterm pigs as a model for preterm infants.
Sci Rep. 6: 36816. -
LeLuduec, J.B. et al. (2016) Intradermal vaccination with un-adjuvanted sub-unit vaccines triggers skin innate immunity and confers protective respiratory immunity in domestic swine.
Vaccine. 34 (7): 914-22. -
Andersen, A.D. et al. (2019) Synbiotics Combined with Glutamine Stimulate Brain Development and the Immune System in Preterm Pigs.
J Nutr. 149 (1): 36-45.
Further Reading
-
Pérez, C. et al. (2007) Phenotypic and functional characterization of porcine granulocyte developmental stages using two new markers.
Dev Comp Immunol. 31 (3): 296-306. -
Piriou-guzylack, L. & Salmon, H. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39 (6): 54.
- RRID
- AB_1102597
MCA2600F
156515
168123
1708
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