Monocyte/Granulocyte antibody | 74-22-15
Mouse anti Pig Monocyte/Granulocyte:RPE
- Product Type
- Monoclonal Antibody
- Clone
- 74-22-15
- Isotype
- IgG1
- Specificity
- Monocyte/Granulocyte
Mouse anti Pig Monocyte/Granulocyte antibody, clone 74-22-15, recognizes 90% of pig monocytes and granulocytes, in addition to 5% of lymphocytes, in peripheral blood. The target of Mouse anti Pig Monocyte/Granulocyte antibody, clone 74-22-15 is CD172a, similarly to clone BL1H7 (Haverson et al. 1994, Blecha et al. 1994). CD172a/SWC3a is expressed on monocytes, dendritic cells, and granulocytes. |
- Target Species
- Pig
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
- Preparation
- Purified IgG prepared by combination of precipitation and chromatography techniques
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
Stabilizing agent (sucrose) - Immunogen
- Fresh dd miniature swine thymocytes
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578 RPE 561nm laser 546 578 - Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/5 |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
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References for Monocyte/Granulocyte antibody
-
Labarque, G.G. et al. (2000) Effect of cellular changes and onset of humoral immunity on the replication of porcine reproductive and respiratory syndrome virus in the lungs of pigs.
J Gen Virol. 81 (Pt 5): 1327-34. -
Blecha, F. et al. (1994) Workshop studies on monoclonal antibodies reactive against porcine myeloid cells.
Vet Immunol Immunopathol. 43 (1-3): 269-72. -
Jarosz, ł. et al. (2021) The Effect of Feed Supplementation with EM Bokashi® Multimicrobial Probiotic Preparation on Selected Parameters of Sow Colostrum and Milk as Indicators of the Specific and Nonspecific Immune Response.
Probiotics Antimicrob Proteins. Oct 01 [Epub ahead of print].
- Synonyms
- CD172a
- SWC3a
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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