Ocrelizumab antibody | AbD50989ia
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using Human Anti-Ocrelizumab Antibody, clone AbD50989ia (HCA401) directly conjugated to HRP at a concentration of 2 μg/mL in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. HRP conjugation was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).
A microtiter plate was coated overnight with ocrelizumab, rituximab, infliximab, human IgG1 kappa control (same allotype as ocrelizumab and rituximab) or human IgG1 lambda control at a concentration of 5 µg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using Human Anti-Ocrelizumab Antibody, clone AbD50989ia (HCA401) directly conjugated to HRP, titrated to the given concentrations in HISPEC diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. HRP conjugation was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).
Figure A. PE conjugated ocrelizumab pre-incubated for 1h with Human Anti-Ocrelizumab Antibody, clone AbD50989ia (HCA401, red) or recombinant Human IgG1 Kappa Allotype G1m3 Antibody, clone AbD18705_hIgG1 isotype control (HCA192G, grey) in the monovalent Fab format in ratio 1:1.
Figure B. PE conjugated ocrelizumab pre-incubated for 1h with Human Anti-Ocrelizumab Antibody, clone AbD50989ia (HCA401, red) or recombinant Human IgG1 Kappa Allotype G1m3 Antibody, clone AbD18705_hIgG1 isotype control (HCA192G, grey) in the monovalent Fab format in ratio 5:1.
All experiments were performed on a Daudi cell line expressing CD20 and incubated for 1 hour with the antibody mix.
A microtiter plate was coated overnight with ocrelizumab at a concentration of 1 µg/ml. After washing and blocking with PBST + 5% BSA, PBST with 10% human serum was added spiked with increasing concentrations of Human Anti-Ocrelizumab Antibody, clone AbD50641ia (HCA400, black), clone AbD50989ia (HCA401, red) or clone AbD47395ia (HCA399, blue). Detection was performed using HRP conjugated ocrelizumab at a concentration of 2 µg/ml in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P). Data are shown as the mean of three measurements.
Human anti Ocrelizumab
- Product Type
- Monoclonal Antibody
- Clone
- AbD50989ia
- Isotype
- IgG1
- Specificity
- Ocrelizumab
Antibody Quality and Performance Guarantee
HCA401
| Human Anti-Ocrelizumab Antibody, clone AbD50989ia is a paratope specific, inhibitory, anti-idiotypic antibody (Type 1) that specifically recognizes the free human monoclonal antibody drug ocrelizumab and its biosimilar products. Consequently, this antibody can be used to measure free ocrelizumab levels in serum from patients. Clone AbD50989ia (HCA401) is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a positive control or calibrator in an anti-drug antibody (ADA) assay. It does not cross-react with the antibody drug rituximab (Rituxan). Ocrelizumab (trade name Ocrevus) is a monoclonal IgG1/kappa antibody (VH3/κ1). In vitro, it binds to CD20 cell surface molecules on target cells thereby inducing immune effector mechanisms such as antibody-dependent cellular phagocytosis (ADCP), antibody-dependent cellular cytotoxicity (ADCC), complement-dependent cytotoxicity (CDC), and apoptosis. In vivo, ocrelizumab selectively and effectively depletes CD20+ B cells. Hence, it is an immunosuppressive drug. Ocrelizumab is used to treat primary progressive multiple sclerosis and relapsing forms of multiple sclerosis in adults (including clinically isolated syndrome, relapsing-remitting disease, and active secondary progressive disease). |
|
- Product Form
- Human IgG1 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Source
- HKB-11
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Ocrelizumab
- Affinity
- The monovalent intrinsic affinity of Human anti-Ocrelizumab Antibody, clone AbD50989ia was measured as KD = 0.7 nM by real time, label-free molecular interaction analysis on immobilized ocrelizumab.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Ocrevus is a registered trademark of Genentech/ Roche and Biogen.
Rituxan is a registered trademark of Genentech and Biogen. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
- This product may be used to develop and calibrate immune response assays to measure the anti-drug antibody (ADA) response in patient sera together with HCA399 and HCA400.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
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| Description | Hispec Assay Diluent | ||||||
| Human anti Ocrelizumab | HCA399 | E | 0.1 mg |
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| Description | Human anti Ocrelizumab | ||||||
| Human anti Ocrelizumab | HCA400 | E | 0.1 mg |
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| Description | Human anti Ocrelizumab | ||||||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody | Log in | |||
| List Price | Your Price | ||||||
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| Description | LYNX Rapid HRP Antibody Conjugation Kit | ||||||
| Human anti Ocrelizumab | TZA044 | E | 0.1 mg | Log in | |||
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| Description | Human anti Ocrelizumab | ||||||
| Human anti Ocrelizumab | TZA045 | E | 0.1 mg | Log in | |||
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| Description | Human anti Ocrelizumab | ||||||
| Human anti Ocrelizumab:HRP | TZA045P | E | 0.1 mg | Log in | |||
| List Price | Your Price | ||||||
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| Description | Human anti Ocrelizumab:HRP | ||||||
- Synonyms
- Ocrevus
HCA401
166856
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