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Ocrelizumab antibody | AbD50641ad

Human anti Ocrelizumab

Product Type
Monoclonal Antibody
Clone
AbD50641ad
Isotype
Fab antibody
Specificity
Ocrelizumab

Product Code Applications Pack Size List Price Your Price Qty
New TZA044
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
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Human Anti-Ocrelizumab Antibody, clone AbD50641ad is a paratope specific, inhibitory, anti-idiotypic antibody (Type 1) that specifically recognizes the free human monoclonal antibody drug ocrelizumab and its biosimilar products. Consequently, this antibody can be used to measure free ocrelizumab levels in serum from patients.

This antibody is available in two formats - a monovalent Fab unconjugated format, clone AbD50641ad (TZA044), which has a SpyTag incorporated. This format can be used as a capture antibody in a pharmacokinetic (PK) bridging ELISA together with AbD50651 as a detection antibody. Clone AbD50641 is also available in full length IgG1 format under product code HCA400 which can be used in an ADA bridging ELISA as a surrogate positive control.

Human Anti-Ocrelizumab Antibody, clone AbD50641ad has been shown to cross-react with the antibody drug rituximab (tradename Rituxan). Ocrelizumab is the humanized form of rituximab. There are changes in at least one of the CDRs and it contains Fc modification.

Ocrelizumab (trade name Ocrevus) is a monoclonal IgG1/kappa antibody (VH3/κ1). In vitro, it binds to CD20 cell surface molecules on target cells thereby inducing immune effector mechanisms such as antibody-dependent cellular phagocytosis (ADCP), antibody-dependent cellular cytotoxicity (ADCC), complement-dependent cytotoxicity (CDC), and apoptosis. In vivo, ocrelizumab selectively and effectively depletes CD20+ B cells. Hence, it is an immunosuppressive drug. Ocrelizumab is used to treat primary progressive multiple sclerosis and relapsing forms of multiple sclerosis in adults (including clinically isolated syndrome, relapsing-remitting disease, and active secondary progressive disease).

Product Form
A monovalent human recombinant Fab (lambda light chain) selected from Bio-Rad’s human antibody phage display libraries. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography
Source
E.coli
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.0095% MIT
Immunogen
Ocrelizumab
Affinity
The monovalent intrinsic affinity of Human anti-Ocrelizumab Antibody, clone AbD50641ad was measured as KD<0.001 nM by real time, label-free molecular interaction analysis on immobilized ocrelizumab.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Ocrevus is a registered trademark of Genentech/ Roche and Biogen.
Rituxan is a registered trademark of Genentech and Biogen.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used in a direct ELISA.
Clone AbD50641ad can be used as the capture antibody for ocrelizumab in a PK bridging ELISA together with clone AbD50651pap (TZA045P) as the detection antibody.

Description Product Code Applications Pack Size List Price Your Price Quantity
SpyTag3 Peptide BLP086 IY 1 mg loader
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Description SpyTag3 Peptide
Hispec Assay Diluent BUF049A E IY 50 ml
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Description Hispec Assay Diluent
Human anti Ocrelizumab HCA399 E 0.1 mg loader
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Description Human anti Ocrelizumab
Human anti Ocrelizumab HCA400 E 0.1 mg loader
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loader
Description Human anti Ocrelizumab
Human anti Ocrelizumab HCA401 E 0.1 mg loader
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Description Human anti Ocrelizumab
Human anti Ocrelizumab TZA045 E 0.1 mg
List Price Your Price
Description Human anti Ocrelizumab
Human anti Ocrelizumab:HRP TZA045P E 0.1 mg
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Description Human anti Ocrelizumab:HRP
BiSpyCatcher2:HRP TZC002P IY 1 mg
List Price Your Price
Description BiSpyCatcher2:HRP

Synonyms
Ocrevus
Licensed Use
For in vitro. research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
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