TOLLIP antibody | Kimmy-1
Rat anti TOLLIP antibody clone Kimmy-1 recognizes Toll-interacting protein (TOLLIP), an ubiquitin binding protein that plays an important role in regulating the innate immune response.
TOLLIP, as the name implies, interacts with a number of Toll-like receptors thereby mediating the regulation of the autophagic clearance and NF-kappaB pathways. The latter pathway, TOLLIP mainly regulates through controlling phosphorylation levels of the IRAK1 kinase. TOLLIP deficiencies have been implicated in increased tuberculosis susceptibility (Sha et al. 2012).
TOLLIP, as the name implies, interacts with a number of Toll-like receptors thereby mediating the regulation of the autophagic clearance and NF-kappaB pathways. The latter pathway, TOLLIP mainly regulates through controlling phosphorylation levels of the IRAK1 kinase. TOLLIP deficiencies have been implicated in increased tuberculosis susceptibility (Sha et al. 2012).
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- Immunogen
- Recombinant mouse TOLLIP
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9QZ06
- Entrez Gene
- Tollip
- GO Terms
- GO:0005515 protein binding
- GO:0005737 cytoplasm
- GO:0006954 inflammatory response
- GO:0016310 phosphorylation
- GO:0045087 innate immune response
- Regulatory
- For research purposes only
Applications of TOLLIP antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunoprecipitation | |||
| Western Blotting | 5 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rat anti TOLLIP recognizes a band of ~30 kDa by western blot.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
|
|
| Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
|
|
| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
|
|
| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
|
|
| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
|
|
| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Further Reading
-
Shah, J.A.
. (2012) Human TOLLIP regulates TLR2 and TLR4 signaling and its polymorphisms are associated with susceptibility to tuberculosis.
J Immunol. 189(4):1737-46
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