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TOLLIP antibody | Kimmy-1

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Anti TOLLIP Antibody, clone Kimmy-1 gallery image 1
25 &mug murine EL4 whole cell lysate (lanes 2, and 3) was loaded onto a 4-15% Criterion™ TGX-Stain-Free™ Gel and transferred onto a nitrocellulose membrane. The membrane was blocked with Block ACE (BUF029) and probed with rat anti TOLLIP antibody (MCA6034) at 5ug/ml in Block ACE wash (Lane 2) for 1 hour at room temperature. The membrane was washed 3x for 10 minutes with 5 ml Block ACE wash before addition of the HRP-conjugated goat anti-Rat IgG (STAR72) secondary antibody at 1/10,000 (Lanes 1-3). The membrane was washed 4x for 5 minutes with Block ACE wash and 2x in 5 ml PBS before developing with Clarity ECL on the Bio-Rad Chemidoc. Lane 3 is a secondary only antibody control.

Rat anti TOLLIP

Product Type
Monoclonal Antibody
Clone
Kimmy-1
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA6034
Datasheet
IP WB
SDS
50 µg loader

Rat anti TOLLIP antibody clone Kimmy-1 recognizes Toll-interacting protein (TOLLIP), an ubiquitin binding protein that plays an important role in regulating the innate immune response.

TOLLIP, as the name implies, interacts with a number of Toll-like receptors thereby mediating the regulation of the autophagic clearance and NF-kappaB pathways. The latter pathway, TOLLIP mainly regulates through controlling phosphorylation levels of the IRAK1 kinase. TOLLIP deficiencies have been implicated in increased tuberculosis susceptibility (Sha et al. 2012).

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
Recombinant mouse TOLLIP
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q9QZ06
Entrez Gene
Tollip
GO Terms
GO:0005515 protein binding
GO:0005737 cytoplasm
GO:0006954 inflammatory response
GO:0016310 phosphorylation
GO:0045087 innate immune response
Regulatory
For research purposes only

Applications of TOLLIP antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 5 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rat anti TOLLIP recognizes a band of ~30 kDa by western blot.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

Further Reading

  1. Shah, J.A. . (2012) Human TOLLIP regulates TLR2 and TLR4 signaling and its polymorphisms are associated with susceptibility to tuberculosis.
    J Immunol. 189(4):1737-46

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