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MHC Class II I-A/I-E antibody | M5/114.15.2

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Anti Mouse MHC Class II I-A/I-E Antibody, clone M5/114.15.2 gallery image 1
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A lack of CD4+ T cells or of IFNγ increases MHC II+ cell infection. a. Naive mouse lungs were stained for AEC2 (SPC+), AM (CD11c+), and MHC II. The filled arrow shows an MHC II+ AM. White / red co-localization appears as pink. Most MHC II+ cells were AEC2 (open arrows). Red / green co-localization appears as yellow. Bars show mean ± SEM of counts for 3 fields of view per section, for 2 sections from each of 2 mice. b. Naïive and d5 infected lungs were stained for SPC, MHC II and viral antigens (MHV). In infected lungs, open arrows show MHC II+ AEC2, which are uninfected (MHV-); white filled arrows show infected MHC II+ cells, which are SPC-; the grey filled arrow shows a rare MHV+ AEC2 (colocalization appears as white), which is MHC II-. Fig 5a shows that in naïve mice 70% of MHC II+ lung cells were SPC+. After infection, significantly fewer were SPC+ (p<0.001 by Student's 2 tailed unpaired t test), and negligible numbers of MHV+MHC II+ cells (<5%) were SPC+. Bars show mean ± SEM of counts for 3 fields of view per section, across 3 sections from each of 2 mice. c. Mice were depleted of CD8+ T cells (αCD8), given optionally either IFNγ neutralizing (αCD8αIFNγ) or CD4+ T cell depleting antibodies (αCD8αCD4), then given MuHV-4 i.n. (104 p.f.u.). At d9 lungs were stained for MHV and MHC II. Arrows show dual positive cells. d. For samples as illustrated in c, cells were counted for 3 fields of view per section, across 3 sections from each of 3 mice per group. The proportion of MHV+ cells that were MHC II+ was significantly increased by αCD4 (p<0.05) but not by αIFNγ (p>0.2). e. MHC II+MHV+ cells of mice treated as in b were identified as CD11c+ or CD11c-. Cells were counted for 3 fields of view per section, across 3 sections from each of 3 mice per group. αIFNγ significantly increased MHV+MHC II+CD11c+ (p<0.01) but not MHV+MHC II+CD11c- cell numbers (p>0.3). αCD4 significantly increased both (p<0.01, p<0.05). f. Example images are shown for the samples counted in e. Arrows show MHV+CD11c+ cells. g. Staining the same samples for SPC showed that essentially no MHV+MHC II+CD11c- cells were AEC2.

From: Tan CSE, Lawler C, Stevenson PG (2017)
CD8+ T cell evasion mandates CD4+ T cell control of chronic gamma-herpesvirus infection.
PLoS Pathog 13(4): e1006311.
doi: 10.1371/journal.ppat.1006311
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Rat anti Mouse MHC Class II I-A/I-E:FITC

Rat anti Mouse MHC Class II I-A/I-E

Rat anti Mouse MHC Class II I-A/I-E:RPE

Product Type
Monoclonal Antibody
Clone
M5/114.15.2
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
MCA6078F
Datasheet
F
SDS
0.1 mg loader

MCA6078GA
Datasheet
F
SDS
0.1 mg loader

MCA6078PE
Datasheet
F
SDS
0.1 mg loader

Rat anti Mouse MHC class II I-A/I-E antibody, clone M5/114.15.2 recognizes a polymorphic determinant shared by the mouse I-Ab/d/q and I-Ed/k MHC class II alloantigens expressed by mice of the H-2p/r/q/b/d/u haplotypes. This antibody does not recognize the I-Af/k/s MHC class II alloantigens.

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region. MHC class II molecules are expressed by dendritic cells, B-cells and macrophages.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Activated C57BL/6 mouse spleen cells.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 0.2 mg/ml
Fusion Partners
Immunized (BN x Lewis)F1 rat spleen cells were fused with the NS1 myeloma cell line.

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
Q31161
Regulatory
For research purposes only

Applications of MHC Class II I-A/I-E antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/20
Flow Cytometry 1/20
Flow Cytometry 1/5 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Product Specific References

Source Reference

  1. Bhattacharya A et al. (1981) A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication.
    J Immunol. 127 (6): 2488-95.

References for MHC Class II I-A/I-E antibody

  1. Tan, C.S.E. et al. (2017) CD8+ T cell evasion mandates CD4+ T cell control of chronic gamma-herpesvirus infection.
    PLoS Pathog. 13 (4): e1006311.
  2. Curtsinger JM et al. (1999) Inflammatory cytokines provide a third signal for activation of naive CD4+ and CD8+ T cells.
    J Immunol. 162 (6): 3256-62.
  3. Miyazaki, T. et al. (1996) Mice lacking H2-M complexes, enigmatic elements of the MHC class II peptide-loading pathway.
    Cell. 84 (4): 531-41.
  4. Anderson, M.S. & Miller, J. (1992) Invariant chain can function as a chaperone protein for class II major histocompatibility complex molecules.
    Proc Natl Acad Sci U S A. 89 (6): 2282-6.
  5. Lawler, C. & Stevenson, P.G. (2019) A CD4+ T cell/NK cell axis of γ-herpesvirus control.
    J Virol. Nov 06 [Epub ahead of print].

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