MHC Class II I-A/I-E antibody | M5/114.15.2
Insert meta-description and applications
Image caption:
A lack of CD4+ T cells or of IFNγ increases MHC II+ cell infection. a. Naive mouse lungs were stained for AEC2 (SPC+), AM (CD11c+), and MHC II. The filled arrow shows an MHC II+ AM. White / red co-localization appears as pink. Most MHC II+ cells were AEC2 (open arrows). Red / green co-localization appears as yellow. Bars show mean ± SEM of counts for 3 fields of view per section, for 2 sections from each of 2 mice. b. Naïive and d5 infected lungs were stained for SPC, MHC II and viral antigens (MHV). In infected lungs, open arrows show MHC II+ AEC2, which are uninfected (MHV-); white filled arrows show infected MHC II+ cells, which are SPC-; the grey filled arrow shows a rare MHV+ AEC2 (colocalization appears as white), which is MHC II-. Fig 5a shows that in naïve mice 70% of MHC II+ lung cells were SPC+. After infection, significantly fewer were SPC+ (p<0.001 by Student's 2 tailed unpaired t test), and negligible numbers of MHV+MHC II+ cells (<5%) were SPC+. Bars show mean ± SEM of counts for 3 fields of view per section, across 3 sections from each of 2 mice. c. Mice were depleted of CD8+ T cells (αCD8), given optionally either IFNγ neutralizing (αCD8αIFNγ) or CD4+ T cell depleting antibodies (αCD8αCD4), then given MuHV-4 i.n. (104 p.f.u.). At d9 lungs were stained for MHV and MHC II. Arrows show dual positive cells. d. For samples as illustrated in c, cells were counted for 3 fields of view per section, across 3 sections from each of 3 mice per group. The proportion of MHV+ cells that were MHC II+ was significantly increased by αCD4 (p<0.05) but not by αIFNγ (p>0.2). e. MHC II+MHV+ cells of mice treated as in b were identified as CD11c+ or CD11c-. Cells were counted for 3 fields of view per section, across 3 sections from each of 3 mice per group. αIFNγ significantly increased MHV+MHC II+CD11c+ (p<0.01) but not MHV+MHC II+CD11c- cell numbers (p>0.3). αCD4 significantly increased both (p<0.01, p<0.05). f. Example images are shown for the samples counted in e. Arrows show MHV+CD11c+ cells. g. Staining the same samples for SPC showed that essentially no MHV+MHC II+CD11c- cells were AEC2.
From: Tan CSE, Lawler C, Stevenson PG (2017)
CD8+ T cell evasion mandates CD4+ T cell control of chronic gamma-herpesvirus infection.
PLoS Pathog 13(4): e1006311.
doi: 10.1371/journal.ppat.1006311
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
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IF ResetRat anti Mouse MHC Class II I-A/I-E
- Product Type
- Monoclonal Antibody
- Clone
- M5/114.15.2
- Isotype
- IgG2b
- Specificity
- MHC Class II I-A/I-E
Antibody Quality and Performance Guarantee
| Rat anti Mouse MHC class II I-A/I-E antibody, clone M5/114.15.2 recognizes a polymorphic determinant shared by the mouse I-Ab/d/q and I-Ed/k MHC class II alloantigens expressed by mice of the H-2p/r/q/b/d/u haplotypes. This antibody does not recognize the I-Af/k/s MHC class II alloantigens. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region. MHC class II molecules are expressed by dendritic cells, B-cells and macrophages. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Activated C57BL/6 mouse spleen cells.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Fusion Partners
- Immunized (BN x Lewis)F1 rat spleen cells were fused with the NS1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/20 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Source Reference
-
Bhattacharya A et al. (1981) A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication.
J Immunol. 127 (6): 2488-95.
References for MHC Class II I-A/I-E antibody
-
Anderson, M.S. & Miller, J. (1992) Invariant chain can function as a chaperone protein for class II major histocompatibility complex molecules.
Proc Natl Acad Sci U S A. 89 (6): 2282-6. -
Miyazaki, T. et al. (1996) Mice lacking H2-M complexes, enigmatic elements of the MHC class II peptide-loading pathway.
Cell. 84 (4): 531-41. -
Curtsinger JM et al. (1999) Inflammatory cytokines provide a third signal for activation of naive CD4+ and CD8+ T cells.
J Immunol. 162 (6): 3256-62. -
Tan, C.S.E. et al. (2017) CD8+ T cell evasion mandates CD4+ T cell control of chronic gamma-herpesvirus infection.
PLoS Pathog. 13 (4): e1006311. -
Lawler, C. & Stevenson, P.G. (2019) A CD4+ T cell/NK cell axis of γ-herpesvirus control.
J Virol. Nov 06 [Epub ahead of print].
- UniProt
- Q31161
MCA6078GA
166106
180208
180227
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