MHC Class II I-A/I-E antibody | M5/114.15.2

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Rat anti Mouse MHC Class II I-A/I-E:FITC

Rat anti Mouse MHC Class II I-A/I-E

Rat anti Mouse MHC Class II I-A/I-E:RPE

Product Type
Monoclonal Antibody
Clone
M5/114.15.2
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA6078F F 0.1 mg
MCA6078GA F 0.1 mg
MCA6078PE F 0.1 mg
Rat anti Mouse MHC class II I-A/I-E antibody, clone M5/114.15.2 recognizes a polymorphic determinant shared by the mouse I-Ab/d/q and I-Ed/k MHC class II alloantigens expressed by mice of the H-2p/r/q/b/d/u haplotypes. This antibody does not recognize the I-Af/k/s MHC class II alloantigens.

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region. MHC class II molecules are expressed by dendritic cells, B-cells and macrophages.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Activated C57BL/6 mouse spleen cells
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 0.2 mg/ml
Fusion Partners
Immunized (BN x Lewis)F1 rat speen cells were fused with the NS1 myeloma cell line.

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch
Shelf Life
12 months from date of despatch
Shelf Life
12 months from date of despatch

More Information

UniProt
Q31161 Related reagents
Regulatory
For research purposes only

Applications of MHC Class II I-A/I-E antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/20
Flow Cytometry 1/20
Flow Cytometry 1/5 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Product Specific References

Source Reference

  1. Bhattacharya A et al. (1981) A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication.
    J Immunol. 127 (6): 2488-95.

References for MHC Class II I-A/I-E antibody

  1. Tan, C.S.E. et al. (2017) CD8+ T cell evasion mandates CD4+ T cell control of chronic gamma-herpesvirus infection.
    PLoS Pathog. 13 (4): e1006311.
  2. Curtsinger JM et al. (1999) Inflammatory cytokines provide a third signal for activation of naive CD4+ and CD8+ T cells.
    J Immunol. 162 (6): 3256-62.
  3. Miyazaki, T. et al. (1996) Mice lacking H2-M complexes, enigmatic elements of the MHC class II peptide-loading pathway.
    Cell. 84 (4): 531-41.
  4. Anderson, M.S. & Miller, J. (1992) Invariant chain can function as a chaperone protein for class II major histocompatibility complex molecules.
    Proc Natl Acad Sci U S A. 89 (6): 2282-6.