MHC Class II I-A/I-E antibody | M5/114.15.2
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Rat anti Mouse MHC Class II I-A/I-E:FITC
Rat anti Mouse MHC Class II I-A/I-E
Rat anti Mouse MHC Class II I-A/I-E:RPE
- Product Type
- Monoclonal Antibody
- Clone
- M5/114.15.2
- Isotype
- IgG2b
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| MCA6078F | F | 0.1 mg | ||||
| MCA6078GA | F | 0.1 mg | ||||
| MCA6078PE | F | 0.1 mg |
Rat anti Mouse MHC class II I-A/I-E antibody, clone M5/114.15.2 recognizes a polymorphic determinant shared by the mouse I-Ab/d/q and I-Ed/k MHC class II alloantigens expressed by mice of the H-2p/r/q/b/d/u haplotypes. This antibody does not recognize the I-Af/k/s MHC class II alloantigens.
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region. MHC class II molecules are expressed by dendritic cells, B-cells and macrophages.
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region. MHC class II molecules are expressed by dendritic cells, B-cells and macrophages.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Preparation
- Purified IgG prepared by affinity chromatography
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Activated C57BL/6 mouse spleen cells
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.2 mg/ml
- Fusion Partners
- Immunized (BN x Lewis)F1 rat speen cells were fused with the NS1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of despatch
- Shelf Life
- 12 months from date of despatch
- Shelf Life
- 12 months from date of despatch
More Information
- UniProt
- Q31161 Related reagents
- Regulatory
- For research purposes only
Applications of MHC Class II I-A/I-E antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/20 | ||
| Flow Cytometry | 1/20 | ||
| Flow Cytometry | 1/5 | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | 1 ml | C E P WB | ||
| Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | 0.5 mg | C E IF P WB | ||
| Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | 0.1 mg | F IF WB | ||
| Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | 1 mg | F | ||
| Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | 1 mg | C E P RE | ||
| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | 0.5 ml | F | ||
| Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) | STAR71D649GA | 0.1 mg | F IF | ||
| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | 0.1 mg | F IF WB | ||
| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | 0.5 mg | C E P | ||
| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | 0.5 ml | F |
Product Specific References
Source Reference
-
Bhattacharya A et al. (1981) A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication.
J Immunol. 127 (6): 2488-95.
References for MHC Class II I-A/I-E antibody
-
Tan, C.S.E. et al. (2017) CD8+ T cell evasion mandates CD4+ T cell control of chronic gamma-herpesvirus infection.
PLoS Pathog. 13 (4): e1006311. -
Curtsinger JM et al. (1999) Inflammatory cytokines provide a third signal for activation of naive CD4+ and CD8+ T cells.
J Immunol. 162 (6): 3256-62. -
Miyazaki, T. et al. (1996) Mice lacking H2-M complexes, enigmatic elements of the MHC class II peptide-loading pathway.
Cell. 84 (4): 531-41. -
Anderson, M.S. & Miller, J. (1992) Invariant chain can function as a chaperone protein for class II major histocompatibility complex molecules.
Proc Natl Acad Sci U S A. 89 (6): 2282-6.
