MHC Class II I-A/I-E antibody | M5/114.15.2
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Rat anti Mouse MHC Class II I-A/I-E
- Product Type
- Monoclonal Antibody
- MHC Class II I-A/I-E
|Rat anti Mouse MHC class II I-A/I-E antibody, clone M5/114.15.2 recognizes a polymorphic determinant shared by the mouse I-Ab/d/q and I-Ed/k MHC class II alloantigens expressed by mice of the H-2p/r/q/b/d/u haplotypes. This antibody does not recognize the I-Af/k/s MHC class II alloantigens.
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region. MHC class II molecules are expressed by dendritic cells, B-cells and macrophages.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Activated C57BL/6 mouse spleen cells.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Fusion Partners
- Immunized (BN x Lewis)F1 rat spleen cells were fused with the NS1 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Bhattacharya A et al. (1981) A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication.
J Immunol. 127 (6): 2488-95.
References for MHC Class II I-A/I-E antibody
Anderson, M.S. & Miller, J. (1992) Invariant chain can function as a chaperone protein for class II major histocompatibility complex molecules.
Proc Natl Acad Sci U S A. 89 (6): 2282-6.
Miyazaki, T. et al. (1996) Mice lacking H2-M complexes, enigmatic elements of the MHC class II peptide-loading pathway.
Cell. 84 (4): 531-41.
Curtsinger JM et al. (1999) Inflammatory cytokines provide a third signal for activation of naive CD4+ and CD8+ T cells.
J Immunol. 162 (6): 3256-62.
Tan, C.S.E. et al. (2017) CD8+ T cell evasion mandates CD4+ T cell control of chronic gamma-herpesvirus infection.
PLoS Pathog. 13 (4): e1006311.
Lawler, C. & Stevenson, P.G. (2019) A CD4+ T cell/NK cell axis of γ-herpesvirus control.
J Virol. Nov 06 [Epub ahead of print].
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