MHC Class II I-A/I-E antibody | M5/114.15.2
Rat anti Mouse MHC Class II I-A/I-E:FITC
Rat anti Mouse MHC Class II I-A/I-E
Rat anti Mouse MHC Class II I-A/I-E:RPE
- Product Type
- Monoclonal Antibody
- Clone
- M5/114.15.2
- Isotype
- IgG2b
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA6078F | F | 0.1 mg |
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MCA6078GA | F | 0.1 mg |
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MCA6078PE | F | 0.1 mg |
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The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region. MHC class II molecules are expressed by dendritic cells, B-cells and macrophages.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Preparation
- Purified IgG prepared by affinity chromatography
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Activated C57BL/6 mouse spleen cells.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.2 mg/ml
- Fusion Partners
- Immunized (BN x Lewis)F1 rat spleen cells were fused with the NS1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q31161
- Regulatory
- For research purposes only
Applications of MHC Class II I-A/I-E antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/20 | ||
Flow Cytometry | 1/20 | ||
Flow Cytometry | 1/5 | 1/10 |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
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Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
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Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
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Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
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Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
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Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
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Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
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Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
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Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
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Product Specific References
Source Reference
-
Bhattacharya A et al. (1981) A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication.
J Immunol. 127 (6): 2488-95.
References for MHC Class II I-A/I-E antibody
-
Tan, C.S.E. et al. (2017) CD8+ T cell evasion mandates CD4+ T cell control of chronic gamma-herpesvirus infection.
PLoS Pathog. 13 (4): e1006311. -
Curtsinger JM et al. (1999) Inflammatory cytokines provide a third signal for activation of naive CD4+ and CD8+ T cells.
J Immunol. 162 (6): 3256-62. -
Miyazaki, T. et al. (1996) Mice lacking H2-M complexes, enigmatic elements of the MHC class II peptide-loading pathway.
Cell. 84 (4): 531-41. -
Anderson, M.S. & Miller, J. (1992) Invariant chain can function as a chaperone protein for class II major histocompatibility complex molecules.
Proc Natl Acad Sci U S A. 89 (6): 2282-6. -
Lawler, C. & Stevenson, P.G. (2019) A CD4+ T cell/NK cell axis of γ-herpesvirus control.
J Virol. Nov 06 [Epub ahead of print].
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