MHC Class II I-A/I-E antibody | M5/114.15.2

Rat anti Mouse MHC Class II I-A/I-E

Product Type
Monoclonal Antibody
Clone
M5/114.15.2
Isotype
IgG2b
Specificity
MHC Class II I-A/I-E

Product Code Applications Pack Size List Price Your Price Qty
MCA6078GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
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loader
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Rat anti Mouse MHC class II I-A/I-E antibody, clone M5/114.15.2 recognizes a polymorphic determinant shared by the mouse I-Ab/d/q and I-Ed/k MHC class II alloantigens expressed by mice of the H-2p/r/q/b/d/u haplotypes. This antibody does not recognize the I-Af/k/s MHC class II alloantigens.

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region. MHC class II molecules are expressed by dendritic cells, B-cells and macrophages.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Activated C57BL/6 mouse spleen cells.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Fusion Partners
Immunized (BN x Lewis)F1 rat spleen cells were fused with the NS1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/20
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

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Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
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Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
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Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)

Source Reference

  1. Bhattacharya A et al. (1981) A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication.
    J Immunol. 127 (6): 2488-95.

References for MHC Class II I-A/I-E antibody

  1. Tan, C.S.E. et al. (2017) CD8+ T cell evasion mandates CD4+ T cell control of chronic gamma-herpesvirus infection.
    PLoS Pathog. 13 (4): e1006311.
  2. Curtsinger JM et al. (1999) Inflammatory cytokines provide a third signal for activation of naive CD4+ and CD8+ T cells.
    J Immunol. 162 (6): 3256-62.
  3. Miyazaki, T. et al. (1996) Mice lacking H2-M complexes, enigmatic elements of the MHC class II peptide-loading pathway.
    Cell. 84 (4): 531-41.
  4. Anderson, M.S. & Miller, J. (1992) Invariant chain can function as a chaperone protein for class II major histocompatibility complex molecules.
    Proc Natl Acad Sci U S A. 89 (6): 2282-6.
  5. Lawler, C. & Stevenson, P.G. (2019) A CD4+ T cell/NK cell axis of γ-herpesvirus control.
    J Virol. Nov 06 [Epub ahead of print].

UniProt
Q31161
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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