MHC Class II H-2I-Ak/D/B/Q/R antibody | ER-TR3
Rat anti Mouse MHC Class II antibody, clone ER-TR3 (MCA2401) used to label MHC-II expressing microglia by immunofluorescence.
Image caption:
Immunostaining of neonatal brain sections with classical and alternative microglia phenotypic markers. Microglial cells (Iba1 +) show increased expression of the classical activation markers MHCII (g–l) after systemic LPS injection. Their expression is significantly diminished when the pups are pre-treated with anti-CD36 antibody ( j–l).
From: Gadagkar SG, Lalancette-Hébert M, Thammisetty SS, Vexler ZS, Kriz J.
CD36 neutralisation blunts TLR2-IRF7 but not IRF3 pathway in neonatal mouse brain and immature human microglia following innate immune challenge.
Sci Rep. 2023 Feb 9;13(1):2304.
doi: 10.1038/s41598-023-29423-0.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
IF ResetRat anti Mouse MHC Class II H-2I-Ak/D/B/Q/R
- Product Type
- Monoclonal Antibody
- Clone
- ER-TR3
- Isotype
- IgG2b
- Specificity
- MHC Class II H-2I-Ak/D/B/Q/R
Antibody Quality and Performance Guarantee
| Rat anti Mouse MHC Class II H-2I-Ak/D/B/Q/R antibody, clone ER-TR3 recognizes a polymorphic determinant of the mouse H-2I-A MHC class II antigen, reacting with haplotypes k, d, b, q and r. Mouse strains with the haplotypes s and f are not recognized by this antibody. Mouse MHC class II antigens are expressed by dendritic cells, B cells and macrophages. In thymus tissues, clone ER-TR3 stains cortical and medullary stromal cells. The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the H-2 region. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Isolated C3H thymic stromal cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cells from immunized rats were fused with cells of the mouse P3-X63-Ag8.563 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
||
| Immunohistology - Frozen | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
Source Reference
-
Van Vliet, E. et al. (1984) Monoclonal antibodies to stromal cell types of the mouse thymus.
Eur J Immunol. 14 (6): 524-9.
References for MHC Class II H-2I-Ak/D/B/Q/R antibody
-
Huiskamp, R. et al. (1985) Repopulation of the mouse thymus after sublethal fission neutron irradiation. II. Sequential changes in the thymic microenvironment.
J Immunol. 134 (4): 2170-8. -
Van Vliet, E. et al. (1985) Stromal cell types in the developing thymus of the normal and nude mouse embryo.
Eur J Immunol. 15 (7): 675-81. -
Matsuno, K. et al. (2002) Kupffer cell-mediated recruitment of dendritic cells to the liver crucial for a host defense.
Dev Immunol. 9 (3): 143-9. -
Gadagkar, S.G. et al. (2023) CD36 neutralisation blunts TLR2-IRF7 but not IRF3 pathway in neonatal mouse brain and immature human microglia following innate immune challenge.
Sci Rep. 13 (1): 2304.
- RRID
- AB_877512
MCA2401
0215
152455
168803
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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