MHC Class I H-2b/D/P/Q/w16 antibody | ER-HR52
Rat anti Mouse MHC Class I H-2b/D/P/Q/w16 antibody, clone ER-HR52 (MCA2398) used for the identification of MHC class I expressing cells in mouse brain by immunofluorescence.
Image caption:
Dynamic expression of H-2Kb/Db in mouse hippocampus.
(A) At P15, MHCI protein signals (green) were overlapped with those of NeuN (red, a marker for neurons) but not with GFAP (red, a marker for astrocytes). Scale bar: 50 μm. (B) The mRNA of H-2Kb and H-2Db was dynamically expressed in mouse hippocampus. Data was shown as ratio compared with P4 and was calculated from three independent experiments. (C) Western blot analysis depicting the expression of MHC-I protein during the development stages of hippocampus. (D) Expression of MHC-I protein was quantified as the ratio of band density to that of Tuj1. Data was presented as ratio compared with P4 and was calculated from three independent experiments.
From: Lv D, Shen Y, Peng Y, Liu J, Miao F, Zhang J (2015) Neuronal MHC Class I Expression Is Regulated by Activity Driven Calcium Signaling.
PLoS ONE 10(8): e0135223.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse MHC Class I H-2b/D/P/Q/w16 antibody, clone ER-HR52 recognizes a polymorphic epitope present on murine MHC class I molecules, which are expressed at varying levels on the majority of nucleated cells.
Clone ER-HR52 specifically recognizes mouse strains with the haplotypes H-2b, w16 and H-2d, p, q. Mouse strains with the haplotypes H-2f, r, s, w17, w23, w27 show weak reactivity with this antibody.
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region.
Clone ER-HR52 specifically recognizes mouse strains with the haplotypes H-2b, w16 and H-2d, p, q. Mouse strains with the haplotypes H-2f, r, s, w17, w23, w27 show weak reactivity with this antibody.
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Adherent FI (CBAXBL) bone marrow stromal cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cells from immunised rats were fused with cells of the mouse P3-X63-Ag8.563 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of MHC Class I H-2b/D/P/Q/w16 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/50 | 1/200 | |
| Immunofluorescence | |||
| Immunohistology - Frozen |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
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| Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
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| Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
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| Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
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| Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
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| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
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| Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) | STAR71D650 | F IF | 0.1 mg |
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| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
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| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
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| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
|
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
|
Product Specific References
References for MHC Class I H-2b/D/P/Q/w16 antibody
-
Medana, I. et al. (2001) Transection of major histocompatibility complex class I-induced neurites by cytotoxic T lymphocytes.
Am J Pathol. 159 (3): 809-15. -
Lv, D. et al. (2015) Neuronal MHC Class I Expression Is Regulated by Activity Driven Calcium Signaling.
PLoS One. 10 (8): e0135223. -
Himoudi, N. et al. (2009) Migratory and antigen presentation functions of IFN-producing killer dendritic cells.
Cancer Res. 69 (16): 6598-606. -
Liu, J. et al. (2015) Spatial-Temporal Expression of Non-classical MHC Class I Molecules in the C57 Mouse Brain.
Neurochem Res. 40 (7): 1487-96. -
Liu, J. et al. (2013) The expression pattern of classical MHC class I molecules in the development of mouse central nervous system.
Neurochem Res. 38 (2): 290-9. -
Lv, D. et al. (2014) The similar expression pattern of MHC class I molecules in human and mouse cerebellar cortex.
Neurochem Res. 39 (1): 180-6. -
Sobue, A. et al. (2018) Astroglial major histocompatibility complex class I following immune activation leads to behavioral and neuropathological changes.
Glia. 66 (5): 1034-52.
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