MHC Class I H-2b/D/P/Q/w16 antibody | ER-HR52
Clone ER-HR52 specifically recognizes mouse strains with the haplotypes H-2b, w16 and H-2d, p, q. Mouse strains with the haplotypes H-2f, r, s, w17, w23, w27 show weak reactivity with this antibody.
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Adherent FI (CBAXBL) bone marrow stromal cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cells from immunised rats were fused with cells of the mouse P3-X63-Ag8.563 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of MHC Class I H-2b/D/P/Q/w16 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/50 | 1/200 | |
Immunofluorescence | |||
Immunohistology - Frozen |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
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Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
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Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
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Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
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Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
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Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
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Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
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Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
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Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
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Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
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Product Specific References
References for MHC Class I H-2b/D/P/Q/w16 antibody
-
Medana, I. et al. (2001) Transection of major histocompatibility complex class I-induced neurites by cytotoxic T lymphocytes.
Am J Pathol. 159 (3): 809-15. -
Lv, D. et al. (2015) Neuronal MHC Class I Expression Is Regulated by Activity Driven Calcium Signaling.
PLoS One. 10 (8): e0135223. -
Himoudi, N. et al. (2009) Migratory and antigen presentation functions of IFN-producing killer dendritic cells.
Cancer Res. 69 (16): 6598-606. -
Liu, J. et al. (2015) Spatial-Temporal Expression of Non-classical MHC Class I Molecules in the C57 Mouse Brain.
Neurochem Res. 40 (7): 1487-96. -
Liu, J. et al. (2013) The expression pattern of classical MHC class I molecules in the development of mouse central nervous system.
Neurochem Res. 38 (2): 290-9. -
Lv, D. et al. (2014) The similar expression pattern of MHC class I molecules in human and mouse cerebellar cortex.
Neurochem Res. 39 (1): 180-6. -
Sobue, A. et al. (2018) Astroglial major histocompatibility complex class I following immune activation leads to behavioral and neuropathological changes.
Glia. Jan 30 [Epub ahead of print].
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