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MHC Class I H-2b/D/P/Q/w16 antibody | ER-HR52

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Anti Mouse MHC Class I H-2b/D/P/Q/w16 Antibody, clone ER-HR52 gallery image 1
Published customer image:
Rat anti Mouse MHC Class I H-2b/D/P/Q/w16 antibody, clone ER-HR52 (MCA2398) used for the identification of MHC class I expressing cells in mouse brain by immunofluorescence.
Image caption:
Dynamic expression of H-2Kb/Db in mouse hippocampus.
(A) At P15, MHCI protein signals (green) were overlapped with those of NeuN (red, a marker for neurons) but not with GFAP (red, a marker for astrocytes). Scale bar: 50 μm. (B) The mRNA of H-2Kb and H-2Db was dynamically expressed in mouse hippocampus. Data was shown as ratio compared with P4 and was calculated from three independent experiments. (C) Western blot analysis depicting the expression of MHC-I protein during the development stages of hippocampus. (D) Expression of MHC-I protein was quantified as the ratio of band density to that of Tuj1. Data was presented as ratio compared with P4 and was calculated from three independent experiments.

From: Lv D, Shen Y, Peng Y, Liu J, Miao F, Zhang J (2015) Neuronal MHC Class I Expression Is Regulated by Activity Driven Calcium Signaling.
PLoS ONE 10(8): e0135223.

Rat anti Mouse MHC Class I H-2b/D/P/Q/w16

Product Type
Monoclonal Antibody
Clone
ER-HR52
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA2398
Datasheet
C F IF
SDS
0.25 mg loader

Rat anti Mouse MHC Class I H-2b/D/P/Q/w16 antibody, clone ER-HR52 recognizes a polymorphic epitope present on murine MHC class I molecules, which are expressed at varying levels on the majority of nucleated cells.

Clone ER-HR52 specifically recognizes mouse strains with the haplotypes H-2b, w16 and H-2d, p, q. Mouse strains with the haplotypes H-2f, r, s, w17, w23, w27 show weak reactivity with this antibody.

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region.

Product Details

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Adherent FI (CBAXBL) bone marrow stromal cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cells from immunised rats were fused with cells of the mouse P3-X63-Ag8.563 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of MHC Class I H-2b/D/P/Q/w16 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/200
Immunofluorescence
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available

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Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Rat IgG2a Negative Control MCA1212 E F 1 ml loader

Product Specific References

References for MHC Class I H-2b/D/P/Q/w16 antibody

  1. Medana, I. et al. (2001) Transection of major histocompatibility complex class I-induced neurites by cytotoxic T lymphocytes.
    Am J Pathol. 159 (3): 809-15.
  2. Lv, D. et al. (2015) Neuronal MHC Class I Expression Is Regulated by Activity Driven Calcium Signaling.
    PLoS One. 10 (8): e0135223.
  3. Himoudi, N. et al. (2009) Migratory and antigen presentation functions of IFN-producing killer dendritic cells.
    Cancer Res. 69 (16): 6598-606.
  4. Liu, J. et al. (2015) Spatial-Temporal Expression of Non-classical MHC Class I Molecules in the C57 Mouse Brain.
    Neurochem Res. 40 (7): 1487-96.
  5. Liu, J. et al. (2013) The expression pattern of classical MHC class I molecules in the development of mouse central nervous system.
    Neurochem Res. 38 (2): 290-9.
  6. Lv, D. et al. (2014) The similar expression pattern of MHC class I molecules in human and mouse cerebellar cortex.
    Neurochem Res. 39 (1): 180-6.
  7. Sobue, A. et al. (2018) Astroglial major histocompatibility complex class I following immune activation leads to behavioral and neuropathological changes.
    Glia. Jan 30 [Epub ahead of print].

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