Rat anti Mouse MHC Class I H-2b/D/P/Q/w16 antibody, clone ER-HR52 recognizes a polymorphic epitope present on murine MHC class I molecules, which are expressed at varying levels on the majority of nucleated cells.
Clone ER-HR52 specifically recognizes mouse strains with the haplotypes H-2b, w16 and H-2d, p, q. Mouse strains with the haplotypes H-2f, r, s, w17, w23, w27 show weak reactivity with this antibody.
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Carrier Free
- Adherent FI (CBAXBL) bone marrow stromal cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cells from immunised rats were fused with cells of the mouse P3-X63-Ag8.563 myeloma cell line.
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of MHC Class I H-2b/D/P/Q/w16 antibody
|Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
Product Specific References
References for MHC Class I H-2b/D/P/Q/w16 antibody
Medana, I. et al. (2001) Transection of major histocompatibility complex class I-induced neurites by cytotoxic T lymphocytes.
Am J Pathol. 159 (3): 809-15.
Lv, D. et al. (2015) Neuronal MHC Class I Expression Is Regulated by Activity Driven Calcium Signaling.
PLoS One. 10 (8): e0135223.
Himoudi, N. et al. (2009) Migratory and antigen presentation functions of IFN-producing killer dendritic cells.
Cancer Res. 69 (16): 6598-606.
Liu, J. et al. (2015) Spatial-Temporal Expression of Non-classical MHC Class I Molecules in the C57 Mouse Brain.
Neurochem Res. 40 (7): 1487-96.
Liu, J. et al. (2013) The expression pattern of classical MHC class I molecules in the development of mouse central nervous system.
Neurochem Res. 38 (2): 290-9.
Lv, D. et al. (2014) The similar expression pattern of MHC class I molecules in human and mouse cerebellar cortex.
Neurochem Res. 39 (1): 180-6.
Sobue, A. et al. (2018) Astroglial major histocompatibility complex class I following immune activation leads to behavioral and neuropathological changes.
Glia. 66 (5): 1034-52.
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