M31 antibody | MAC353

Rat anti M31/HP1

Product Type
Monoclonal Antibody
Clone
MAC353
Isotype
IgG2b
Specificity
M31

Product Code Applications Pack Size List Price Your Price Qty
MCA1946
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C Ch E IF IP WB 2 ml loader
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loader
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Rat anti Mouse M31 antibody, clone MAC353 recognises the M31 molecule in mouse and the homologous Chromobox protein homolog 1 or HP1Hsbeta molecule in man. Rat anti Mouse M31 antibody, clone MAC353 has been reported for use in western blotting and recognises a ~26 kDa protein in murine and human nuclear extracts (Wreggett et al. 1994).

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
71 amino acid fusion protein corresponding to the C-terminal region of M31
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Chromatin Immunoprecipitation
ELISA
Immunofluorescence
Immunohistology - Frozen Neat 1/50
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for M31 antibody

  1. Wreggett, K.A. et al. (1994) A mammalian homologue of Drosophila heterochromatin protein 1 (HP1) is a component of constitutive heterochromatin.
    Cytogenet Cell Genet. 66 (2): 99-103.
  2. Sabbattini, P. et al. (2007) A novel role for the Aurora B kinase in epigenetic marking of silent chromatin in differentiated postmitotic cells.
    EMBO J. 26 (22): 4657-69.
  3. Epsztejn-Litman, S. et al. (2008) De novo DNA methylation promoted by G9a prevents reprogramming of embryonically silenced genes.
    Nat Struct Mol Biol. 15: 1176-83.
  4. Matsui, T. et al. (2010) Proviral silencing in embryonic stem cells requires the histone methyltransferase ESET.
    Nature. 464: 927-31.
  5. Frohnert, C. et al. (2011) SPAG4L/SPAG4L-2 are testis-specific SUN domain proteins restricted to the apical nuclear envelope of round spermatids facing the acrosome.
    Mol Hum Reprod. 17: 207-18.
  6. Baxter, J. et al. (2004) Histone hypomethylation is an indicator of epigenetic plasticity in quiescent lymphocytes.
    EMBO J. 23 (22): 4462-72.
  7. McDowell, T.L. et al. (1999) Localization of a putative transcriptional regulator (ATRX) at pericentromeric heterochromatin and the short arms of acrocentric chromosomes.
    Proc Natl Acad Sci U S A. 96: 13983-8.
  8. Sabbattini, P. et al. (2007) A novel role for the Aurora B kinase in epigenetic marking of silent chromatin in differentiated postmitotic cells.
    EMBO J. 26 (22): 4657-69.
  9. View The Latest Product References
  10. Groves, I.J. et al. (2009) Lytic infection of permissive cells with human cytomegalovirus is regulated by an intrinsic 'pre-immediate-early' repression of viral gene expression mediated by histone post-translational modification.
    J Gen Virol. 90: 2364-74.
  11. Groves, I.J. and Sinclair, J.H. (2007) Knockdown of hDaxx in normally non-permissive undifferentiated cells does not permit human cytomegalovirus immediate-early gene expression.
    J Gen Virol. 88: 2935-40.
  12. Abe, K. et al. (2011) Loss of heterochromatin protein 1 gamma reduces the number of primordial germ cells via impaired cell cycle progression in mice.
    Biol Reprod. 85 (5): 1013-24.

Immunofluorescence

Western Blotting

Synonyms
Heterochromatin Protein 1 Homolog Beta
RRID
AB_2259687
UniProt
P83917
Entrez Gene
Cbx1
GO Terms
GO:0001939 female pronucleus
GO:0001940 male pronucleus
GO:0003682 chromatin binding
GO:0006333 chromatin assembly or disassembly
GO:0005721 centromeric heterochromatin
GO:0042802 identical protein binding
GO:0010369 chromocenter
GO:0016564 transcription repressor activity
GO:0045892 negative regulation of transcription, DNA-dependent
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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