M31 antibody | MAC353
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Rat anti Mouse M31 antibody, clone MAC353 recognises the M31 molecule in mouse and the homologous Chromobox protein homolog 1 or HP1Hsbeta molecule in man. Rat anti Mouse M31 antibody, clone MAC353 has been reported for use in western blotting and recognises a ~26 kDa protein in murine and human nuclear extracts (Wreggett et al. 1994).
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Tissue Culture Supernatant - liquid
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- 71 amino acid fusion protein corresponding to the C-terminal region of M31
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P83917
- Entrez Gene
- Cbx1
- GO Terms
- GO:0001939 female pronucleus
- GO:0001940 male pronucleus
- GO:0003682 chromatin binding
- GO:0006333 chromatin assembly or disassembly
- GO:0005721 centromeric heterochromatin
- GO:0042802 identical protein binding
- GO:0010369 chromocenter
- GO:0016564 transcription repressor activity
- GO:0045892 negative regulation of transcription, DNA-dependent
- Regulatory
- For research purposes only
Applications of M31 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Chromatin Immunoprecipitation | |||
| ELISA | |||
| Immunofluorescence | |||
| Immunohistology - Frozen | Neat | 1/50 | |
| Immunoprecipitation | |||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2022 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
|
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| Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
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| Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
|
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| Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
|
|
| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
|
|
| Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) | STAR71D650 | F IF | 0.1 mg |
|
|
| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
|
|
| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
|
|
| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
|
Product Specific References
References for M31 antibody
-
Wreggett, K.A. et al. (1994) A mammalian homologue of Drosophila heterochromatin protein 1 (HP1) is a component of constitutive heterochromatin.
Cytogenet Cell Genet. 66 (2): 99-103. -
Sabbattini, P. et al. (2007) A novel role for the Aurora B kinase in epigenetic marking of silent chromatin in differentiated postmitotic cells.
EMBO J. 26 (22): 4657-69. -
Epsztejn-Litman, S. et al. (2008) De novo DNA methylation promoted by G9a prevents reprogramming of embryonically silenced genes.
Nat Struct Mol Biol. 15: 1176-83. -
Matsui, T. et al. (2010) Proviral silencing in embryonic stem cells requires the histone methyltransferase ESET.
Nature. 464: 927-31. -
Frohnert, C. et al. (2011) SPAG4L/SPAG4L-2 are testis-specific SUN domain proteins restricted to the apical nuclear envelope of round spermatids facing the acrosome.
Mol Hum Reprod. 17: 207-18. -
Baxter, J. et al. (2004) Histone hypomethylation is an indicator of epigenetic plasticity in quiescent lymphocytes.
EMBO J. 23 (22): 4462-72. -
McDowell, T.L. et al. (1999) Localization of a putative transcriptional regulator (ATRX) at pericentromeric heterochromatin and the short arms of acrocentric chromosomes.
Proc Natl Acad Sci U S A. 96: 13983-8. -
Sabbattini, P. et al. (2007) A novel role for the Aurora B kinase in epigenetic marking of silent chromatin in differentiated postmitotic cells.
EMBO J. 26 (22): 4657-69. -
Groves, I.J. et al. (2009) Lytic infection of permissive cells with human cytomegalovirus is regulated by an intrinsic 'pre-immediate-early' repression of viral gene expression mediated by histone post-translational modification.
J Gen Virol. 90: 2364-74. -
Groves, I.J. and Sinclair, J.H. (2007) Knockdown of hDaxx in normally non-permissive undifferentiated cells does not permit human cytomegalovirus immediate-early gene expression.
J Gen Virol. 88: 2935-40. -
Abe, K. et al. (2011) Loss of heterochromatin protein 1 gamma reduces the number of primordial germ cells via impaired cell cycle progression in mice.
Biol Reprod. 85 (5): 1013-24.
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