M31 antibody | MAC353
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Rat anti M31/HP1
- Product Type
- Monoclonal Antibody
- Clone
- MAC353
- Isotype
- IgG2b
- Specificity
- M31
Antibody Quality and Performance Guarantee
| Rat anti Mouse M31 antibody, clone MAC353 recognises the M31 molecule in mouse and the homologous Chromobox protein homolog 1 or HP1Hsbeta molecule in man. Rat anti Mouse M31 antibody, clone MAC353 has been reported for use in western blotting and recognises a ~26 kDa protein in murine and human nuclear extracts (Wreggett et al. 1994). |
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Tissue culture supernatant - liquid
- Preservative Stabilisers
- 0.1% sodium azide (NaN3)
- Immunogen
- 71 amino acid fusion protein corresponding to the C-terminal region of M31
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Chromatin Immunoprecipitation |  |
||
| ELISA | |
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| Immunofluorescence | |
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| Immunohistology - Frozen | |
Neat | 1/50 |
| Immunoprecipitation | |
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| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for M31 antibody
-
Wreggett, K.A. et al. (1994) A mammalian homologue of Drosophila heterochromatin protein 1 (HP1) is a component of constitutive heterochromatin.
Cytogenet Cell Genet. 66 (2): 99-103. -
Sabbattini, P. et al. (2007) A novel role for the Aurora B kinase in epigenetic marking of silent chromatin in differentiated postmitotic cells.
EMBO J. 26 (22): 4657-69. -
Epsztejn-Litman, S. et al. (2008) De novo DNA methylation promoted by G9a prevents reprogramming of embryonically silenced genes.
Nat Struct Mol Biol. 15: 1176-83. -
Matsui, T. et al. (2010) Proviral silencing in embryonic stem cells requires the histone methyltransferase ESET.
Nature. 464: 927-31. -
Frohnert, C. et al. (2011) SPAG4L/SPAG4L-2 are testis-specific SUN domain proteins restricted to the apical nuclear envelope of round spermatids facing the acrosome.
Mol Hum Reprod. 17: 207-18. -
Baxter, J. et al. (2004) Histone hypomethylation is an indicator of epigenetic plasticity in quiescent lymphocytes.
EMBO J. 23 (22): 4462-72. -
McDowell, T.L. et al. (1999) Localization of a putative transcriptional regulator (ATRX) at pericentromeric heterochromatin and the short arms of acrocentric chromosomes.
Proc Natl Acad Sci U S A. 96: 13983-8. -
Sabbattini, P. et al. (2007) A novel role for the Aurora B kinase in epigenetic marking of silent chromatin in differentiated postmitotic cells.
EMBO J. 26 (22): 4657-69.
View The Latest Product References
-
Groves, I.J. et al. (2009) Lytic infection of permissive cells with human cytomegalovirus is regulated by an intrinsic 'pre-immediate-early' repression of viral gene expression mediated by histone post-translational modification.
J Gen Virol. 90: 2364-74. -
Groves, I.J. and Sinclair, J.H. (2007) Knockdown of hDaxx in normally non-permissive undifferentiated cells does not permit human cytomegalovirus immediate-early gene expression.
J Gen Virol. 88: 2935-40. -
Abe, K. et al. (2011) Loss of heterochromatin protein 1 gamma reduces the number of primordial germ cells via impaired cell cycle progression in mice.
Biol Reprod. 85 (5): 1013-24. -
Ballmer, D. et al. (2022) HP1 proteins regulate nucleolar structure and function by secluding pericentromeric constitutive heterochromatin.
Nucleic Acids Res. Dec 19 [Epub ahead of print].
- Synonyms
- Heterochromatin Protein 1 Homolog Beta
- RRID
- AB_2259687
- UniProt
- P83917
- Entrez Gene
- Cbx1
- GO Terms
- GO:0001939 female pronucleus
- GO:0001940 male pronucleus
- GO:0003682 chromatin binding
- GO:0006333 chromatin assembly or disassembly
- GO:0005721 centromeric heterochromatin
- GO:0042802 identical protein binding
- GO:0010369 chromocenter
- GO:0016564 transcription repressor activity
- GO:0045892 negative regulation of transcription, DNA-dependent
- View More GO Terms
MCA1946
155611
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