Lymphotoxin Beta Receptor antibody | 5G11b

Rat anti Mouse Lymphotoxin Beta Receptor

Product Type
Monoclonal Antibody
Clone
5G11b
Isotype
IgG2a
Specificity
Lymphotoxin Beta Receptor

Product Code Applications Pack Size List Price Your Price Qty
MCA2244GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader
MCA2244
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.25 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rat anti Mouse Lymphotoxin beta Receptor antibody, clone 5G11b recognizes murine Tumor necrosis factor receptor superfamily member 3, also known as lymphotoxin-beta receptor, LT beta R, or Tumor necrosis factor receptor superfamily member 3. Lymphotoxin beta receptor is 385 amino acid (415 aa including 30 aa signal peptide) ~50 kDa type 1, single pass transmembrane glycoprotein and member of the tumor necrosis factor family. LT beta R is primarily expressed on stromal cells and fibroblasts and at lower levels on myeloid cell lines. Rat anti Mouse Lymphotoxin beta Receptor antibody, clone 5G11b recognizes surface expressed LT beta R.

Rat anti Mouse Lymphotoxin beta Receptor antibody, clone 5G11b is reported to be agonistic with respect to NF Kappa B activation, MIP-2 induction and IL-6 release (Hehlgans et al. 2003).

Rat anti Mouse Lymphotoxin beta Receptor antibody, clone 5G11b is routinely tested on WEH164S cells.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
Recombinant extracellular domain of LT beta R.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Wistar rats were fused with cells of the SP2/0 - Ag14 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
List Price Your Price
loader
Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Rat IgG:FITC
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
List Price Your Price
loader
Description Goat anti Rat IgG:Biotin (Mouse Adsorbed)
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader
List Price Your Price
loader
Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
List Price Your Price
loader
Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) STAR71D650 F IF 0.1 mg loader
List Price Your Price
loader
Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Rat IgG:HRP
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Rat IgG:Dylight®800
Goat anti Rat IgG:DyLight®550 (Mouse Adsorbed) STAR71D550 F IF WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Rat IgG:DyLight®550 (Mouse Adsorbed)
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
List Price Your Price
loader
Description Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control MCA1212 E F 1 ml loader
List Price Your Price
loader
Description Rat IgG2a Negative Control

Source Reference

  1. Hehlgans T et al. (2003) Activation of the lymphotoxin-beta receptor induces NFkappaB-dependent interleukin-6 and MIP-2 secretion in mouse fibrosarcoma cells.
    Eur Cytokine Netw. 14 (2): 103-7.

References for Lymphotoxin Beta Receptor antibody

  1. Palumbo, M.O. et al. (2006) Isolation and characterization of proinsulin-producing medullary thymic epithelial cell clones.
    Diabetes. 55: 2595-601.
  2. Moore, F. et al. (2007) An alternative pathway of NF-kappaB activation results in maturation and T cell priming activity of dendritic cells overexpressing a mutated IkappaBalpha.
    J Immunol. 178: 1301-11.
  3. Magari, M. et al. (2011) IL-21-Dependent B Cell Death Driven by Prostaglandin E2, a Product Secreted from Follicular Dendritic Cells.
    J Immunol. 187: 4210-8.
  4. Into, T. et al. (2018) MyD88 signaling causes autoimmune sialadenitis through formation of high endothelial venules and upregulation of LTββ receptor-mediated signaling.
    Sci Rep. 8 (1): 14272.

Further Reading

  1. Muller, P. et al. (2001) Functional characterization of the mouse lymphotoxin-beta receptor promoter.
    Eur Cytokine Netw. 12 (2): 325-30.

RRID
AB_566581
UniProt
P50284
Entrez Gene
Ltbr
GO Terms
GO:0005515 protein binding
GO:0006915 apoptosis
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0048535 lymph node development