Lymphotoxin Beta Receptor antibody | 5G11b
Low endotoxin Rat anti Mouse lymphotoxin beta receptor antibody, clone 5G11b (MCA2244EL) used to stimulate mouse embryonic fibroblast cultures prior to protein expression assessment by western blotting.
Image caption:
(c) Myd88+/+ and Myd88−/− MEFs were stimulated with 2.5 μg/mL LTβR agonistic antibody for indicated periods, followed by lysis, SDS-PAGE and immunoblotting to assess levels of NIK, NF-κB2 (p100/p52), TRAF3, and Tubulin. Representative results of at least three independent experiments are shown. All the blots were obtained under the same experimental conditions, and the cropped images of the blots are shown.
From: Into T, Niida S, Shibata KI.
MyD88 signaling causes autoimmune sialadenitis through formation of high endothelial venules and upregulation of LTβ receptor-mediated signaling.
Sci Rep. 2018 Sep 24;8(1):14272.
doi: 10.1038/s41598-018-32690-x.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse Lymphotoxin Beta Receptor
- Product Type
- Monoclonal Antibody
- Clone
- 5G11b
- Isotype
- IgG2a
- Specificity
- Lymphotoxin Beta Receptor
Antibody Quality and Performance Guarantee
| Rat anti Mouse Lymphotoxin beta Receptor antibody, clone 5G11b recognizes murine Tumor necrosis factor receptor superfamily member 3, also known as lymphotoxin-beta receptor, LT beta R, or Tumor necrosis factor receptor superfamily member 3. Lymphotoxin beta receptor is 385 amino acid (415 aa including 30 aa signal peptide) ~50 kDa type 1, single pass transmembrane glycoprotein and member of the tumor necrosis factor family. LT beta R is primarily expressed on stromal cells and fibroblasts and at lower levels on myeloid cell lines. Rat anti Mouse Lymphotoxin beta Receptor antibody, clone 5G11b recognizes surface expressed LT beta R. Rat anti Mouse Lymphotoxin beta Receptor antibody, clone 5G11b is reported to be agonistic with respect to NF Kappa B activation, MIP-2 induction and IL-6 release (Hehlgans et al. 2003). Rat anti Mouse Lymphotoxin beta Receptor antibody, clone 5G11b is routinely tested on WEH164S cells. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Recombinant extracellular domain of LT beta R.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized Wistar rats were fused with cells of the SP2/0 - Ag14 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
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| List Price | Your Price | ||||||
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| Description | Rat IgG2a Negative Control | ||||||
Source Reference
-
Hehlgans T et al. (2003) Activation of the lymphotoxin-beta receptor induces NFkappaB-dependent interleukin-6 and MIP-2 secretion in mouse fibrosarcoma cells.
Eur Cytokine Netw. 14 (2): 103-7.
References for Lymphotoxin Beta Receptor antibody
-
Palumbo, M.O. et al. (2006) Isolation and characterization of proinsulin-producing medullary thymic epithelial cell clones.
Diabetes. 55: 2595-601. -
Moore, F. et al. (2007) An alternative pathway of NF-kappaB activation results in maturation and T cell priming activity of dendritic cells overexpressing a mutated IkappaBalpha.
J Immunol. 178: 1301-11. -
Magari, M. et al. (2011) IL-21-Dependent B Cell Death Driven by Prostaglandin E2, a Product Secreted from Follicular Dendritic Cells.
J Immunol. 187: 4210-8. -
Into, T. et al. (2018) MyD88 signaling causes autoimmune sialadenitis through formation of high endothelial venules and upregulation of LTββ receptor-mediated signaling.
Sci Rep. 8 (1): 14272.
Further Reading
-
Muller, P. et al. (2001) Functional characterization of the mouse lymphotoxin-beta receptor promoter.
Eur Cytokine Netw. 12 (2): 325-30.
- RRID
- AB_566581
- UniProt
- P50284
- Entrez Gene
- Ltbr
- GO Terms
- GO:0005515 protein binding
- GO:0006915 apoptosis
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0048535 lymph node development
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