Lymphotoxin Beta Receptor antibody | 5G11b
Low endotoxin Rat anti Mouse lymphotoxin beta receptor antibody, clone 5G11b (MCA2244EL) used to stimulate mouse embryonic fibroblast cultures prior to protein expression assessment by western blotting.
Image caption:
(c) Myd88+/+ and Myd88−/− MEFs were stimulated with 2.5 μg/mL LTβR agonistic antibody for indicated periods, followed by lysis, SDS-PAGE and immunoblotting to assess levels of NIK, NF-κB2 (p100/p52), TRAF3, and Tubulin. Representative results of at least three independent experiments are shown. All the blots were obtained under the same experimental conditions, and the cropped images of the blots are shown.
From: Into T, Niida S, Shibata KI.
MyD88 signaling causes autoimmune sialadenitis through formation of high endothelial venules and upregulation of LTβ receptor-mediated signaling.
Sci Rep. 2018 Sep 24;8(1):14272.
doi: 10.1038/s41598-018-32690-x.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse Lymphotoxin Beta Receptor:Alexa Fluor® 647
Rat anti Mouse Lymphotoxin Beta Receptor:Low Endotoxin
Rat anti Mouse Lymphotoxin Beta Receptor
Rat anti Mouse Lymphotoxin Beta Receptor:RPE
- Product Type
- Monoclonal Antibody
- Clone
- 5G11b
- Isotype
- IgG2a
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA2244A647 | F | 100 Tests/1ml |
|
|
| MCA2244EL | F FN | 0.5 mg |
|
|
| MCA2244GA | F | 0.1 mg |
|
|
| MCA2244 | F | 0.25 mg |
|
|
| MCA2244PE | F | 100 Tests |
|
Rat anti Mouse Lymphotoxin beta Receptor antibody, clone 5G11b recognizes murine lymphotoxin-beta receptor (LT beta R), a member of the tumor necrosis factor family. LT beta R is primarily expressed on stromal cells and fibroblasts and at lower levels on myeloid cell lines. Rat anti Mouse Lymphotoxin beta Receptor antibody, clone 5G11b recognizes surface expressed LT beta R.
Rat anti Mouse Lymphotoxin beta Receptor antibody, clone 5G11b is reported to be agonistic with respect to NF Kappa B activation, MIP-2 induction and IL-6 release (Hehlgans et al. 2003).
Rat anti Mouse Lymphotoxin beta Receptor antibody, clone 5G11b is routinely tested on WEH1 cells.
Rat anti Mouse Lymphotoxin beta Receptor antibody, clone 5G11b is reported to be agonistic with respect to NF Kappa B activation, MIP-2 induction and IL-6 release (Hehlgans et al. 2003).
Rat anti Mouse Lymphotoxin beta Receptor antibody, clone 5G11b is routinely tested on WEH1 cells.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Alexa Fluor 647 - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Recombinant extracellular domain of LT beta R.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Wistar rats were fused with cells of the SP2/0 - Ag14 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use - Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 12 months from date of reconstitution.
More Information
- UniProt
- P50284 Related reagents
- Entrez Gene
- Ltbr Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0006915 apoptosis
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0048535 lymph node development
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of Lymphotoxin Beta Receptor antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Flow Cytometry | 1/50 | 1/100 | |
| Functional Assays | |||
| Flow Cytometry | 1/50 | 1/100 | |
| Flow Cytometry | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B). - Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rat IgG2a Negative Control:Alexa Fluor® 647 | MCA1212A647 | F | 100 Tests/1ml |
|
|
| Rat IgG2a Negative Control:Low Endotoxin | MCA1212EL | F | 0.5 mg |
|
|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
|
|
| Rat IgG2a Negative Control:RPE | MCA1212PE | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
|
|
| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
|
|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
|
|
| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
|
Product Specific References
References for Lymphotoxin Beta Receptor antibody
-
Hehlgans T et al. (2003) Activation of the lymphotoxin-beta receptor induces NFkappaB-dependent interleukin-6 and MIP-2 secretion in mouse fibrosarcoma cells.
Eur Cytokine Netw. 14 (2): 103-7. -
Palumbo, M.O. et al. (2006) Isolation and characterization of proinsulin-producing medullary thymic epithelial cell clones.
Diabetes. 55: 2595-601. -
Moore, F. et al. (2007) An alternative pathway of NF-kappaB activation results in maturation and T cell priming activity of dendritic cells overexpressing a mutated IkappaBalpha.
J Immunol. 178: 1301-11. -
Magari, M. et al. (2011) IL-21-Dependent B Cell Death Driven by Prostaglandin E2, a Product Secreted from Follicular Dendritic Cells.
J Immunol. 187: 4210-8. -
Into, T. et al. (2018) MyD88 signaling causes autoimmune sialadenitis through formation of high endothelial venules and upregulation of LTββ receptor-mediated signaling.
Sci Rep. 8 (1): 14272.
Further Reading
-
Muller, P. et al. (2001) Functional characterization of the mouse lymphotoxin-beta receptor promoter.
Eur Cytokine Netw. 12 (2): 325-30.
