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Lymphotoxin Beta Receptor antibody | 5G11b

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Anti Mouse Lymphotoxin Beta Receptor Antibody, clone 5G11b thumbnail image 1 Anti Mouse Lymphotoxin Beta Receptor Antibody, clone 5G11b thumbnail image 2
Anti Mouse Lymphotoxin Beta Receptor Antibody, clone 5G11b gallery image 1
Figure A. Alexa Fluor 488 conjugated Mouse anti Mouse CD90 (MCA02) and Alexa Fluor 647 conjugated Rat IgG2a isotype control (MCA1212A647). Figure B . Alexa Fluor 488 conjugated Mouse anti Mouse CD90 (MCA1940F) and Alexa Fluor 647 conjugated Rat anti Mouse LTbetaR (MCA2244A647). All experiments performed on WEHI-164S cells gated on live single cells, in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer
Anti Mouse Lymphotoxin Beta Receptor Antibody, clone 5G11b gallery image 2
Published customer image:
Low endotoxin Rat anti Mouse lymphotoxin beta receptor antibody, clone 5G11b (MCA2244EL) used to stimulate mouse embryonic fibroblast cultures prior to protein expression assessment by western blotting.
Image caption:
(c) Myd88+/+ and Myd88−/− MEFs were stimulated with 2.5 μg/mL LTβR agonistic antibody for indicated periods, followed by lysis, SDS-PAGE and immunoblotting to assess levels of NIK, NF-κB2 (p100/p52), TRAF3, and Tubulin. Representative results of at least three independent experiments are shown. All the blots were obtained under the same experimental conditions, and the cropped images of the blots are shown.

From: Into T, Niida S, Shibata KI.
MyD88 signaling causes autoimmune sialadenitis through formation of high endothelial venules and upregulation of LTβ receptor-mediated signaling.
Sci Rep. 2018 Sep 24;8(1):14272.
doi: 10.1038/s41598-018-32690-x.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Rat anti Mouse Lymphotoxin Beta Receptor:Alexa Fluor® 647

Rat anti Mouse Lymphotoxin Beta Receptor:Low Endotoxin

Rat anti Mouse Lymphotoxin Beta Receptor

Rat anti Mouse Lymphotoxin Beta Receptor:RPE

Product Type
Monoclonal Antibody
Clone
5G11b
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA2244A647
Datasheet
F
SDS
100 Tests/1ml loader

MCA2244EL
Datasheet
F FN
SDS
0.5 mg loader

MCA2244GA
Datasheet
F
SDS
0.1 mg loader

MCA2244
Datasheet
F
SDS
0.25 mg loader

MCA2244PE
Datasheet
F
SDS
100 Tests loader

Rat anti Mouse Lymphotoxin beta Receptor antibody, clone 5G11b recognizes murine Tumor necrosis factor receptor superfamily member 3, also known as lymphotoxin-beta receptor, LT beta R, or Tumor necrosis factor receptor superfamily member 3. Lymphotoxin beta receptor is 385 amino acid (415 aa including 30 aa signal peptide) ~50 kDa type 1, single pass transmembrane glycoprotein and member of the tumor necrosis factor family. LT beta R is primarily expressed on stromal cells and fibroblasts and at lower levels on myeloid cell lines. Rat anti Mouse Lymphotoxin beta Receptor antibody, clone 5G11b recognizes surface expressed LT beta R.

Rat anti Mouse Lymphotoxin beta Receptor antibody, clone 5G11b is reported to be agonistic with respect to NF Kappa B activation, MIP-2 induction and IL-6 release (Hehlgans et al. 2003).

Rat anti Mouse Lymphotoxin beta Receptor antibody, clone 5G11b is routinely tested on WEH164S cells.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Alexa Fluor 647 - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
None present
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Carrier Free
Yes
Immunogen
Recombinant extracellular domain of LT beta R.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Wistar rats were fused with cells of the SP2/0 - Ag14 mouse myeloma cell line.

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
Store at -20oC only.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P50284
Entrez Gene
Ltbr
GO Terms
GO:0005515 protein binding
GO:0006915 apoptosis
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0048535 lymph node development
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of Lymphotoxin Beta Receptor antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry 1/50 1/100
Functional Assays
Flow Cytometry 1/50 1/100
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Rat IgG2a Negative Control:Alexa Fluor® 647 MCA1212A647 F 100 Tests/1ml loader
Rat IgG2a Negative Control:Low Endotoxin MCA1212EL F 0.5 mg loader
Rat IgG2a Negative Control MCA1212 E F 1 ml loader
Rat IgG2a Negative Control:RPE MCA1212PE F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader

Product Specific References

Source Reference

  1. Hehlgans T et al. (2003) Activation of the lymphotoxin-beta receptor induces NFkappaB-dependent interleukin-6 and MIP-2 secretion in mouse fibrosarcoma cells.
    Eur Cytokine Netw. 14 (2): 103-7.

References for Lymphotoxin Beta Receptor antibody

  1. Palumbo, M.O. et al. (2006) Isolation and characterization of proinsulin-producing medullary thymic epithelial cell clones.
    Diabetes. 55: 2595-601.
  2. Moore, F. et al. (2007) An alternative pathway of NF-kappaB activation results in maturation and T cell priming activity of dendritic cells overexpressing a mutated IkappaBalpha.
    J Immunol. 178: 1301-11.
  3. Magari, M. et al. (2011) IL-21-Dependent B Cell Death Driven by Prostaglandin E2, a Product Secreted from Follicular Dendritic Cells.
    J Immunol. 187: 4210-8.
  4. Into, T. et al. (2018) MyD88 signaling causes autoimmune sialadenitis through formation of high endothelial venules and upregulation of LTββ receptor-mediated signaling.
    Sci Rep. 8 (1): 14272.

Further Reading

  1. Muller, P. et al. (2001) Functional characterization of the mouse lymphotoxin-beta receptor promoter.
    Eur Cytokine Netw. 12 (2): 325-30.

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